A5013540987- Ion channel regulation and function
- Cellular Mechanics and Interactions
- Cardiac electrophysiology and arrhythmias
- 3D Printing in Biomedical Research
- Cell Adhesion Molecules Research
- Ion Channels and Receptors
- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- Cardiomyopathy and Myosin Studies
- Tissue Engineering and Regenerative Medicine
- Analytical Chemistry and Sensors
- Neuroscience and Neural Engineering
- Nitric Oxide and Endothelin Effects
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Liver physiology and pathology
- Cardiac Ischemia and Reperfusion
- Neuroscience and Neuropharmacology Research
- Spaceflight effects on biology
- Cardiovascular Effects of Exercise
- Erythrocyte Function and Pathophysiology
- Congenital heart defects research
- Organ Transplantation Techniques and Outcomes
- Sperm and Testicular Function
- Protease and Inhibitor Mechanisms
Okayama University
2016-2025
National Hospital Organization Kochi National Hospital
2004-2022
Oita University
2018
Doshisha University
2018
National Institute of Advanced Industrial Science and Technology
2018
Okayama Prefecture
2015
Menicon (Japan)
2010
National Institute for Basic Biology
2010
Nagoya University
1999-2008
Toyohashi Municipal Hospital
2007
Alternative models of cell mechanics depict the living as a simple mechanical continuum, porous filament gel, tensed cortical membrane, or tensegrity network that maintains stabilizing prestress through incorporation discrete structural elements bear compression. Real-time microscopic analysis cells containing GFP-labeled microtubules and associated mitochondria revealed behave like structures composed an interconnected actin microfilaments when stresses are applied to surface integrin...
Transient receptor potential channels have recently been implicated in physiological functions a urogenital system. In this study, we investigated the role of transient vanilloid 4 (TRPV4) stretch sensing mechanism mouse primary urothelial cell cultures. The selective TRPV4 agonist, 4alpha-phorbol 12,13-didecanoate (4alpha-PDD) evoked Ca(2+) influx wild-type (WT) cells, but not TRPV4-deficient (TRPV4KO) cells. We established cell-stretch system to investigate stretch-evoked changes...
Endothelial cells are subjected to shear stresses by blood flow, normal pressure, and stretch vessel expansion. These forces known induce secretions of several vasoactive substances probably via internal calcium mobilization (R. F. Furchgott. Circ. Res. 53: 557-573, 1983; M. J. Peach, A. L. Loeb, H. Singer, Saye. Hypertension Dallas 7, Suppl. I: I-94-I-100, 1985). Here we report that stretching cellular membranes increased intracellular Ca2+ concentration ([Ca2+]i) in human umbilical...
We examined whether prostaglandin (PG) H2, as an endothelium-dependent contracting factor, or the disturbed production of endothelium-derived relaxing impairs relaxation and long-term inhibition nitric oxide (NO) synthesis aggravates atherosclerosis in hypercholesterolemic rabbits. Male New Zealand White rabbits were fed one following diets: (1) standard chow; (2) 2% cholesterol-supplemented (3) chow with 80 micrograms/mL N omega-nitro-L-arginine methylester (L-NAME), NO synthetase...
The present work was designed to elucidate the involvement of Ca(2+)-permeable stretch-activated (SA) channels in orienting response endothelial cells uniaxial cyclic stretch. Endothelial from human umbilical vein were cultured on an elastic silicone membrane and subjected stretch (120% length, 1 Hz). started change their morphology 15 min after onset stretch, > 90% oriented perpendicularly axis 2 h. Associated with response, cell elongation proceeded a slower rate. Both orientating...
Increased protein kinase C (PKC) activity has been implicated in the pathogenesis of diabetic retinopathy and nephropathy. However, role PKC neuropathy remains unclear. The present study was conducted to compare effect inhibition by a PKC-beta-selective inhibitor, LY333531 (LY), on nerve dysfunction with that an aldose reductase NZ-314 (NZ). Streptozotocin-induced rats were treated or without LY and/or NZ for 4 weeks, motor conduction velocity (MNCV), coefficient variation R-R interval...
Calcium-permeable, stretch-activated nonselective cation (SA Cat) channels mediate cellular responses to mechanical stimuli. However, genes encoding such have not been identified in eukaryotes. The yeast MID1 gene product (Mid1) is required for calcium influx the Saccharomyces cerevisiae . Functional expression of Mid1 Chinese hamster ovary cells conferred sensitivity stress that resulted increases both conductance and concentration cytosolic free calcium. These were dependent on presence...
Mechanical loading plays a vital role in maintaining bone architecture. The process by which osteoblasts convert mechanical signals into biochemical responses leading to remodeling is not fully understood. earliest cellular response detected mechanically stimulated an increase intracellular calcium concentration ([Ca(2+)](i)). In this study, we used the clonal mouse osteoblast cell line MC3T3-E1 show that uniaxial cyclic stretch induces: (1) immediate [Ca(2+)](i), and (2) phosphorylation of...
The heart has a dynamic compensatory mechanism for haemodynamic stress. However, the molecular details of how mechanical forces are transduced in unclear. Here we show that transient receptor potential, vanilloid family type 2 (TRPV2) cation channel is critical maintenance cardiac structure and function. Within 4 days eliminating TRPV2 from hearts adult mice, function declines severely, with disorganization intercalated discs support coupling neighbouring myocytes myocardial conduction...
Embryonic stem (ES) cells are exposed to fluid-mechanical forces, such as cyclic strain and shear stress, during the process of embryonic development but much remains be elucidated concerning role forces in ES cell differentiation. Here, we show that induces vascular smooth muscle (VSMC) differentiation murine cells. Flk-1-positive (Flk-1 + ) seeded on flexible silicone membranes were subjected controlled levels examined for changes proliferation expression various lineage markers. When...
The aim of this present study was to provide a scaffold as tool for the investigation effect mechanical stimulation on three-dimensionally cultured cells. For purpose, we developed an artificial self-assembling peptide (SPG-178) hydrogel scaffold. structural properties SPG-178 were confirmed by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and transmission electron microscopy (TEM). studied using rheology measurements. able form stable, transparent in neutral...
The genetic basis of hypoplastic left heart syndrome (HLHS) remains unknown, and the lack animal models to reconstitute cardiac maldevelopment has hampered study this disease. This investigated altered control transcriptional epigenetic programs that may affect development HLHS by using disease-specific induced pluripotent stem (iPS) cells. Cardiac progenitor cells (CPCs) were isolated from patients with congenital diseases generate patient-specific iPS Comparative gene expression analysis...
In recent years, research on organ-on-a-chip technology has been flourishing, particularly for drug screening and disease model development. Fibroblasts vascular endothelial cells engage in crosstalk through paracrine signaling direct cell-cell contact, which is essential the normal development function of heart. Therefore, to faithfully recapitulate cardiac function, it imperative incorporate fibroblasts into a heart-on-a-chip model. Here, we report human composed induced pluripotent stem...
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Microtubules are structural components of the cytoskeleton that determine cell shape, polarity, and motility in cooperation with actin filaments. In order to role microtubules alignment, human airway smooth muscle cells were exposed cyclic uniaxial stretch. Human cells, cultured on type I collagen-coated elastic silicone membranes, stretched uniaxially (20% strain, 30 cycles/min) for 2 h. The population which originally oriented randomly aligned near perpendicular stretch axis a...