A5040824194- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Ocular Diseases and Behçet’s Syndrome
- Immunotherapy and Immune Responses
- Systemic Lupus Erythematosus Research
- Adenosine and Purinergic Signaling
- Cytomegalovirus and herpesvirus research
- Neuroinflammation and Neurodegeneration Mechanisms
- Psoriasis: Treatment and Pathogenesis
- Monoclonal and Polyclonal Antibodies Research
- interferon and immune responses
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Ocular Surface and Contact Lens
- Immunodeficiency and Autoimmune Disorders
- Mast cells and histamine
- Immune cells in cancer
- Kidney Stones and Urolithiasis Treatments
- Salivary Gland Disorders and Functions
- Cell Adhesion Molecules Research
- Microscopic Colitis
- Chronic Lymphocytic Leukemia Research
- Metabolism and Genetic Disorders
- Muscle and Compartmental Disorders
- Advanced Glycation End Products research
Doheny Eye Institute
2016-2025
University of California, Los Angeles
2015-2025
Weichai Power (China)
2025
Jilin University
2016-2023
National Health and Family Planning Commission
2017
First Hospital of Jilin University
2016
University of Southern California
2008-2015
UCLA Health
2015
University of Louisville
2002-2009
Shanghai Children's Hospital
2005-2006
Encephalitogenic T cells that mediate experimental autoimmune encephalomyelitis (EAE) are commonly assumed to be exclusively CD4+, but formal proof is still lacking. In this study, we report synthetic peptides 35-55 from myelin oligodendrocyte glycoprotein (pMOG(35-55)) consistently activate a high proportion of CD8+ alphabetaTCR+ encephalitogenic in C57BL/6 (B6) mice. The potential MOG-specific was established by adoptive transfer CD8-enriched cells. These induced much more severe and...
Author(s): Tezel, Gulgun; Fourth ARVO/Pfizer Ophthalmics Research Institute Conference Working Group
purpose. Evidence supports the immune system activity accompanying glaucomatous neurodegeneration. This study aimed to determine in vitro effects of reactive oxygen species (ROS) on phenotype and antigen-presenting function retina optic nerve head glia. methods. Cultures rat glia were treated with a mixture ROS-generating compounds for 24 48 hours. Pretreated glial cells then coincubated syngeneic CD4+ T ROS generation cell viability assessed use dihydroethidium calcein assays, respectively....
This study explores the usage of T cell antigen receptor (TCR) beta chain elements in Lewis rats with experimentally induced allergic encephalomyelitis (EAE). TCRs from 15 different clones and hybridomas derived animals immunized myelin basic protein (MBP), all having specificity for 21-mer encephalitogenic fragment MBP 68-88, utilized V 8.2. In addition, there was a marked conservation first two amino acid residues junctional complementarity determining region 3 (CDR3) associated 8.2...
The aims of this study were to determine whether IL-17(+) T cells present in CD4 and CD8 interphotoreceptor retinoid-binding protein (IRBP)-specific the role antigen-specific nonspecific pathogenesis experimental autoimmune uveitis (EAU).B6 mice immunized with uveitogenic peptide IRBP1-20. In vivo-primed separated stimulated immunizing peptide. Intracellular expression IFN-gamma IL-17 by was assessed, pathogenic activity activated determined.A subset autoreactive IRBP-specific expressed...
Abstract We show that in vitro activation of interphotoreceptor retinoid-binding protein (IRBP)-specific T cells from C57BL/6 mice immunized with an uveitogenic IRBP peptide (IRBP1–20) under TH17-polarizing conditions is associated increased expansion expressing the γδ TCR. also highly purified αβ or IRBP1–20 produced only small amounts IL-17 after exposure to immunizing Ag vitro, whereas a mixture same greatly IL-17. IRBP-induced IRBP-immunized TCR-δ−/− on genetic background significantly...
Previous studies have shown that IL-17 is a strong proinflammatory cytokine and IL-17-producing autoreactive T cells play major role in the pathogenesis of autoimmune diseases. In previous study, we showed injection experimental uveitis-susceptible mice with anti-IL-17 Abs blocked subsequent disease development. To determine whether administration to Lewis rats B10RIII injected disease-inducing peptides enhanced susceptibility, recipient animals various doses human rIL-17 (hIL-17)....
Short chain fatty acids (SCFAs) are produced by gut microbiota as fermentation products of digestion-resistant oligosaccharides and fibers. Their primary roles functioning major energy sources for colon cells assisting in homeostasis immunomodulation. Recent evidence suggests that they affect various organs both at cellular molecular levels, regulate functions distance sites including gene expression, cell proliferation, differentiation, apoptosis inflammation. In this study, we examined...
