A5089769563- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Animal Genetics and Reproduction
- Insect behavior and control techniques
- Insect Pest Control Strategies
- Viral Infectious Diseases and Gene Expression in Insects
- Insect and Pesticide Research
- Bone health and osteoporosis research
- Bone Tissue Engineering Materials
- RNA Interference and Gene Delivery
- Bone health and treatments
- Insect and Arachnid Ecology and Behavior
Justus-Liebig-Universität Gießen
2013-2024
The first objective was to investigate new bone formation in a critical-size metaphyseal defect the femur of ovariectomized rats filled with strontium modified calcium phosphate cement (SrCPC) compared (CPC) and empty defects. Second, detection release from materials as well collagen mass distribution fracture should be targeted by time flight secondary ion spectrometry (TOF-SIMS). 45 female Sprague-Dawley were randomly assigned three different treatment groups: (1) SrCPC (n = 15), (2) CPC...
In this study, we report the complexities and challenges associated with achieving robust RNA interference (RNAi)-mediated gene knockdown in mosquitoes Aedes aegypti albopictus, a pivotal approach for genetic analysis vector control. Despite RNAi’s potential species-specific targeting, our independent efforts to establish oral delivery of RNAi identifying genes critical mosquito development fitness encountered significant challenges, failing reproduce previously reported potent effects. We...
Creating transgenic insects is a key technology in insect genetics and molecular biology. A widely used instrument transgenesis the piggyBac transposase, resulting essentially random genomic integrations. In contrast, site-specific recombinases allow targeted integration of transgene construct into specific target site. Both strategies, however, often face limitations due to low efficiencies. We aimed enhance efficiencies by utilizing capped mRNA as source transposase or recombinase instead...
Genetic control strategies such as the Release of Insects Carrying a Dominant Lethal (RIDL) gene and Transgenic Embryonic Sexing System (TESS) have been demonstrated in laboratory and/or deployed field. These are based on tetracycline-off (Tet-off) systems which regulated by antibiotics Tet doxycycline (Dox). Here, we generated several Tet-off constructs carrying reporter cassette mediated 2A peptide. Different concentrations (0.1, 10, 100, 500, 1,000 μg/mL) types (Tet or Dox) were used to...
Creating transgenic insects is a key technology in insect genetics and molecular biology. A widely used instrument transgenesis the piggyBac transposase, resulting essentially ran-dom genomic integrations. In contrast, site-specific recombinases allow targeted integration of transgene construct into specific target site. Both strategies, however, often face limitations due to low rates. We aimed enhance rates by utilizing capped mRNA as source transposase or recombinase instead helper...
Climate change and global movements of people goods have accelerated the spread invasive species, including insects that vector infectious diseases, which threaten health more than half world's population. Increasing research efforts to control these diseases include study - pathogen interactions, involving handling pathogen-infected under biosafety level (BSL) 2 3 conditions. Like microbiology BSL-3 laboratories, insectaries are usually subjected fixed-term or emergency room decontamination...
The transgenic strain of the Mediterranean fruit fly (medfly), Ceratitis capitata (Wied.) VIENNA 8 1260, developed from classical genetic sexing 8, has two molecular markers that exhibit red fluorescence in body and green testicles sperm. These traits offer a precise tool to discriminate between mass-reared sterile males wild fertile males, they could potentially increase effectiveness control programs for this pest. To assess risk horizontal transfer transgenes natural ecosystems, we used...