A5053969945- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Diet and metabolism studies
- Bacteriophages and microbial interactions
- Gastrointestinal motility and disorders
- Diabetes and associated disorders
- Genomics and Phylogenetic Studies
- Nutritional Studies and Diet
- Respiratory viral infections research
- Plant Virus Research Studies
- Immune Cell Function and Interaction
- Pharmacological Effects of Natural Compounds
- Tryptophan and brain disorders
- Phytochemicals and Antioxidant Activities
- Nuclear Structure and Function
- Liver Disease Diagnosis and Treatment
- Sodium Intake and Health
- GABA and Rice Research
- MicroRNA in disease regulation
- Botulinum Toxin and Related Neurological Disorders
- Parasitic Infections and Diagnostics
- Digestive system and related health
- Nutrition, Genetics, and Disease
- Plant Toxicity and Pharmacological Properties
- Natural Antidiabetic Agents Studies
Amsterdam University Medical Centers
2020-2025
University of Amsterdam
2020-2025
Amsterdam University of the Arts
2025
Amsterdam Neuroscience
2024
Amsterdam UMC Location University of Amsterdam
2019-2021
Abstract Dysbiosis of the intestinal microbiota has been implicated in insulin resistance, although evidence regarding causality humans is scarce. We performed a phase I/II dose-finding and safety study on effect oral intake anaerobic butyrogenic strain Anaerobutyricum soehngenii glucose metabolism 24 subjects with metabolic syndrome. found that treatment A. was safe observed significant correlation between measured fecal abundance administered improvement peripheral sensitivity after 4...
Abstract There is significant interest in altering the course of cardiometabolic disease development via gut microbiomes. Nevertheless, highly abundant phage members complex ecosystem -which impact bacteria- remain understudied. Here, we show virome changes associated with metabolic syndrome (MetS), a prevalent clinical condition preceding disease, 196 participants by combined sequencing bulk whole genome and virus like particle communities. MetS viromes exhibit decreased richness diversity....
Bacteriophages (phages) are bacterial viruses that have been shown to shape microbial communities. Previous studies faecal virome transplantation can decrease weight gain and normalize blood glucose tolerance in diet-induced obese mice. Therefore, we performed a double-blind, randomised, placebo-controlled pilot study which 24 individuals with metabolic syndrome were randomised filtrate (FFT) from lean healthy donor (n = 12) or placebo 12). The primary outcome, change metabolism, secondary...
Background The increasing interest to perform and investigate the efficacy of fecal microbiota transplantation (FMT) has generated an urge for feasible donor screening. We report our experience with stool recruitment, screening, follow-up, associated costs in context clinical FMT trials. Methods Potential donors, aged between 18−65 years, underwent a stepwise screening process starting extensive questionnaire followed by feces blood investigations. When eligible, donors were rescreened MDROs...
BACKGROUND: The microbiota-derived short chain fatty acid butyrate has been shown to lower blood pressure (BP) in rodent studies. Nonetheless, the net effect of on hypertension humans remains uncovered. In this study, for first time, we aimed determine oral BP patients with hypertension. METHODS: We performed a double-blind randomized placebo-controlled trial including 23 Antihypertensive medication was discontinued duration study washout period 4 weeks before starting intervention....
Objective Although gut dysbiosis is increasingly recognised as a pathophysiological component of metabolic syndrome (MetS), the role and mode action specific microbes in health remain elusive. Previously, we identified commensal butyrogenic Anaerobutyricum soehngenii to be associated with improved insulin sensitivity subjects MetS. In this proof-of-concept study, investigated potential therapeutic effects A. L2-7 on systemic responses duodenal transcriptome profiles individuals Design...
Fecal microbiota transplantation (FMT) is under investigation for several indications, including ulcerative colitis (UC). The clinical success of FMT depends partly on the engraftment viable bacteria. Because vast majority human gut consists anaerobes, currently used aerobic processing protocols donor stool may diminish bacterial viability transplanted material. This study assessed effect four techniques (i.e., anaerobic and aerobic, both direct after temporary cool storage) viability. By...
