A5070031114- Animal Virus Infections Studies
- Virus-based gene therapy research
- Genetic and phenotypic traits in livestock
- Cancer-related molecular mechanisms research
- Genetic Mapping and Diversity in Plants and Animals
- Animal Disease Management and Epidemiology
- RNA modifications and cancer
- Parvovirus B19 Infection Studies
- Virology and Viral Diseases
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- RNA Research and Splicing
- Viral gastroenteritis research and epidemiology
- Herpesvirus Infections and Treatments
- Digestive system and related health
- Bacteriophages and microbial interactions
- Vector-Borne Animal Diseases
- Mosquito-borne diseases and control
- Animal Genetics and Reproduction
- Genomic variations and chromosomal abnormalities
- Malaria Research and Control
- Adipose Tissue and Metabolism
- vaccines and immunoinformatics approaches
- Mobile Agent-Based Network Management
- Genetic Syndromes and Imprinting
Chinese Academy of Agricultural Sciences
2010-2024
Institute of Animal Sciences
2016-2024
Ministry of Agriculture and Rural Affairs
2023
Ministère de l'Agriculture et de la Souveraineté alimentaire
2021
Ministry of Agriculture & Farmers Welfare
2021
Icahn School of Medicine at Mount Sinai
2013-2016
International Neuromodulation Society
2013
Academy of Military Medical Sciences
2009-2011
Chinese Academy of Medical Sciences & Peking Union Medical College
2009-2011
National Center of Biomedical Analysis
2010
Ad hoc networks are a new wireless networking paradigm for mobile hosts. In this paper, we designed an intelligent transport system. The ITS (intelligent system) includes two big function modules: Information processing application system and Road condition information transferring main task of the road module is in charge exchange car inside, to road. works ad network, call network VANET (vehicular ad-hoc network) . Vehicular likely become most relevant form networks. For sake insuring can...
Abstract Background The development of skeletal muscle in pigs during the embryonic stage is precisely regulated by transcriptional mechanisms, which depend on chromatin accessibility. However, how accessibility plays a regulatory role has not been reported. To gain insight into landscape and associated genome-wide transcriptome development, we performed ATAC-seq RNA-seq analyses from pig embryos at 45, 70 100 days post coitus (dpc). Results In total, 21,638, 35,447 60,181 unique regions (or...
The Ashkenazi Jewish ( AJ ) population has an increased risk for a variety of recessive diseases due to historical founder effects and genetic drift. For some, the disease‐causing mutations have been identified well‐characterized, but others, further study is necessary. purpose this assess carrier frequencies 85 pathogenic variants causative 29 conditions in population. Up 3000 individuals were genotyped by Luminex MagPlex ® ‐ TAG ™ bead array or Agena Bioscience MassARRAY assays. We seven...
Gestation length (GL) plays an important role in piglet maturation of major organs and development body, while the genetic molecular markers GL have not been extensively identified. In this study, according to 5,662 effective records 3,072 sows, heritability repeatability were estimated through dmuai DMU Version 6.5.1 with a model, namely, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="m1"><mml:mrow><mml:msup><mml:mi>h</mml:mi><mml:mn>2</mml:mn></mml:msup></mml:mrow></mml:math>...
Hoyeraal–Hreidarsson syndrome ( HH ) is a clinically severe variant of dyskeratosis congenita DC ), characterized by cerebellar hypoplasia, microcephaly, intrauterine growth retardation, and immunodeficiency in addition to features . Germline mutations the RTEL1 gene have recently been identified as causative In this study, carrier frequency for five that occurred individuals Ashkenazi Jewish descent was investigated order advise on including them existing clinical mutation panels...
The number of vertebrae (NV), especially the thoracic (NTV), varies among pig breeds. NTV is controlled by vertebral segmentation and somites during embryonic development. Although there a high correlation between NV, studies on fixed NV have mainly considered absolute numbers instead segmentation. Therefore, this study aimed to discover variants associated with considering effect in pigs. 542 F2 individuals from Large White × Minzhu crossbreed were recorded. All animals genotyped for VRTN...
Increasing intramuscular fat (IMF) content can enhance the sensory quality of meat, including tenderness, juiciness, flavor, and color. Genome-wide association study RNA-sequencing (RNA-seq) analysis were used to identify candidate IMF genes in Beijing Black pigs, a popular species among consumers northern China. Two three single nucleotide polymorphisms significantly associated with SSC13 SSC15 respectively. Solute carrier family 4 member 7 (SLC4A7) on insulin induced gene 2 (INSIG2),...
