A5100611355- Animal Virus Infections Studies
- Virus-based gene therapy research
- Parvovirus B19 Infection Studies
- Hepatitis Viruses Studies and Epidemiology
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Vector-Borne Animal Diseases
- Dermatological and COVID-19 studies
- Advanced biosensing and bioanalysis techniques
- Peptidase Inhibition and Analysis
- Liver Disease Diagnosis and Treatment
- Protein purification and stability
- Xenotransplantation and immune response
- Hepatitis B Virus Studies
- Influenza Virus Research Studies
- Hepatitis C virus research
- Animal Genetics and Reproduction
- Protease and Inhibitor Mechanisms
- Cytomegalovirus and herpesvirus research
- Metal-Organic Frameworks: Synthesis and Applications
- Iron Metabolism and Disorders
- Blood properties and coagulation
- Wireless Power Transfer Systems
- Viral Infectious Diseases and Gene Expression in Insects
Qingdao Agricultural University
2025
Chinese Academy of Fishery Sciences
2024
Shanghai Ocean University
2024
Dalian Polytechnic University
2022-2023
Academy of Military Medical Sciences
2004-2022
Air Force Medical University
2021
Wuhan University of Technology
2020
Jiamusi University
2020
Guangzhou Blood Center
2007-2017
National Center of Biomedical Analysis
2008-2016
The lack of efficient antigen and activator delivery systems, as well the restricted migration dendritic cells (DCs) to secondary lymph organs, dramatically limits DC-based adoptive immunotherapy. We selected two spherical gold nanoparticle (AuNP)-based vehicles optimal size for delivery. Their combination (termed NanoAu-Cocktail) was associated with dual targeting CpG oligonucleotides (CpG-ODNs) an OVA peptide (OVAp) DC subcellular compartments, inducing enhanced cross-presentation,...
Background A recent study provided evidence that farmed rabbits in China harbor a novel hepatitis E virus (HEV) genotype. Although the rabbit HEV isolate had 77–79% nucleotide identity to mammalian genotypes 1 4, their genomic organization is very similar. Since are used widely experimentally, including as models of infection, we investigated whether they constitute an appropriate animal model for human infection. Methods Forty-two SPF were divided randomly into eleven groups and inoculated...
Precise identification and analysis of multiple protein biomarkers on the surface breast cancer cell-derived extracellular vesicles (BC-EVs) are great significance for noninvasive diagnosis subtypes, but it remains a major challenge owing to their high heterogeneity low abundance. Herein, we established CRISPR-based homogeneous electrochemical strategy near-zero background ultrasensitive detection BC-EVs. To realize high-performance capture isolation BC-EVs, fluidity-enhanced magnetic...
The development of vaccination and novel therapy for hepatitis C virus (HCV) has been hampered by the lack suitable small-animal models. GB B (GBV-B), closely related to HCV, causes viral in common marmosets (Callithrix jacchue jacchus) might represent an attractive surrogate model HCV infection. However, differences exist between GBV-B spite a short genetic distance two viruses. Here we report infected with HCV/GBV-B chimeras containing structural genes coding either whole core envelope...
Human parvovirus B19 (B19V) is a frequent contaminant of blood and plasma-derived medicinal products. To ensure the quality safety products, European regulations, Plasma Protein Therapeutics Association (PPTA) standard FDA guidelines require testing manufacturing plasma for DNA to limit load this virus. In China, however, there have been no related documentation technical guiding principles monitoring B19V, moreover, an adequate level information on prevalence B19V in Chinese donations not...
Human parvovirus B19 (B19V) is a frequent contaminant of blood and plasma-derived medicinal products. Three distinct genotypes B19V have been identified. The distribution the three has investigated in various regions or countries. However, China, data on existence different are limited. One hundred eighteen B19V-DNA positive source plasma pool samples collected from Chinese products manufacturers were analyzed. subgenomic NS1/VP1u region junction was amplified by nested PCR. These then...
Abstract Nine hundred and four serum samples were collected from pigs 16 pig farms in China's Hunan province tested for anti‐hepatitis E virus (HEV) antibody the HEV capsid antigen using EIAs. Of 904 samples, 617 (68.3%) 57 (6.3%) positive anti‐HEV antigen, respectively. The prevalence of antibodies detection varied significantly among different ( P < 0.01). positivity rate occurrence high titer higher above, than those below, 3 months age, whilst did not differ between two groups. Based...
