A5101914291- Parvovirus B19 Infection Studies
- Virus-based gene therapy research
- Xenotransplantation and immune response
- Viral gastroenteritis research and epidemiology
- Dermatological and COVID-19 studies
- Animal Genetics and Reproduction
- Protease and Inhibitor Mechanisms
- Protein purification and stability
- CRISPR and Genetic Engineering
- Peptidase Inhibition and Analysis
- Iron Metabolism and Disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Blood transfusion and management
- Cytomegalovirus and herpesvirus research
- Plant and Fungal Species Descriptions
- Blood properties and coagulation
- Biochemical and Molecular Research
- Prenatal Screening and Diagnostics
- Mesenchymal stem cell research
- T-cell and B-cell Immunology
- Reproductive tract infections research
- Syphilis Diagnosis and Treatment
- Pesticide Exposure and Toxicity
- Surface Modification and Superhydrophobicity
- Molecular Biology Techniques and Applications
Wuxi People's Hospital
2024
Xian Yang Central Hospital
2024
Academy of Military Medical Sciences
2008-2023
National Medical Products Administration
2022
Hangzhou Medical College
2022
Zhejiang Provincial People's Hospital
2022
Guangzhou Blood Center
2016-2017
National Center of Biomedical Analysis
2010-2016
Soochow University
2011
Background A recent study provided evidence that farmed rabbits in China harbor a novel hepatitis E virus (HEV) genotype. Although the rabbit HEV isolate had 77–79% nucleotide identity to mammalian genotypes 1 4, their genomic organization is very similar. Since are used widely experimentally, including as models of infection, we investigated whether they constitute an appropriate animal model for human infection. Methods Forty-two SPF were divided randomly into eleven groups and inoculated...
Human parvovirus B19 (B19V) is a frequent contaminant of blood and plasma-derived medicinal products. To ensure the quality safety products, European regulations, Plasma Protein Therapeutics Association (PPTA) standard FDA guidelines require testing manufacturing plasma for DNA to limit load this virus. In China, however, there have been no related documentation technical guiding principles monitoring B19V, moreover, an adequate level information on prevalence B19V in Chinese donations not...
Human parvovirus B19 (B19V) is a frequent contaminant of blood and plasma-derived medicinal products. Three distinct genotypes B19V have been identified. The distribution the three has investigated in various regions or countries. However, China, data on existence different are limited. One hundred eighteen B19V-DNA positive source plasma pool samples collected from Chinese products manufacturers were analyzed. subgenomic NS1/VP1u region junction was amplified by nested PCR. These then...
BACKGROUND The pathogenesis of transfusion‐related acute lung injury (TRALI) progress is incompletely understood, and specific therapies for TRALI are lacking. Alveolar macrophages (AMs) critical initiation resolution inflammation. However, the role AMs in TRALI‐associated failure poorly understood. STUDY DESIGN AND METHODS Mouse model vivo imaging interleukin (IL)‐6 activation was established by intratracheal instillation a lentiviral vector carrying luciferase reporter gene. mouse produced...
Abstract: Human parvovirus B19 (B19V) is a common human pathogen which associated with broad spectrum of clinical manifestations. Since B19V can be transmitted via blood and plasma derivatives, it has been considered to pose great risk in transfusion safety. the early 21st century, European Pharmacopoeia, Plasma Protein Therapeutics Association U.S. Food Drug Administration have proposed list guidelines reduce transmission by derivatives. In terms blood, some countries implement management...
Context α2-Macroglobulin (α2-M) is believed to be a potential anti-irradiation agent, but related mechanisms remains unclear.Objective We investigated the irradiation protective effect of α2-M.Materials and methods A total 10 Gy dose was used damage human skin fibroblasts. The influence α2-M (100 µg/mL) on proliferation, migration, invasion apoptosis fibroblasts observed using Cell Counting Kit-8 (CCK8), wound healing, transwell, flow cytometry. Malondialdehyde, superoxide dismutase catalase...
