A5027651871- Hemodynamic Monitoring and Therapy
- Sepsis Diagnosis and Treatment
- Iron Metabolism and Disorders
- Immune Response and Inflammation
- Trauma and Emergency Care Studies
- Cardiac Arrest and Resuscitation
- Hemoglobinopathies and Related Disorders
- Cardiac, Anesthesia and Surgical Outcomes
- Anesthesia and Sedative Agents
- Non-Invasive Vital Sign Monitoring
- Respiratory Support and Mechanisms
- Pregnancy and preeclampsia studies
- Stress Responses and Cortisol
- Airway Management and Intubation Techniques
- Cardiovascular Issues in Pregnancy
- Pregnancy-related medical research
- Intensive Care Unit Cognitive Disorders
- Erythropoietin and Anemia Treatment
- Disaster Response and Management
- Preterm Birth and Chorioamnionitis
- Reproductive System and Pregnancy
- Anesthesia and Neurotoxicity Research
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Vagus Nerve Stimulation Research
- Genetic Neurodegenerative Diseases
Radboud University Nijmegen
2015-2025
Radboud University Medical Center
2015-2025
University Medical Center
2018-2024
Radboud Institute for Molecular Life Sciences
2018-2020
Dutch Research Council
2014
Pfizer (Netherlands)
2014
Noxxon Pharma (Germany)
2014
Institute of Infection and Immunity
2014
Exponent (United States)
2014
AM–Pharma (Netherlands)
2014
Significance Hitherto, both the autonomic nervous system and innate immune were regarded as systems that cannot be voluntarily influenced. The present study demonstrates that, through practicing techniques learned in a short-term training program, sympathetic can indeed Healthy volunteers exhibited profound increases release of epinephrine, which turn led to increased production anti-inflammatory mediators subsequent dampening proinflammatory cytokine response elicited by intravenous...
Objective: To determine gender differences in the innate immune response and vascular reactivity during human endotoxemia. Design: Clinical experimental study. Setting: University medical center intensive care research unit. Subjects: Fifteen female 15 male volunteers. Interventions: Intravenous injection of 2 ng/kg Escherichia coli lipopolysaccharide. Measurements Main Results: C-reactive protein, leukocytes, cytokines were measured at regular time intervals as indicators inflammation....
BackgroundHyperalgesia is a well recognized hallmark of disease. Pro-inflammatory cytokines have been suggested to be mainly responsible, but human data are scarce. Changes in pain threshold during systemic inflammation evoked by endotoxemia, were evaluated with three quantitative sensory testing methods. Methods and ResultsPressure thresholds, electrical thresholds tolerance the cold pressor test measured before 2 hours after intravenous administration ng/kg purified E. coli endotoxin 27...
Although endothelial dysfunction is central to the pathogenesis of sepsis, no specific and clinically applicable marker for currently available. Endocan, a proteoglycan excreted by cells in response inflammatory cytokines, may serve as such marker. Our objective was investigate kinetics endocan its relationship with inflammation-induced during experimental human endotoxemia. Endothelial function assessed 17 healthy male volunteers before 4 h after administration 2 ng/kg lipopolysaccharide...
Hypoxia and inflammation are closely intertwined phenomena. Critically ill patients often suffer from systemic inflammatory conditions concurrently experience short-lived hypoxia. We evaluated the effects of short-term hypoxia on inflammation, show that it potently attenuates pro-inflammatory cytokine responses during murine endotoxemia. These independent hypoxia-inducible factors (HIFs), but involve augmented adenosine levels, in turn resulting an 2B receptor-mediated post-transcriptional...
Reduced erythrocyte survival and deformability may contribute to the so-called anemia of inflammation observed in septic patients. Erythrocyte structure function are affected by both membrane lipid composition organization. We therefore aimed determine whether these parameters during systemic inflammation.A sensitive matrix-assisted laser desorption ionization time-of-flight mass spectrometric method was used investigate effect plasma components 10 patients with shock healthy volunteers...
We read with great interest the report by Kolb et al. on remimazolam sedation for awake tracheal intubation [1]. In our experience, is a promising sedative agent such cases and we use it as routine when appropriate. The patient described weighed 76 kg, 5 mg bolus of was administered at start procedure, followed three boluses 2.5 2 min intervals (minutes 3, 7). total dose 12.5 which in keeping 15 (administered boluses) an earlier [2]. After initial experience only, changed practice to loading...
Increasing evidence shows that postoperative innate immune dysregulation is associated with delayed recovery and infectious complications. The aim of this study was to compare the effects general versus spinal anesthesia on function during after total hip arthroplasty (THA). This comparative matched cohort used data from two single-center randomized-controlled trials. Patients control group HIPPO received were patients MAGIC who in a 2:1 ratio (general(n = 18); spinal(n 9)). Immune assessed...
Introduction: Difficult airway management is a critical challenge in anesthesia, often necessitating advanced techniques to ensure patient safety. A presented with malignant lesion on the epiglottis, significantly altering anatomy. Flexible rhinolaryngoscopy revealed laryngeal carcinoma affecting entire causing thickening and displacement, which suggested potential for difficult intubation. Methods: Given expected feasibility of bag-mask ventilation front-of-neck access, an asleep intubation...
