We previously demonstrated that mesenchymal stem cells (MSCs) ameliorated experimental autoimmune uveoretinitis (EAU) in rats. Recently, MSC-derived exosomes (MSC-Exo) were thought to carry functions of MSCs. In this study, we tested the effect local administration human MSC-Exo on established EAU same species. Rats with induced by immunization interphotoreceptor retinol-binding protein 1177-1191 peptide treated periocular injections increasing doses starting at disease onset for 7...

Although accumulated evidence supports the notion that mesenchymal stem cells (MSCs) act in a paracrine manner, mechanisms are still not fully understood. Recently, MSC-derived exosomes (MSC-Exos), type of microvesicle released from MSCs, were thought to carry functional proteins and RNAs recipient play therapeutic roles. In present study, we intravitreally injected MSCs derived either mouse adipose tissue or human umbilical cord, their observe compare functions model laser-induced retinal...

Mesenchymal stem cells (MSCs) exhibit a potent immunomodulatory capacity and have been applied to treat diseases such as graft versus host disease severe autoimmune diseases. However, the mechanism underlying their immunosuppressive effect is not yet completely understood. Here, we investigated role of CD73/adenosine pathway in immune modulation by MSCs using mouse model experimental uveitis (EAU). Moreover, examined vitro modulatory mediated through human T cells. We found that severity EAU...

This prospective cohort study aimed to assess the influence of tear film stability on corneal refractive power measurement and surgical planning in cataract patients. Participants were divided into instability (tear level 2) control (level 0-1) groups based Keratograph 5M results. Using IOL Master 700, two consecutive measurements obtained with a 10-min interval. Parameters including standard keratometry (Kf, Ks K), keratometric astigmatism (KCA), total (TKf, TKs TK) (TCA) recorded. was...

Based on the complex composition of kitchen waste, zero-valent iron addition and mixing substrate were proposed to enhance hydrolysis acidification waste. The results showed that ethanol acetic acid mainly products accounting for more than 90% total production. experimental group with a ratio 50% had highest efficiency during stage without ZVI addition. In general, system higher production TVFA was added dosage ZVI. reached level at same time 10 g/L Compared wet anaerobic fermentation, dry...

Abstract Background Autoimmune uveitis is an inflammatory disease triggered by aberrant immune response. Mesenchymal stem cell-derived small extracellular vesicles (MSC-sEVs) are emerging as potential therapeutic agents for this condition. CD73, ectoenzyme present on MSC-sEVs, involved in mitigating inflammation converting adenosine monophosphate into adenosine. We hypothesize that the inhibitory effect of MSC-sEVs experimental autoimmune (EAU) could be partially attributed to surface...

A metabolomics method was established to analyze changes of intracellular metabolites and study the mechanism for enhancing polyhydroxyalkanoates production by halotolerant bacteria, Bacillus cereus strain HY-3, using acetic acid as carbon source. Maximum poly(3-hydroxybutyrate) (PHB) contents medium with 0.5 g/L 5.0 were 41.0 ± 0.415% 49.2 1.21%. Principal components analysis revealed clear metabolic differences in different growth stages concentrations According statistical analysis,...

Purpose: To investigate the 3-months outcomes of patients who underwent intraoperative intravitreal injection Ozurdex for proliferative diabetic retinopathy (PDR).Methods: This is a prospective randomized controlled clinical trial (ChiCTR2100043399).Seventy-one with PDR had indications surgery without history within 3 months preoperatively were enrolled.Patients randomly divided into three groups based on medicine injected intraoperatively: Ozurdex, Conbercept, and Control group.The primary...

Abstract A retrospective cohort study was conducted to observe the correction effect of Toric intraocular lens (IOL) implantation in cataract eyes with specific types irregular corneal astigmatism. Thirty-four either "asymmetric bow-tie" pattern (Type I) or "angled II) were included. Corneal topography assessed using Pentacam HR, and changes preoperative astigmatism, visual acuity, manifest refraction, objective quality measured compared. The average uncorrected distance acuity improved...

