A5041350214- Reproductive Biology and Fertility
- Reproductive System and Pregnancy
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Reproductive Physiology in Livestock
- Plant Molecular Biology Research
- Single-cell and spatial transcriptomics
- Ovarian function and disorders
- Chromosomal and Genetic Variations
- Genetics, Aging, and Longevity in Model Organisms
Heidelberg University
2022-2025
German Cancer Research Center
2022-2025
European Molecular Biology Laboratory
2022-2023
École Normale Supérieure - PSL
2023
The female reproductive tract (FRT) undergoes extensive remodeling during cycling. This recurrent and how it shapes organ-specific aging remains poorly explored. Using single-cell spatial transcriptomics, we systematically characterized morphological gene expression changes occurring in ovary, oviduct, uterus, cervix, vagina at each phase of the mouse estrous cycle, decidualization, into aging. These analyses reveal that fibroblasts play central-and highly organ-specific-roles FRT by...
The boundaries of topologically associating domains (TADs) are delimited by insulators and/or active promoters; however, how they initially established during embryogenesis remains unclear. Here, we examined this the first hours Drosophila embryogenesis. DNA-FISH confirms that intra-TAD pairwise proximity is zygotic genome activation (ZGA) but with extensive cell-to-cell heterogeneity. Most newly formed occupied combinations CTCF, BEAF-32, CP190. Depleting each insulator individually from...
Abstract In vitro fertilization efficiency is limited in part because a fraction of retrieved oocytes fails to fertilize. Accurately evaluating their quality could significantly improve efficiency, which would require better understanding how maturation may be disrupted. Here, we quantitatively investigate the interplay between superovulation and aging mouse paired granulosa cells using newly adapted experimental methodology. We test hypothesis that disrupts oocyte maturation, revealing key...
The female reproductive tract (FRT) undergoes extensive remodeling during each cycle, regulated by systemic changes in sex hormones. Whether this recurrent influences a specific organ’s aging trajectory is unknown. To address this, we systematically characterized at single-cell resolution the morphological and transcriptional that occur ovary, oviduct, uterus, cervix, vagina phase of mouse estrus decidualization, into aging. Transcriptional cell-to-cell communication networks cycle are...
SUMMARY The boundaries of Topologically-Associating Domains (TADs) are delimited by insulators and active promoters, however how they initially established during embryogenesis remains unclear. Here, we examined this the first hours Drosophila embryogenesis. DNA-FISH on individual embryos indicates that domains form zygotic genome activation (ZGA), but have extensive cell-to-cell heterogeneity compared to later stages. Most newly formed occupied combinations CTCF, BEAF- 32 and/or CP190....
Abstract In vitro fertilization has been developed to overcome reduced fertility, which is increasingly due a decline in reproductive cell quality during aging. Here, we quantitatively investigated the interplay between superovulation and aging mouse oocytes their paired granulosa cells using newly adapted isolation techniques. We tested hypothesis that disrupts oocyte maturation, revealing key intercellular communication pathways dysregulated by forced hormonal stimulation. further...