A5103200114- Antifungal resistance and susceptibility
- Oral microbiology and periodontitis research
- Bacterial biofilms and quorum sensing
- Fungal Infections and Studies
- Peptidase Inhibition and Analysis
- Antimicrobial Resistance in Staphylococcus
- Dental materials and restorations
- Dental Health and Care Utilization
- Infective Endocarditis Diagnosis and Management
- Microbial Metabolism and Applications
- Endodontics and Root Canal Treatments
- Bacterial Infections and Vaccines
- Bacterial Identification and Susceptibility Testing
- Probiotics and Fermented Foods
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Bee Products Chemical Analysis
- Mycotoxins in Agriculture and Food
- Advanced biosensing and bioanalysis techniques
- Plant and animal studies
- Pneumocystis jirovecii pneumonia detection and treatment
University of Technology Sydney
2021
National University of Singapore
2012-2020
Candida albicans, a major opportunistic fungal pathogen, is frequently found together with Streptococcus mutans in dental biofilms associated severe childhood caries (tooth decay), prevalent pediatric oral disease. However, the impact of this cross-kingdom relationship on C. albicans remains largely uncharacterized. Here, we employed novel quantitative proteomics approach conjunction transcriptomic profiling to unravel molecular pathways when cocultured S. mixed biofilms. RNA sequencing and...
Enterococcus faecalis is a bacterial pathogen associated with both endodontic and systemic infections. The biofilm formation ability of E. plays key role in its virulence drug resistance attributes. biofilms on implanted medical devices often results treatment failure. In the present study, we report protein markers using iTRAQ-based quantitative proteomics approach. order to elucidate biofilm-associated markers, investigated proteome strong weak biofilm-forming clinical isolates comparison...
Candida albicans is a major fungal pathogen causing lethal infections in immunocompromised patients. C. forms antifungal tolerant biofilms contributing significantly to therapeutic failure. The recently established haploid biofilm model provides new toolbox uncover the mechanism governing higher tolerance of biofilms. Here, we comprehensively examined proteomics and susceptibility standard diploid (SC5314 BWP17) stable (GZY792 GZY803) strains Subsequent downstream analyses identified alkyl...
Abstract Clinical isolates of the fungal human pathogen Candida albicans are invariably diploid and heterozygous, impeding genetic study. Recent isolation C. haploids opens opportunities to apply technologies unfeasible in diploids. However, doubts remain on whether haploids, derived from chromosome loss, can represent Here, we use investigate biofilm, a key virulence attribute. We conducted first comprehensive characterization biofilm formation comparison with demonstrate that form biofilms...
Honey adulteration is a problem that effects the global honey industry and specifically, has been discovered in Australian market. Common methods of include dilution with sugar syrup substitutes mislabelling floral geographic origin(s) honey. Current authentication tools rely on molecular variability between different honeys, identifying unique chemical profiles and/or DNA signatures characteristic particular known to contain plant miRNAs derived from its source. To explore composition RNA...
Antifungal agents for the treatment of Candida albicans infections are limited. We recently discovered a novel antifungal small molecule, SM21, with promising in vivo activity. Herein, we employed newly developed C. haploid toolbox to uncover mechanism action SM21. Comprehensive RNASeq analyses on susceptible GZY803 strain revealed significant gene expression changes related mitochondria when exposed Mitochondrial structure visualization, ATP generation, reactive oxygen species (ROS),...
Abstract Candida albicans is a major fungal pathogen, accounting for approximately 15% of healthcare infections with associated mortality as high 40% in the case systemic candidiasis. Antifungal agents C. are limited, and rising resistance an inevitable problem. Therefore, understanding mechanism behind antifungal responses among top research focuses combating infections. Herein, recently developed haploid model employed to examine association between mitochondrial fission, regulated by...
Candida albicans is a commensal polymorphic and opportunistic fungus, which usually resides as small community in the oral cavities of majority humans. The latter eco-system presents this yeast varied opportunities for mutualistic interactions with other cohabitant bacteria, that synergizes its persistence pathogenicity. Collectively, these communities live within complex plaque biofilms may adversely affect health increase proclivity candidiasis. proteome such myriad interkingdom are...