A5102140668- Electrospun Nanofibers in Biomedical Applications
- Mitochondrial Function and Pathology
- Genetic Neurodegenerative Diseases
- Tissue Engineering and Regenerative Medicine
- Genetics and Neurodevelopmental Disorders
- Silk-based biomaterials and applications
- Nanoparticles: synthesis and applications
- Cellular Mechanics and Interactions
- Bone Tissue Engineering Materials
- Neuroscience and Neuropharmacology Research
- Cerebrovascular and genetic disorders
- Advanced Neuroimaging Techniques and Applications
- RNA regulation and disease
- Pediatric health and respiratory diseases
- ATP Synthase and ATPases Research
- Metal and Thin Film Mechanics
- Nerve injury and regeneration
- Amyotrophic Lateral Sclerosis Research
- Force Microscopy Techniques and Applications
- RNA Research and Splicing
- Chemical Reactions and Isotopes
- Hearing, Cochlea, Tinnitus, Genetics
- Marine Ecology and Invasive Species
- Cerebrovascular and Carotid Artery Diseases
- Genomics and Rare Diseases
Sun Yat-sen University
2019-2025
Zhongshan Hospital
2019-2025
Fudan University
2019-2025
The First Affiliated Hospital, Sun Yat-sen University
2019-2025
Union Hospital
2010-2019
Huazhong University of Science and Technology
2010-2019
Engineering (Italy)
2015
National University of Singapore
2006-2015
Faculty (United Kingdom)
2015
Fabrication of nanofibrous scaffolds with well-defined architecture mimicking native extracellular matrix analog has significant potentials for many specific tissue engineering and organs regeneration applications. The fabrication aligned collagen by electrospinning was described in this study. structure vitro properties these were compared a random scaffold. All the first crosslinked glutaraldehyde vapor to enhance biostability keep initial nano-scale dimension intact. From culture rabbit...
Abstract Human erythrocytes or red blood cells (RBCs), which constitute 99% of cells, perform an important function oxygen transport and can be exposed to nanoparticles (NPs) entering into the human body during therapeutical applications involving such NPs. Hence, haemocompatibility Ag, Au, Pt NPs on RBCs is investigated. The parameters monitored include haemolysis, haemagglutination, erythrocyte sedimentation rate, membrane topography, lipid peroxidation. findings suggest that platinum gold...
Mussel inspired proteins have been demonstrated to serve as a versatile biologic adhesive with numerous applications. The present study illustrates the use of such (polydopamine) in fabrication functionalized bio-inspired nanomaterials capable both improving cell response and sustained delivery model probes. X-ray photoelectron spectroscopy analysis confirmed ability dopamine polymerize on surface plasma-treated, electrospun poly(ε-caprolactone) (PCL) fiber mats form polydopamine coating....
The crosslinking methods of collagen using glutaraldehyde (GTA) and 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) are frequently performed in biomedical applications, but both still have their own disadvantages, including the GTA cytotoxicity low degree EDC/NHS crosslinking. In this study, we incorporated polyamidoamine (PAMAM) dendrimer with surface amine groups into two aforementioned to improve biostability structural integrity collagen. Fifty micromolar...
The actin cytoskeleton in eukaryotic cells undergoes drastic rearrangement during mitosis. changes are most obvious the adherent where stress fibres disassembled and cortical network becomes more prominent with concomitant increase cell rigidity as round up enter While regulatory connections between early mitotic events apparent, mechanisms that govern these links not well understood. Here, we report LIMK1 LIMK2, downstream effectors of RhoA/ROCK, regulate centrosome integrity astral...
Considerable research has been focused on the utilization of electrospun ultrafine fibers to construct biomimetic scaffolds for tissue engineering applications. However, there is still a limited understanding effects fiber alignment stem cells-fibrous scaffold interactions and their role in deciding fate cells scaffolds. Here, we tracked real-time between mesenchymal (MSCs) aligned/non-aligned PCL-gelatin using time-lapse microscopy, investigated behaviour cell spreading, migration...
To identify transcriptome signatures underlying epileptogenesis in temporal lobe epilepsy (TLE).Robust rank aggregation analysis was used to integrate multiple microarrays rodent models of TLE and determine differentially expressed genes (DEGs) acute, latent, chronic stages. Functional annotation protein-protein interaction were performed explore the potential functions DEGs hub with highest intramodular connectivity. The association between hippocampal sclerosis/seizure frequency analyzed...
NOTCH2NLC-related neuronal intranuclear inclusion disease (NIID) is a neurodegenerative with characteristic white matter lesions (WMLs) visible on MRI. However, the distribution of WMLs and their clinical correlations remain poorly understood in NIID. This study aims to investigate spatial temporal brain patients We retrospectively evaluated diagnosed NIID Zhongshan Hospital, Fudan University. Detailed information, including retrospective MRI data, was collected. Spatial fluid-attenuated...
Abstract Background Fragile X-associated tremor/ataxia syndrome (FXTAS) is a neurodegenerative disorder caused by CGG repeat expansion of FMR1 gene. Both FXTAS and neuronal intranuclear inclusion disease (NIID) belong to polyglycine diseases present similar clinical, radiological, pathological features, making it difficult distinguish these diseases. Reversible encephalitis-like attacks are often observed in NIID. It unclear whether they presented can be used for differential diagnosis NIID...
Abstract Objective To evaluate the relationships between cognitive function and white matter hyperintensity volume (WMHV) in patients with silent cerebrovascular disease to investigate whether integrity or brain atrophy play a role this association. Methods Automated Fiber Quantification Voxel‐ based morphometry were used track identify of 20 well‐defined tracts measure gray (GMV). A linear regression model was applied for examining associations WMHV mediation analysis roles GMV influence on...
Objective To investigate the effects of Netrin-1 on angiogenesis in vitro and vivo. Methods We performed rat aortic ring assay vivo Matrigel plug to determine effect angiogenesis. Results 10 μg/L, 50 μg/L 100 stimulated microvessel sprouting from adventitia rings reached maximum at The showed orange color change if Nestrin-1 was positive; CD34 immunofluorescent staining vascular structures with hemachrome ( 53.4 ± 7. 3 ), which significantly higher than control 5. 8 0. 9 )Conclusions can...
To explore the genetic basis for a patient with syndromic hearing loss.Genomic DNA of was extracted, which 127 deafness-related genes were enriched chip. Following next generation sequencing, pathogenic loci in exonic regions analyzed through comparison against databases. Genotype her fetus suspected site determined by testing amniotic fluid sample. qPCR method applied to verify deletion large fragment.The proband diagnosed Waardenburg syndrome type 2, and had harbored novel heterozygous...
Mechanistic target of rapamycin (mTOR) pathway has been associated to various neurological diseases, including epilepsy. As a newly discovered mTOR disease, DEPDC5 (dishevelled, Egl-10, and pleckstrin domain-containing protein 5) gene related epilepsy covers range clinical phenotypes. Mutations have detected in genetic focal epilepsies, cortical dysplasia type Ⅱ, sporadic cases. It reported that hyperactivation signaling due the loss function contributes epileptogenesis, however more...