A5102982092- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Retinal Development and Disorders
- Kruppel-like factors research
- Connexins and lens biology
- Ocular Diseases and Behçet’s Syndrome
- Connective Tissue Growth Factor Research
- Extracellular vesicles in disease
- Endoplasmic Reticulum Stress and Disease
- Corneal Surgery and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cancer, Hypoxia, and Metabolism
- Wnt/β-catenin signaling in development and cancer
- Phytochemical and Pharmacological Studies
- Hops Chemistry and Applications
- Advanced Glycation End Products research
- Systemic Lupus Erythematosus Research
- Neurological Disease Mechanisms and Treatments
- RNA modifications and cancer
- RNA Research and Splicing
- Intraocular Surgery and Lenses
- Angiogenesis and VEGF in Cancer
- Corneal surgery and disorders
- Tendon Structure and Treatment
- Ocular Surface and Contact Lens
Tianjin Medical University
2015-2025
Tianjin Medical University Eye Hospital
2015-2025
Central South University
2021-2023
Xiangya Hospital Central South University
2021-2023
University of Oklahoma Health Sciences Center
2019-2022
Shanghai Jiao Tong University
2020
Binzhou University
2019
Binzhou Medical University
2019
Jilin University
2017
Calvary Mater Newcastle Hospital
2010
We previously demonstrated that mesenchymal stem cells (MSCs) ameliorated experimental autoimmune uveoretinitis (EAU) in rats. Recently, MSC-derived exosomes (MSC-Exo) were thought to carry functions of MSCs. In this study, we tested the effect local administration human MSC-Exo on established EAU same species. Rats with induced by immunization interphotoreceptor retinol-binding protein 1177-1191 peptide treated periocular injections increasing doses starting at disease onset for 7...
Aims Oxidative stress and apoptosis are among the earliest lesions of diabetic retinopathy. This study sought to examine anti-oxidative anti-apoptotic effects α-melanocyte-stimulating hormone (α-MSH) in early retinas explore underlying mechanisms retinal vascular endothelial cells. Methods Sprague-Dawley rats were injected intravenously with streptozocin induce diabetes. The intravitreally α-MSH or saline. At week 5 after diabetes, analyzed for reactive oxygen species (ROS) gene expression....
Abstract Objective Retinal degenerative diseases remain the dominant causes of blindness worldwide, and cell replacement is viewed as a promising therapeutic direction. However, resources seed cells are hard to obtain. To further explore this approach, human embryonic stem extracellular vesicles (hESEVs) were extracted from (hESCs) inspect its effect possible mechanism on retinal Müller function. Methods hESEVs by multi-step differential centrifugation, whose morphologies specific biomarkers...
Deficiency of endothelial progenitor cells, including colony-forming cells (ECFCs) and circulating angiogenic (CACs), plays an important role in retinal vascular degeneration diabetic retinopathy (DR). Fenofibrate, agonist peroxisome proliferator–activated receptor α (PPARα), has shown therapeutic effects on DR both patients animal models. However, the function PPARα ECFC/CACs not been defined. In this study, we determined regulation ECFC/CAC by PPARα. As flow cytometry Seahorse analysis,...
Background: Many studies have confirmed that high myopia is related to the prevalence of cataracts, which results from apoptosis lens epithelial cells (LECs) due endoplasmic reticulum stress. Krüppel-like factor 6 (KLF6) a tumor suppressor involved in regulation cell proliferation and apoptosis. Purpose: In this study, our purpose was find relationship between KLF6-induced LECs ATF4 (activating transcription 4)-ATF3 3)-CHOP (C/EBP homologous protein) signaling pathway. Methods: KLF6, ATF4,...
It was reported previously that circulation levels of kallistatin, an endogenous Wnt signaling inhibitor, are increased in patients with diabetes. The current study conducted to determine the role kallistatin delayed wound healing diabetic corneas. Immunostaining and Western blot analysis showed were upregulated corneas from humans rodents In murine corneal models, canonical activated nondiabetic suppressed corneas, correlating healing. Transgenic expression activation cornea Local...
Purpose.: We investigated the potential of human parthenogenetic embryonic stem cells (hPESCs) to differentiate into RPE cells, and identified development-regulating microRNAs (miRNAs). Methods.: were derived from hPESCs. The expression markers miRNA profiles during differentiation studied by immunocytochemistry, real-time RT-PCR, array at three time points. Human fetal (hfRPE) also analyzed. target genes candidate miRNAs then validated. Results.: hPESC-derived exhibited similar morphology...