To evaluate therapeutic outcomes of human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) treatment in patients with refractory uveitis.A retrospective and noncomparative review was performed on four uveitis from December 2013 to 2017. HUC-MSCs were administered intravenously at a dose 1×106 cells/kg. Clinical response, relapse rate, change visual acuity, other metrics evaluated.All presented responses treatment, three males one female. The numbers attacks per year after the (0, 2,...

Abstract Purpose To assess the differences and similarities in corneal curvature obtained by two swept-source optical coherence tomography (SS-OCT) devices, Scheimpflug imaging system one ray tracing aberrometer patients with cataracts. Moreover, this study aimed to compare posterior (PK), total (TK) true net power (TNP) measurements among IOLMaster 700, CASIA2, Pentacam. Methods A of 200 eyes (116 female, 58%) were enrolled study, a mean age 65.9 ± 9.5 years. The flattest (Kf), steepest...

To identify the genetic defects in a Chinese family with achromatopsia.A 2.5-year-old boy, who displayed nystagmus, photophobia, and hyperopia since early infancy, was clinically evaluated. further confirm localize causative mutations this family, targeted region capture next-generation sequencing of candidate genes, such as CNGA3, CNGB3, GNAT2, PDE6C, PDE6H were performed using custom-made array.Slit-lamp examination showed no specific findings anterior segments. The optic discs maculae...

Purpose: We sought to evaluate the efficacy and safety of plasmin injection in capsular bag during cataract operation for prevention posterior capsule opacification. Methods: Thirty-seven anterior flaps taken from phacoemulsification surgery were immersed either 1 µg/mL (plasmin group, n = 27) or phosphate-buffered saline (control 10) 2 minutes photographed after fixation nuclear staining compare numbers residual lens epithelial cells. In animal experiments, solution was injected into...

Abstract PURPOSE: To compare the performance of Barrett toric calculator incorporated with measured posterior corneal astigmatism (PCA) derived from IOL Master 700 and Pentacam HR versus predicted PCA. METHODS: The residual using Barrette PCA, PCA were calculated preoperative keratometry intended axis modification. vector analysis was performed to calculate mean absolute prediction error (MAE), centroid percentage eyes a within ±0.50 D, ± 0.75 1.00 D. RESULTS : In 57 patients age 70.42±10.75...

To evaluate and compare the accuracy of iTrace CASIA2 in measuring postoperative orientation toric intraocular lens (IOL) without mydriasis.Tianjin Medical University Eye Hospital, Tianjin, China.Prospective cohort study.Patients with SN6AT IOLs implanted after cataract surgery were enrolled. 1 month surgery, IOL measured by non-mydriatic, semi-dark conditions. Then, axis was directly reviewed using slit-lamp under full mydriasis. Axis measurement differences between each 2 devices...

<title>Abstract</title> <bold>Background:</bold> Autoimmune uveitis is an inflammatory disease triggered by aberrant immune response. Mesenchymal stem cell-derived small extracellular vesicles (MSC-sEVs) are emerging as potential therapeutic agents for this condition. CD73, ectoenzyme present on MSC-sEVs, involved in mitigating inflammation converting adenosine monophosphate into adenosine. We hypothesize that the inhibitory effect of MSC-sEVs experimental autoimmune (EAU) could be partially...

Abstract Background We aimed to investigate the anatomical features of optical coherence tomography (OCT) and vitreous cytokine levels as predictors outcomes combined phacovitrectomy with intravitreal dexamethasone (DEX) implants for idiopathic epiretinal membrane (iERM) treatment. Methods A prospective, single-masked, randomized, controlled clinical trial included 48 eyes. They were randomly assigned in a 1:1 ratio undergo DEX group (combined ERM peeling Ozurdex implantation) control...

To assess and compare the variations agreements across different ocular biometric parameters using swept-source optical coherence tomography (SS-OCT) Scheimpflug in patients diagnosed with cataract.