To study the characteristics of genes and metabolites related to intramuscular fat (IMF) content with less influence by breed background individual differences, skeletal muscle samples from 40 Beijing black pigs either high or low IMF were used perform transcriptome metabolome analyses. About 99 (twofold-change) differentially expressed. Up-regulated in mainly metabolism. The key charge deposition are ADIPOQ, CIDEC, CYP4B1, DGAT2, LEP, OPRL1, PLIN1, SCD, THRSP. KLHL40, TRAFD1, HSPA6 novel...
As one of the few animals with variation in number rib pairs (RIB), pig is a good model to study mechanism RIB regulation. Quantitative trait loci (QTL) for porcine are present on Sus scrofa chromosome 7 (SSC7). Although several candidate genes exist this QTL region SSC7, causal gene has yet be verified. Beijing Black 14-17 population mining and 1104 individuals were genotyped using Illumina Porcine 50K BeadChip. A total 14 SNPs from 95.49 97.78 Mb SSC7 showed genome-wide significant...
The study of the association between polymorphic Alu insertions [human leukocyte antigen (HLA)-Alu] at five loci and HLA class I alleles two allows for better identification origins evolution region haplotypes in different populations. In present study, we determined frequencies HLA-Alus their associations with HLA-A -B Han, Wa, Maonan, Jinuo Our results showed a strong AluHG insertion HLA-A*02 all populations studied; however, AluHJ HLA-A*1101 HLA-A*2402 AluHF HLA-A*2601 were only observed...
Abstract Encephalomyocarditis virus (EMCV) is as a potential zoonotic agent with wide host range. Here, we describe an EMC isolate, identified EMCV C15, which was successfully obtained from the serum of dogs animal hospitals. Virus production in cell culture confirmed by EMCV-specific real-time RT-PCR, indirect immunofluorescence assays and electron microscopy. In addition, open reading frame sequence (ORF) C15 determined. From comparison phylogenetic analysis among 24 reference strains, it...
A gold nanoparticle (GNP) probe-based assay (GNPA) modified from the bio-barcode (BCA) was developed for ultrasensitive detection of ricin, a potential biothreat agent. In GNPA, chain ricin captured by GNP probe coated with polyclonal antibodies and single-stranded signal DNA. magnetic microparticle (MMP) monoclonal antibody then added to form an immuno-complex. After being magnetically separated, immuno-complex containing DNA characterized PCR real-time PCR. limit 10−2 fg/ml determined...
Protoparvoviruses, widespread among cats and wild animals, are responsible for leukopenia. Feline panleukopenia virus (FPLV) in domestic is genetically diverse some strains may differ from those used vaccination. The presence of FPLV two Hebei Province China was identified by polymerase chain reaction. Samples these animals were to isolate CRFK cells genome sequencing. Phylogenetic analysis performed compare our isolates with available sequences FPLV, mink parvovirus (MEV) canine (CPV)....
RNA-Seq technology is widely used to analyze global changes in the transcriptome and investigate influence on relevant phenotypic traits. Beijing Black pigs show differences growth rate meat quality compared western pig breeds. However, molecular mechanisms responsible for such remain unknown. In this study, longissimus dorsi muscles from Yorkshire were construct RNA libraries perform RNA-seq. Significantly different expressions observed 1051 mRNAs, 322 lncRNAs, 82 circRNAs. GO KEGG pathway...
Porcine epidemic diarrhea virus (PEDV) causes porcine (PED), a highly infectious disease, which has resulted in huge economic losses for the pig industry. To date, pathogenic and immune response mechanism was not particularly clear. The purpose of this study to investigate responses pigs infected with PEDV.In study, 12 Min were randomly selected without taking colostrum. At 3 days old, eight piglets 1 mL PEDV solution (10 TCID50/ml), remaining four handled by 0.9% normal saline. Within age 7...
RNA editing is an essential process for modifying nucleotides at specific sites during post-transcription in many species. However, its genomic landscape and characters have not been systematically explored the bovine genome. In present study, we characterized global profiles from 50 samples of cattle revealed a range different tissues. Most were significantly enriched BovB-derived SINEs, especially dispersed Bov-tAs, which likely forms dsRNA structures similar to primate-specific Alu...
Abstract A rapid real-time polymerase chain reaction (RT-PCR) for detecting West Nile virus (WNV) was established. Primers were designed according to the sequence of capsid protein gene WNV by Primer Premier 5.0. In this way, an inexpensive assay using intercalating dye SYBR Green I developed and validated. The amplifying curve showed that method could successfully amplify 10 2 copies/μl gene, while reference Japanese encephalitis (JEV) blank control all negative. Tenfold successive...