Abstract A proportion of previously defined benign variants or uncertain significance in humans, which are challenging to identify, may induce an abnormal splicing process. An increasing number methods have been developed predict variants, but their performance has not completely evaluated using independent benchmarks. Here, we manually sourced ∼50 000 positive/negative from > 8000 studies and selected the evaluate prediction methods. These showed different performances recognizing...
Abstract Background This observational, prospective, analytical, and cross-sectional study was designed to systematically analyze ocular biometric parameters in patients with nuclear cataract exhibiting hardness grade ≥ 3 according the Emery-Little classification under slit-lamp examination who scheduled for phacoemulsification. Ocular measurements were acquired using IOL Master 700, a swept-source optical coherence tomography (SS-OCT) device. Results Age negatively correlated axial length...
Endothelial progenitor cells (EPCs) contribute to blood vessel formation. Canonical Wnt signaling plays an important role in physiological and pathological angiogenesis EPC fate regulation. However, the mechanism for regulate neovascularization (NV) has not been clearly defined. Here, we showed that very low-density lipoprotein receptor knockout (Vldlr -/- ) mice, a model of ocular NV induced by overactivation, have increased numbers bone marrow, blood, retina, as well elevated mitochondrial...
Microglial activation and subsequent pathological neuroinflammation contribute to diabetic retinopathy (DR). However, the underlying mechanisms of microgliosis, means effectively suppress remain incompletely understood. Peroxisome proliferator-activated receptor alpha (PPARα) is a transcription factor that regulates lipid metabolism. The present study aimed determine if PPARα affects microgliosis in DR. In global Pparα mice, retinal microglia exhibited decreased structural complexity...
Purpose: To observe the protective effects and underlying mechanisms of dl-3-n-butylphthalide (NBP) against H2O2-induced oxidative damage in retinal Müller cells.Methods: Cultured human cell line (MIO-M1line) were exposed to H2O2 for 2 hours. Cell survival was evaluated by Calcein AM viability assay. Dichlorofluorescein diacetate (DCFDA) + endoplasmic reticulum (ER) red fluorescent probe (ER-Tracker Red) staining used expression level reactive oxygen species (ROS) ER cells. Mitochondrial...
Etiology and pathogenesis of age-related cataract is not entirely clear till now. Senescence marker protein 30 (SMP30) a newly discovered anti-aging factor, which plays an important role in preventing apoptosis reducing oxidative stress damage. Mitochondria are located at the intersection key cellular pathways, such as energy substrate metabolism, reactive oxygen species (ROS) production apoptosis. Oxidative induced by 4-hydroxynonenal (4-HNE) closely related to neurodegenerative diseases...
To test the therapeutic effects of delayed treatment mesenchymal stem cells (MSCs) in recurrent experimental autoimmune uveitis (rEAU).The efficacy different regimens MSC administration rEAU were tested by evaluation clinical and pathological intraocular inflammation, as well retinal structural functional integrity using optical coherence tomography (OCT) electroretinogram (ERG). The sections also immunostained with antibodies to glial fibrillary acidic protein (GFAP) rhodopsin (RHO).Delayed...
B7-H4, as a member of the B7 superfamily, was overexpressed in various types cancers. However, effects B7-H4 on aggressiveness HCC and underlying mechanisms have not yet been fully explored. For this purpose, expression detected by Flow cytometry Western blotting, it highly expressed several cell lines but normal LO2 line. Knockdown induced cells apoptosis flow colony formation assays increased apoptosis-related proteins, including survivin, cleaved caspase-3, caspase-7, Bax, while...
Functional analysis of single Toll-like receptors (TLRs) in vivo is necessary to understand how they shape the ocular inflammation involved uveitis. In this study we explored role and mechanisms TLR-2 agonists on autoreactive T helper type 17 (Th17) response experimental autoimmune uveitis (EAU). Treatment by peptidoglycan (PGN), a specific agonist, remarkably increased mRNA levels Th17-lineage genes interleukin (IL)-17A, IL-21 RAR-related orphan receptor (ROR)γt promoted antigen-specific...
Genomic variants outside of the canonical splicing site (±2) may generate abnormal mRNA splicing, which are defined as non-canonical (NCSVs). However, clinical interpretation NCSVs in neurodevelopmental disorders (NDDs) is largely unknown.