A5074164788- Plant Stress Responses and Tolerance
- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Plant Micronutrient Interactions and Effects
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Genetic Mapping and Diversity in Plants and Animals
- Plant Physiology and Cultivation Studies
- Plant pathogens and resistance mechanisms
- RNA and protein synthesis mechanisms
- Genetics and Plant Breeding
- Plant Gene Expression Analysis
- Crop Yield and Soil Fertility
- Plant biochemistry and biosynthesis
- Plant-Microbe Interactions and Immunity
- Molecular Biology Techniques and Applications
- Postharvest Quality and Shelf Life Management
- Genomics and Phylogenetic Studies
- Polysaccharides and Plant Cell Walls
U.S. Salinity Laboratory
2016-2024
United States Department of Agriculture
2010-2024
Agricultural Research Service
2010-2024
RELX Group (Netherlands)
2020
University of Wisconsin–Stevens Point
2007-2016
Iowa State University
2002-2012
Joint Genome Institute
2010
Obihiro University of Agriculture and Veterinary Medicine
2009
University of Nebraska–Lincoln
2001-2004
Washington State University
2004
Soybean (Glycine max) is one of the most important crop plants for seed protein and oil content, its capacity to fix atmospheric nitrogen through symbioses with soil-borne microorganisms. We sequenced 1.1-gigabase genome by a whole-genome shotgun approach integrated it physical high-density genetic maps create chromosome-scale draft sequence assembly. predict 46,430 protein-coding genes, 70% more than Arabidopsis similar poplar which, like soybean, an ancient polyploid (palaeopolyploid)....
Abstract Because of the huge size common wheat (Triticum aestivum L., 2n = 6x 42, AABBDD) genome 17,300 Mb, sequencing and mapping expressed portion is a logical first step for gene discovery. Here we report 7104 sequence tag (EST) unigenes by Southern hybridization into chromosome bin map using set aneuploids deletion stocks. Each EST detected mean 4.8 restriction fragments 2.8 loci. More loci were mapped in B (5774) than A (5173) or D (5146) genomes. The density was significantly higher B....
Twelve alfalfa genotypes that were selected for biomass under salinity, differences in Na and Cl concentrations shoots K/Na ratio evaluated this long-term salinity experiment. The plants cloned to reduce genetic variability within each genotype. Salt tolerance (ST) index of the ranged from 0.39 1. most salt-tolerant SISA14-1 (G03) AZ-90ST (G10), top performers biomass, exhibited least effect on shoot number height. accumulated low salinity. Most a net reduction Ca, Mg, P, Fe, Cu, while Mn Zn...
Abstract The short arm of Triticeae homeologous group 1 chromosomes is known to contain many agronomically important genes. objectives this study were physically localize gene-containing regions the arm, enrich these with markers, and distribution genes recombination. We focused on major gene-rich region (“1S0.8 region”) identified 75 useful along 93 RFLP markers by comparing 35 different maps Poaceae species. tested gel blot DNA analysis wheat nullisomic-tetrasomic lines, ditelosomic four...
Abstract Resistance of soybean against the oomycete pathogen Phytophthora sojae is conferred by a series Rps genes. We have characterized disease resistance gene-like sequence NBSRps4/6 that was introgressed into lines along with Rps4 or Rps6. High-resolution genetic mapping established cosegregates Rps4. Two mutants, M1 and M2, showing rearrangements in region were identified from analyses 82 F1's 201 selfed HARO4272 plants containing Fingerprints these mutants are identical to those for...
Systemic acquired resistance (SAR) is induced in non-inoculated leaves following infection with certain pathogenic strains. SAR effective against many pathogens. Salicylic acid (SA) a signaling molecule of the pathway. The development associated induction pathogenesis related (PR) genes. Arabidopsis non-expressor PR1 (NPR1) regulatory gene SA signal pathway 123. soybean was first reported Colletotrichum trancatum that causes anthracnose disease. We investigated if regulated by pathway,...
Abstract The almond crop has high economic importance on a global scale, but its sensitivity to salinity stress can cause severe yield losses. Salt-tolerant rootstocks are vital for feasibility under saline conditions. Two commercial submitted salinity, and evaluated through different parameters, had contrasting results with the survival rates of 90.6% ‘Rootpac 40’ (tolerant) 38.9% ‘Nemaguard’ (sensitive) (Electrical conductivity water = 3 dS m −1 ). Under accumulated less Na Cl more K in...
Abstract The focus of this study was to analyze the content, distribution, and comparative genome relationships 996 chromosome bin-mapped expressed sequence tags (ESTs) accounting for 2266 restriction fragments (loci) on homoeologous group 3 chromosomes hexaploid wheat (Triticum aestivum L.). Of these loci, 634, 884, 748 were mapped 3A, 3B, 3D, respectively. individual bin maps revealed bins with a high density ESTs in distal region low proximal arms, exception 3DS 3DL. These distributions...
