A5100732833- Wnt/β-catenin signaling in development and cancer
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Biotin and Related Studies
- Advanced Proteomics Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Lipid metabolism and biosynthesis
- Pluripotent Stem Cells Research
- Heat shock proteins research
- Photoreceptor and optogenetics research
- Plant Molecular Biology Research
- Kruppel-like factors research
- Retinal Development and Disorders
- Connexins and lens biology
- Nicotinic Acetylcholine Receptors Study
- Extracellular vesicles in disease
- Connective tissue disorders research
- Receptor Mechanisms and Signaling
- Research in Cotton Cultivation
- Glutathione Transferases and Polymorphisms
- Photosynthetic Processes and Mechanisms
- Ion channel regulation and function
- Ocular Diseases and Behçet’s Syndrome
- Redox biology and oxidative stress
Qilu Hospital of Shandong University
2025
Northeast Agricultural University
2025
Xiangya Hospital Central South University
2022
Central South University
2022
University of California, Los Angeles
2020
Southwest University
2019
Beijing Chuiyangliu Hospital
2017
Henan Normal University
2004-2016
Shandong University
2015
Capital Institute of Pediatrics
2013
We previously demonstrated that mesenchymal stem cells (MSCs) ameliorated experimental autoimmune uveoretinitis (EAU) in rats. Recently, MSC-derived exosomes (MSC-Exo) were thought to carry functions of MSCs. In this study, we tested the effect local administration human MSC-Exo on established EAU same species. Rats with induced by immunization interphotoreceptor retinol-binding protein 1177-1191 peptide treated periocular injections increasing doses starting at disease onset for 7...
Apolipoprotein B (apoB) is the major protein component of atherogenic lipoproteins hepatic origin. In HepG2 cells, standard cell culture model human lipoprotein metabolism, there a limited availability core lipids in endoplasmic reticulum for association with nascent apoB. Under these conditions, apoB partially translocated, interacts cytosolic Hsp70, and undergoes rapid degradation. We show that increasing expression Hsp70 cells promotes addition, polyubiquitinated its degradation both...
Auxin-dependent cell expansion is crucial for initiation of fiber cells in cotton (Gossypium hirsutum), which ultimately determines yield and quality. However, the regulation this process far from being well understood. In study, we demonstrate an antagonistic effect between cytokinin (CK) auxin on initiation. vitro planta experiments indicate that enhanced CK levels can reduce accumulation ovule integument, may account defects fiberless mutant xu142fl. turn, supplementation with recover...
When lipid synthesis is limited in HepG2 cells, apoprotein B100 (apoB100) not secreted but rapidly degraded by the ubiquitin-proteasome pathway. To investigate apoB100 biosynthesis and secretion further, physical functional states of destined for either degradation or lipoprotein assembly were studied under conditions which synthesis, proteasomal activity, microsomal triglyceride transfer protein (MTP) lipid-transfer activity varied. Cells pretreated with a inhibitor (which remained cells...
WNTs are secreted extracellular signaling molecules that transduce their signals by binding to G protein-coupled receptors of the frizzled (FZD) family. They control diverse developmental processes, such as cell fate specification, proliferation, differentiation, and apoptosis. Although WNT has been shown be essential for development ovary, its mechanistic role in folliculogenesis within adult ovary not studied extensively. Therefore, objective this study was investigate regulation function...
WNTs are extracellular signaling molecules that exert their actions through receptors of the frizzled (FZD) family. Previous work indicated WNT2 regulates cell proliferation in mouse granulosa cells acting CTNNB1 (beta-catenin), a key component canonical WNT signaling. In other cells, has been shown to regulate expression connexin43 (CX43), gap junction protein, as well assembly. Since previous demonstrated CX43 is also essential ovarian follicle development, objective this study was...
We have identified two yeast genes with similarity to a human cDNA encoding acyl-coenzyme A:cholesterol acyltransferase (ACAT). Deletion of both results in viable cell undetectable esterified sterol (Yang, H., Bard, M., Bruner, D. A., Gleeson, Deckelbaum, R. J., Aljinovic, G., Pohl, T., Rothstein, R., and Sturley, S. L. (1996) Science 272, 1353-1356). Here, we expressed the double mutant, resulting high level production ACAT protein, but low vivo esterification ergosterol, predominant...
Melanosomes are motile, light-absorbing organelles that present in pigment cells of the skin and eye. It has been proposed melanosome localization, both melanocytes retinal epithelium (RPE), involves capture from microtubule motors by an unconventional myosin, which dynamically tethers melanosomes to actin filaments. Recent studies with have questioned this cooperative model. Here, we test model RPE imaging associated labeled filaments microtubules, investigating roles different motor...
In the human hepatic cell line, HepG2, apolipoprotein B100 (apoB100) degradation is increased by inhibiting lipid transfer mediated microsomal triglyceride protein (MTP) and predominantly accomplished ubiquitin−proteasome pathway. current study, we determined whether this degradative pathway was restricted to HepG2 cells or of more general importance in apoB100 metabolism. Rat hepatoma McArdle RH7777 (McA), compared cells, secrete a large fraction associated with VLDL particles, as does...
Background Fanconi anemia is a severe congenital disorder associated with mutations in cluster of genes responsible for DNA repair. Arriving at an accurate and timely diagnosis can be difficult cases atypical clinical features. It very important to increase the rate such setting. The purpose this study explore children Methods Six features were enrolled study, their recorded, FANCA gene transcription was assessed by RT-PCR, ubiquitination FANCD2 protein analyzed using sequencing western...
Flowers are the main sexual reproductive organs in plants. The shapes, colours and scents of corolla plant flowers involved attracting insect pollinators increasing success. process senescence was investigated Ipomoea purpurea (Convolvulaceae) this study. In research methods anatomy, cytology, cell chemistry molecular biology were used. results showed that at flowering stage cells already began to show distortion, chromatin condensation, mitochondrial membrane degradation tonoplast...
WNTs are secreted extracellular signaling molecules that exert their effects by binding to G protein-coupled receptors of the frizzled (FZD) family. WNT has been found influence cell proliferation, differentiation, survival, apoptosis, polarity, and migration. In granulosa cells, our recent evidence indicates WNT2 signals through FZD9 receptor increase proliferation. other shown regulate expression gap junction protein, connexin43 (Cx43). Previous work from laboratories had demonstrated Cx43...