Hemorrhagic fever with renal syndrome (HFRS) is a regional infectious disease of epidemic potential caused by the Hantaan virus (HTNV). Red blood cells (RBCs) are major components peripheral blood. However, pathological changes in RBCs and underlying mechanisms during HTNV infection remain largely unclear. Therefore, this study sought to explore HFRS patients. We isolated PBMCs from patients performed single-cell RNA sequencing. The results showed that clusters could be classified as...
Context α2-Macroglobulin (α2-M) is believed to be a potential anti-irradiation agent, but related mechanisms remains unclear.Objective We investigated the irradiation protective effect of α2-M.Materials and methods A total 10 Gy dose was used damage human skin fibroblasts. The influence α2-M (100 µg/mL) on proliferation, migration, invasion apoptosis fibroblasts observed using Cell Counting Kit-8 (CCK8), wound healing, transwell, flow cytometry. Malondialdehyde, superoxide dismutase catalase...
In this study, we investigated the body characteristics, carotenoid composition, and nutritional quality of Eriocheir sinensis with different hepatopancreas redness (a*). We distributed crabs into two groups based on a* values compared their chroma, protein, lipid, total sugar, amino acid, fatty acid content via paired t-test. The results revealed that relationships between crab are sex specific. female crabs, differences in characteristics were evident mainly hepatopancreases ovaries....
Complete ligation of the common carotid artery near its bifurcation induces neointimal formation due to smooth muscle cell proliferation in normolipidemic wild-type mice, but it was unknown what would happen hyperlipidemic apolipoprotein E-deficient (Apoe-/-) mice. Examination these mice revealed rapid development atherosclerotic lesions completely ligated arteries within 4 weeks. Mice were fed a Western diet, starting 1 week before ligation, or chow diet. Foam formed as early after diet and...
Background A flow‐based treatment device using riboflavin and ultraviolet ( UV ) light was developed to inactivate viruses in fresh‐frozen plasma FFP ). The objective of this study evaluate the vitro effectiveness virus inactivation changes protein quality treated with device. Study Design Methods ‐contaminating were a one‐pass linear flow infectivity measured established biologic assays. Real‐time polymerase chain reaction PCR performed detect damage viral nucleotides after treatment....
Xenotransplantation from animals has been considered to be a preferable approach alleviate the shortage of human allografts. Pigs are most suitable candidate because anatomical and physiological similarities shared with humans as well ethical concerns. However, it may associated risk transmission infectious porcine pathogens. Porcine endogenous retroviruses (PERVs) particular concern they have shown infect cells in vitro. To date, researches on molecular characteristics potential...
Abstract α1‐Antitrypsin (AAT) is widely used to treat patients with congenital AAT deficiency. Cohn Fraction IV (Cohn F IV) normally discarded during the manufacturing process of albumin but contains approximately 33% plasma AAT. We established a new for large‐scale purification from it. liquid chromatography‐electrospray ionization‐tandem mass spectrometry and high‐performance chromatography were applied qualitative identification composition analysis, respectively. Stabilizers optimized...
Human parvovirus B19 (B19V) and human 4 (PARV4) are known to infect humans transmit through contaminated blood products. Globally, three genotypes of B19V, as well PARV4, have been identified, respectively. The existence different B19V in Chinese plasma donors has investigated, however, the data regarding PARV4 were not available. main objective this study is identify circulating donors. By using a duplex quantitative polymerase chain reaction assay adapted for all 78 source pools...
BACKGROUND α2‐Macroglobulin (α2‐M) has a curative effect on radiation injury. Virus transmission through plasma derivatives is still not risk‐free. Effect of dry heat α2‐M activity and virus inactivation by in new manufacturing process were studied. STUDY DESIGN AND METHODS Effects 100°C for 30 minutes, 80°C 72 hours, lyophilization detected, stabilizing agents optimized. treatment at minutes been tested range viruses characteristics change was investigated. RESULTS More than 90 80% recovery...
Radiotherapy is an effective treatment modality in the clinical of breast cancer. The present work investigated effect 3,3'-diindolylmethane (DIM) on γ-irradiation sensitizing human carcinoma.Cell survival, intracellular ROS levels, cell cycle distribution, apoptosis, and expression proteins related to apoptosis were measured with MTT assays, flow cytometry, Western blot analysis, respectively.In vitro DIM plus arrested activity G2/M phase cycle, increased level, significantly suppressed...