Abstract Introduction Anemia is a frequently encountered problem during inflammation. Hepcidin an interleukin-6 (IL-6)-induced key modulator of inflammation-associated anemia. Human sepsis prototypical inflammatory syndrome, often complicated by the development However, association between inflammation, hepcidin release and anemia has not been demonstrated in this group patients. Therefore, we explored sepsis-associated Methods 92 consecutive patients were enrolled after presentation on...
Clinical experience suggests that the administration of fluids in human endotoxemia reduces symptoms. In present study, effects a standardised fluid protocol on symptoms, inflammatory and hemodynamic parameters are determined. With approval local ethics committee, 16 healthy volunteers received 2 ng/kg Escherichia coli endotoxin (O:113). After an overnight fast, nine subjects 1.5 l 2.5% glucose/0.45% NaCl hour prior to 150 ml/h during course experiment ('prehydrated group'). Seven only...
Malignant hyperthermia and exertional rhabdomyolysis have conventionally been considered episodic phenotypes that occur in otherwise healthy individuals response to an external trigger. However, recent studies demonstrated a clinical histopathological continuum between patients with history of malignant susceptibility and/or RYR1-related congenital myopathies. We hypothesize or do permanent neuromuscular symptoms episodes. performed prospective cross-sectional observational study features (n...
Clinical experience suggests that the administration of fluids in human endotoxemia reduces symptoms. In present study, effects a standardised fluid protocol on symptoms, inflammatory and hemodynamic parameters are determined. With approval local ethics committee, 16 healthy volunteers received 2 ng/kg Escherichia coli endotoxin (O:113). After an overnight fast, nine subjects 1.5 l 2.5% glucose/0.45% NaCl hour prior to 150 ml/h during course experiment (`prehydrated group'). Seven only...
In this double-blind randomized placebo-controlled trial involving 30 healthy male volunteers we investigated the acute effects of iron loading (single dose 1.25 mg/kg sucrose) and chelation therapy deferasirox) on parameters, oxidative stress, innate immune response, subclinical organ injury during experimental human endotoxemia. The administration sucrose induced a profound increase in plasma malondialdehyde 1 h after (433±37% baseline; P
Introduction Sepsis is the most prevalent cause of Acute Kidney Injury (AKI). Conversely, in some septic patients glomerular filtration rate (GFR) augmented. The role inflammatory response and blood pressure to induce this increased GFR unknown. Herein, relate mediators ‘true’ measured using iohexol clearance kidney injury markers during systemic inflammation healthy volunteers. Methods Twelve subjects underwent experimental endotoxemia (i.v. administration 2 ng/kg Escherichia coli-derived...
There is an ongoing quest for ideal uniform anesthesia regimen that adequately covers all nociceptive stimuli preventing hypertension and tachycardia while minimizing hypotension the need antihypotensive drugs. Recently, ultra-short-acting benzodiazepine remimazolam was approved induction maintenance of general anesthesia. Combining with sevoflurane propofol may combine antiemetic properties propofol, depressing (immobilizing) effect on spinal motor neurons sevoflurane, hemodynamic stability...
To gain insight in the pathogenesis of increased vascular permeability during sepsis, we studied effect plasma obtained human experimental endotoxemia on cultured endothelial monolayers. Eight healthy subjects received an i.v. dose 2 ng/kg Escherichia coli O:113 lipopolysaccharide (LPS). The concentration various mediators that supposedly induce was measured over time. Plasmas were before, and 4 h after administration LPS added to umbilical venular cells semipermeable membranes.The...
A higher body mass index (BMI) appears to be associated with lower mortality in critically ill patients, possibly explained by an altered innate immune response. However, the precise relationship between BMI and response humans vivo is unknown. We investigated systemic cytokine during experimental human endotoxemia. Endotoxemia was induced 112 healthy male volunteers intravenous administration of 2 ng/kg Escherichia coli endotoxin. Plasma concentrations TNF-α, IL-6, IL-10 IL-1RA were...
To determine the safety and efficacy of human chorionic gonadotropin hormone-derivative EA-230 in cardiac surgery patients. Cardiac induces systemic inflammation may impair renal function, affecting patient outcome. exerted immunomodulatory renoprotective effects preclinical models was safe showed phase I II studies. Double-blinded, placebo-controlled, randomized study. Collaboration Cardiothoracic Surgery, Anesthesiology, Intensive Care departments a tertiary hospital Netherlands. One...
Background: Variants in RYR1, the gene encoding ryanodine receptor-1, can give rise to a wide spectrum of neuromuscular conditions. Muscle imaging abnormalities have been demonstrated isolated cases patients with history RYR1-related malignant hyperthermia (MH) susceptibility. Objective: To provide insights into type and prevalence muscle ultrasound hypertrophy carrying gain-of-function RYR1 variants associated MH susceptibility contribute delineating wider phenotype, optimizing diagnostic...