Purpose: To investigate the effects and mechanisms of fenofibrate, a synthetic ligand peroxisome proliferator-activated receptor α (PPAR-α), on autoimmune dacryoadenitis in mouse model Sjögren syndrome (SS) dry eye. Methods: Male nonobese diabetic (NOD) mice were fed chow with or without 0.03% fenofibrate for 8 weeks, clinical scores determined by assessing tear secretion, fluorescein, hematoxylin eosin staining. Intracellular IFN-γ, IL-17, Foxp3 CD4+ T cells measured flow cytometry. The...
T cell-to-T cell Ag presentation is increasingly attracting attention. In this study, we demonstrated that active CD4+ (aT) cells with uptake of OVA-pulsed dendritic cell-derived exosome (EXO(OVA)) express exosomal peptide/MHC class I and costimulatory molecules. These EXO(OVA)-uptaken (targeted) aT can stimulate CD8+ proliferation differentiation into central memory CTLs induce more efficient in vivo antitumor immunity long-term responses than cells. They also counteract CD4+25+ regulatory...
To determine how the activation of γδ T cells affects generation uveitogenic αβ and development experimental autoimmune uveitis (EAU).γδ were isolated from B6 mice immunized with peptide IRBP(1-20) TCR-δ(-/-) mice. Resting prepared by culture separated in cytokine-free medium for 3 to 5 days, when they showed downregulation CD69 expression. Activated incubating resting anti-γδ TCR (GL3) 2 days. Responder cocultured immunizing antigen antigen-presenting cells. The numbers antigen-specific...
Abstract We have previously reported that IL-17+ interphotoreceptor retinoid-binding protein (IRBP) 161–180-specific T cells a strong pathogenic effect in experimental autoimmune uveitis (EAU) induced B10RIII mice; however, this activity is not solely attributable to the major cytokine, IL-17, produced by these cells. To determine whether other cytokines Th17 show stronger association with their activity, we studied role of IL-22 EAU. one Our results showed administration small doses...
We have previously reported that, depending on their activation status, mouse γδ T cells can either enhance or inhibit the activity of IL-17(+) autoreactive in experimental autoimmune uveitis. In this study, we showed that naive C57BL/6 (B6) do not express IL-23R, whereas immunized mice, it is expressed >50% cells. vitro studies IL-23R expression modulated by state activation, as weakly activated but highly did not. Functional IL-23R(+) had strongest suppressive effect cells, and was...
Abstract It is clear that dendritic cells (DCs) are essential for priming of T cell responses against tumors. However, the distinct roles DC subsets play in regulation vivo largely undefined. In this study, we investigated capacity OVA-presenting CD4−8−, CD4+8−, or CD4−8+ DCs (OVA-pulsed (DCOVA)) stimulation OVA-specific responses. Our data show each subset stimulated proliferation allogeneic and autologous CD4+ CD8+ vitro, but CD4−8− did so only weakly. Both CD4+8− DCOVA induced strong...
γδ T cells can either enhance or inhibit an adaptive immune response, but the mechanisms involved are not fully understood. Given that CD73 is main enzyme responsible for conversion of AMP into immunosuppressive molecule adenosine, we investigated its role in regulatory function experimental autoimmune uveitis (EAU). We found expressed different amounts during stages EAU and low expression on correlated with enhanced Th17 response-promoting activity. Functional comparison CD73-deficient...
Abstract Adenosine is a key endogenous signaling molecule that regulates wide range of physiological functions, including immune system function and inflammation. Studies have shown adenosine receptor (AR) agonists can be either anti-inflammatory or proinflammatory in responses inflammation, the clarification mechanisms causing these opposing effects should provide better guide for therapeutic intervention. Whereas previous studies mostly examined AR on Th1-type responses, this study, we...
Pathogenic T helper (T h )17 cells are key mediators of autoimmune diseases such as uveitis and its animal model, experimental (EAU). However, the contribution microRNAs (miRs) to intrinsic control pathogenic 17 in EAU remains largely unknown. Here, we have reported that miR‐223‐3p was significantly up‐regulated interphotoreceptor retinoid‐binding protein‐specific cells, expression enhanced by IL‐23‐signal transducer activator transcription 3 signaling. Knockdown decreased pathogenicity a...
Hydraulic mechanical continuously variable transmission (HMCVT) is widely used in powerful tractors due to its excellent performance. This paper aims find universal methods for analyzing and optimizing the efficiency of HMCVT. The energy improvement HMCVT important economy tractors. Firstly, by correctly HMCVT, during operation can be accurately calculated. Secondly, an improved NSGA-II genetic algorithm was adopted achieve dynamic optimization shifting points through parameter combination...