A5056961843- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Botanical Research and Applications
- Chromosomal and Genetic Variations
- Plant Diversity and Evolution
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Antioxidant Activity and Oxidative Stress
- Plant biochemistry and biosynthesis
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Insect symbiosis and bacterial influences
- Advanced biosensing and bioanalysis techniques
- Genetic Mapping and Diversity in Plants and Animals
- Banana Cultivation and Research
Weizmann Institute of Science
2014-2023
Hebrew University of Jerusalem
2012-2023
Summary Current breeding relies mostly on random mutagenesis and recombination to generate novel genetic variation. However, targeted genome editing is becoming an increasingly important tool for precise plant breeding. Using the CRISPR ‐Cas system combined with bean yellow dwarf virus rolling circle replicon, we optimized a method gene replacement in tomato. The carotenoid isomerase ( CRTISO ) phytoene synthase 1 PSY genes from biosynthesis pathway were chosen as targets due their easily...
Abstract Homologous recombination (HR) between parental chromosomes occurs stochastically. Here, we report on targeted homologous upon somatic induction of DNA double-strand breaks (DSBs) via CRISPR-Cas9. We demonstrate this a visual and molecular assay whereby DSB two alleles carrying different mutations in the PHYTOENE SYNTHASE ( PSY1 ) gene results yellow fruits with wild type red sectors forming HR-mediated repair. also show that heterozygote plants containing one psy1 allele immune...
Tomato ( Solanum lycopersicum ) fruit accumulate the red carotenoid pigment lycopene. The recessive mutation yellow-flesh (locus r in tomato eliminates carotenoids by disrupting activity of fruit-specific phytoene synthase (PSY1), first committed step biosynthesis pathway. Fruits tangerine t appear orange due to accumulation 7,9,7′,9′-tetra- cis -lycopene (prolycopene) as a result – trans isomerase. It was established 60 y ago that is epistatic . This uncharacteristic epistasis interaction...
DNA double-stranded breaks (DSBs) generated by the Cas9 nuclease are commonly repaired via nonhomologous end-joining (NHEJ) or homologous recombination (HR). However, little is known about unrepaired DSBs and type of damage they trigger in plants. We designed an assay that detects loss heterozygosity (LOH) somatic cells, enabling study a broad range DSB-induced genomic events. The system relies on mapped phenotypic marker which produces light purple color (betalain pigment) all plant...
Homologous recombination (HR) in somatic cells is not as well understood meiotic and thought to be rare. In a previous study, we showed that Inter-Homologous Somatic Recombination (IHSR) can achieved by targeted induction of DNA double-strand breaks (DSBs). Here, designed novel IHSR assay investigate this phenomenon greater depth. We utilized F1 hybrids from divergent parental lines, each with different mutation at the Carotenoid isomerase (CRTISO) locus. events, namely crossover or gene...
Tomato (Solanum lycopersicum) is a prominent fruit with rich genetic resources for crop improvement. By using phenotype-guided screen of over 7900 tomato accessions from around the world, we identified new associations complex traits such as weight and total soluble solids (Brix). Here, present phenotypic data several years trials. To illustrate power this dataset use two case studies. First, evaluation color revealed allelic variation in phytoene synthase 1 that resulted differently colored...
Homologous recombination (HR) typically occurs during meiosis between homologs, at a few unplanned locations along the chromosomes. In this study, we tested whether targeted homologous chromosomes can be achieved via Clustered Regulatory Interspaced Short Palindromic Repeat associated protein Cas9 (CRISPR-Cas9)-induced DNA double-strand break (DSB) repair in Arabidopsis thaliana. Our experimental system includes targets for DSB induction euchromatic and heterochromatic genomic regions of...
Abstract The fate of DNA double-strand breaks (DSBs) generated by the Cas9 nuclease has been thoroughly studied. Repair via non-homologous end-joining (NHEJ) or homologous recombination (HR) is common outcome. However, little known about unrepaired DSBs and type damage they can trigger in plants. In this work, we designed a new assay that detects loss heterozygosity (LOH) somatic cells, enabling study broad range DSB-induced genomic events. system relies on mapped phenotypic marker which...