The intestinal gut microbiota is important for human metabolism and immunity can be influenced by many host factors. A recently emerged factor secreted microRNA (miRNA). Previously, it has been shown that miRNAs influence the growth of certain bacteria conversely, shifts in alter composition miRNAs. Here, we sought to further investigate interaction between use fecal transplantation (FMT). Subjects with metabolic syndrome received either an autologous (n = 4) or allogenic 14) FMT. Fecal...
Amino acids, metabolized by host cells as well commensal gut bacteria, have signaling effects on metabolism. Oral supplementation of the essential amino acid histidine has been shown to exert metabolic benefits. To investigate whether dietary aids glycemic control, we performed a case-controlled parallel clinical intervention study in participants with type 2 diabetes (T2D) and healthy controls. Participants received oral for seven weeks. After weeks supplementation, microbiome was depleted...
Background The spectrum of metabolic dysfunction-associated steatotic liver disease (MASLD) is highly prevalent, affecting 30% the world’s population, with a significant risk hepatic and cardiometabolic complications. Different stages MASLD are accompanied by distinct gut microbial profiles, several components have been implicated in pathophysiology. Indeed, earlier studies demonstrated that necroinflammation was reduced individuals after allogenic faecal microbiota transplantation (FMT)...
This randomized, double-blind, placebo-controlled trial investigated the impact of 14-day
Individuals with type 2 diabetes (T2D) show signs of low-grade inflammation, which is related to the development insulin resistance and beta-cell dysfunction. However, underlying triggers remain unknown. The gut microbiota a plausible source as it comprises pro-inflammatory bacteria, bacterial metabolites viruses, including (bacterio)phages. These prokaryotic viruses have been shown mediate inflammatory responses in gastrointestinal disease. Given differences phage populations healthy...
Fecal Microbiota Transplantation (FMT) has emerged as a potential modality for mitigating microbiome-associated diseases. Despite this potential, the precise causal pathways by which specific gut microbiota strains induce remission remain inadequately elucidated. In study, we aimed to discern impact of engraftment donor-infused on alterations in plasma metabolites, subsequently contributing amelioration clinical parameters involved subjects with metabolic syndrome (MetSyn) receiving an FMT....
Imidazole Propionate (ImP), a gut-derived metabolite from histidine, affects insulin signaling in mice and is elevated type 2 diabetes (T2D). However, the source of histidine role gut microbiota remain unclear. We conducted an intervention study humans, comparing ImP kinetics on high-fat diet with varying levels antibiotics, assessed healthy T2D subjects supplementation. Results show that dietary metabolized to ImP, antibiotic-induced suppression reducing mice. In contrast, oral...
Summary There is significant interest in altering the course of cardiometabolic disease development via gut microbiome. Nevertheless, highly abundant phage members -which impact bacteria- complex ecosystem remain understudied. Here, we characterized phageome changes associated with metabolic syndrome (MetS), a prevalent clinical condition preceding disease. MetS populations exhibited decreased richness and diversity, but larger inter-individual variation. These were enriched phages infecting...
The gut microbiota constitutes an important modulator of metabolic heath and unbalanced is increasingly recognized as risk factor for disorders, such obesity, insulin resistance type 2 diabetes. Seeking novel treatments to counteract disturbances in obese subjects, we explore whether delivering a protective commensal bacteria (Anaerobutyricum soehngenii L2-7) directly into the small intestine treatment-naïve subjects with syndrome would ameliorate glucose metabolism (NTR NL6630). We...
ABSTRACT Objective Bacteriophages (phages) are viruses of bacteria and have been shown to shape microbial communities. Previous studies that altering the microbiota through faecal transplantation (FMT) can improve insulin resistance in individuals with metabolic syndrome (MetSyn). Interestingly, similar effects were observed diet-induced obese mice after a virome (FVT), raising question whether phages healthy donor glucose metabolism MetSyn as well. Design We performed double-blind,...
Objective: The microbiota-derived short chain fatty acid (SCFA) butyrate has been shown to modulate blood pressure (BP) in rodent studies, while humans, plasma levels have associated with BP. Nonetheless the net effect of on hypertension humans remains uncovered. In this study, we aimed determine oral BP hypertensive patients. Design and method: We performed a double-blind randomized placebo-controlled trial including 23 patients grade I hypertension. Antihypertensive medication was...