A gold nanoparticle (GNP) probe-based assay (GNPA) modified from the bio-barcode (BCA) was developed for ultrasensitive detection of ricin, a potential biothreat agent. In GNPA, chain ricin captured by GNP probe coated with polyclonal antibodies and single-stranded signal DNA. magnetic microparticle (MMP) monoclonal antibody then added to form an immuno-complex. After being magnetically separated, immuno-complex containing DNA characterized PCR real-time PCR. limit 10−2 fg/ml determined...
Cryopreserved platelets (cPLTs) can be stored for years and are mainly used in military settings. However, the commonly cryoprotectant dimethyl sulphoxide (DMSO) has toxic side effects when utilized high quantities. We developed a novel method to aseptically remove DMSO from thawed cPLTs by dialysis.One unit of (N = 6) was mixed with 75 mL 27% within 4 days after collection at -80°C 1 week. The platelet counts, distribution width, mean volume (MPV), activity, release, aggregation, metabolism...
Abstract α1‐Antitrypsin (AAT) is widely used to treat patients with congenital AAT deficiency. Cohn Fraction IV (Cohn F IV) normally discarded during the manufacturing process of albumin but contains approximately 33% plasma AAT. We established a new for large‐scale purification from it. liquid chromatography‐electrospray ionization‐tandem mass spectrometry and high‐performance chromatography were applied qualitative identification composition analysis, respectively. Stabilizers optimized...
Human parvovirus B19 (B19V) and human 4 (PARV4) are known to infect humans transmit through contaminated blood products. Globally, three genotypes of B19V, as well PARV4, have been identified, respectively. The existence different B19V in Chinese plasma donors has investigated, however, the data regarding PARV4 were not available. main objective this study is identify circulating donors. By using a duplex quantitative polymerase chain reaction assay adapted for all 78 source pools...
BACKGROUND α2‐Macroglobulin (α2‐M) has a curative effect on radiation injury. Virus transmission through plasma derivatives is still not risk‐free. Effect of dry heat α2‐M activity and virus inactivation by in new manufacturing process were studied. STUDY DESIGN AND METHODS Effects 100°C for 30 minutes, 80°C 72 hours, lyophilization detected, stabilizing agents optimized. treatment at minutes been tested range viruses characteristics change was investigated. RESULTS More than 90 80% recovery...
Mesenchymal stem/stromal cells (MSCs) are adult stem that were originally isolated from bone marrow. In contrast to long bone-derived MSCs have been extensively characterized, our knowledge regarding flat bones (e.g., cranial bones) remain less clear. this study, purified human marrow (CB-MSCs) and their transdifferentiation capacity immunomodulatory functions further characterized. Phenotypic analysis of CB-MSCs demonstrated high expression CD73, CD90, CD105 while negative for CD14, CD34,...
Human platelets (PLTs) are vulnerable to unfavorable conditions, and their adequate supply is limited by strict transportation conditions. We report here that PLTs preserved under three-dimensional (3D) conditions using novel biomimetic nanofiber peptides showed reduced apoptosis compared with classical stored at 22 °C facilitated the storage of PLTs. The mechanism PLT 3D preservation involves formation cross-links a nanofibrous network self-assembled peptide scaffold material physiological...
Pig to human xenotransplantation is considered be a possible approach alleviate the shortage of allografts. Porcine endogenous retrovirus (PERV) most significant pathogen in xenotransplantation. We screened for pigs that consistently did not transmit human-tropic replication competent PERVs (HTRC PERVs), namely, non-transmitting pigs. Then, we conducted whole-genome resequencing and full-length transcriptome sequencing further investigate sequence characteristics one pig. Using vitro...
Encouraging advances in both regenerative medicine and tissue engineering with stem cells require a short-term preservation protocol to provide enough time for quality control or the transportation of cell products from manufacturing facilities clinical destinations. The hypothermic under refrigerated conditions (2-8 °C) their specific culture medium provides an alternative low-cost method cryopreservation commercial fluid storage. However, most are vulnerable hypothermia, which might result...
Analyses of three B19V 1a/3b recombinant sequences. The left part each panel was the results bootscan analysis. At right were neighbour-joining trees established on basis fragments between breakpoints, as indicated by a plot sequence. (JPG 4401Â kb)