Abstract The objectives of this study were to isolate and physically localize expressed resistance (R) genes on wheat chromosomes. Irrespective the host or pest type, most 46 cloned R from 12 plant species share a strong sequence similarity, especially for protein domains motifs. By utilizing structural similarity perform modified RNA fingerprinting data mining, we identified 184 putative wheat. These include 87 NB/LRR types, 16 receptor-like kinases, 13 Pto-like kinases. remaining seven Hm1...
Abstract A total of 944 expressed sequence tags (ESTs) generated 2212 EST loci mapped to homoeologous group 1 chromosomes in hexaploid wheat (Triticum aestivum L.). deletion maps and the consensus map were constructed show distribution. unevenly distributed among 1A, 1B, 1D with 660, 826, 726, respectively. The number was greater on long arms than short for all three chromosomes. distribution ESTs along chromosome nonrandom clusters occurring distal regions middle arms. Duplications other...
Root and stem rot is one of the major diseases soybean. It caused by oomycete pathogen Phytophthora sojae. A series resistance genes (Rps) have been providing soybean with reasonable protection against this pathogen. Among these genes, Rps8, which confers to most P. sojae isolates, recently has mapped. However, closely linked molecular marker was mapped at about 10 cM from Rps8. In investigation, we attempted develop a high-density genetic map Rps8 region identify SSR markers for...
Scarcity of good-quality irrigation water is a major impediment to meet food demand for growing world population. Recycled waters may be available locally more affordably, but their higher salinity concern. Salinity effects on spinach mineral composition, antioxidant capacity, photosynthesis, and gene expression have not been established. Spinach cv. Raccoon was greenhouse-grown irrigated with four levels electrical conductivities (ECiw) 1.4 (control) or ranging from 3.6 9.4 dS m−1, combined...
Two spinach (Spinacea oleracea L.) cultivars were evaluated for their response to deficient (0.25 mmolc L−1 or 0.25 K) and sufficient (5.0 5.0 potassium (K) levels combined with salinities of 5, 30, 60, 90, 120 NaCl. Plants substituted K Na proportionally salinity within each dose. favored K+ over Na+, regardless salinity, accumulating significantly less at than K. Salinity had no effect on N, P, shoot accumulation, suggesting that plants can maintain NPK homeostasis even low soil Ca Mg...
Abstract We constructed high-density deletion bin maps of wheat chromosomes 5A, 5B, and 5D, including 2338 loci mapped with 1052 EST probes 217 previously (total 2555 loci). This information was combined to construct a consensus chromosome map group 5 24 bins. A relatively higher number were on 5B (38%) compared 5A (34%) 5D (28%). Differences in the levels polymorphism among three partially responsible for these differences. duplicated found (42%). Three times more long arms than short arms,...
Diminishing availability of non-saline water in arid and semiarid regions is concern to all irrigated agricultural producers, including wine grape producers. Grapes are not a salt tolerant crop producers often face the choice either limiting fresh application, using alternative saline waters or combination both. We examined tolerance effect restricted application on three purported rootstocks grafted Cabernet Sauvignon scion 4-year replicated field experiment. ANOVA indicated significant...
The Salt Overly Sensitive (SOS) pathway regulates intracellular sodium ion homeostasis as a salt‐stress response in plants. This involves three main genes designated SOS1 , SOS2 and SOS3 which are members of the Na + /H exchanger ( NHX ), CBL‐interacting protein kinase CIPK ) Calcineurin B‐like CBL gene families, respectively. To identify characterize SOS spinach Spinacia oleracea species Amaranthaceae family, we conducted genome‐wide identification phylogenetic analyses from four species,...
Guar is a commercially important legume crop known for guar gum. tolerant to various abiotic stresses, but the mechanisms involved in its salinity tolerance are not well established. This study aimed understand molecular of guar. RNA sequencing (RNA-Seq) was employed leaf and root transcriptomes salt-tolerant (Matador) salt-sensitive (PI 340261) genotypes under control salinity. Our analyses identified total 296,114 unigenes assembled from 527 million clean reads. Transcriptome analysis...
Abstract RNA-binding proteins (RBPs) play critical roles in posttranscriptional gene regulation. Current methods of systematically profiling RBPs plants have been predominantly limited to interacting with polyadenylated (poly(A)) RNAs. We developed a method called plant phase extraction (PPE), which yielded highly comprehensive proteome (RBPome), uncovering 2,517 from Arabidopsis (Arabidopsis thaliana) leaf and root samples diverse array domains. identified traditional that participate...
Abstract Salinity is a major abiotic stress factor that can significantly impact crop growth, and productivity. In response to salt stress, the plant Salt Overly Sensitive (SOS) signaling pathway regulates homeostasis of intracellular sodium ion concentration. The SOS1 , SOS2 SOS3 genes play critical roles in SOS pathway, which belongs members Na + /H exchanger ( NHX ), CBL‐interacting protein kinase CIPK calcineurin B‐like CBL ) gene families, respectively. this study, we performed...
Theobromine is an important quality component in tea plants (Camellia sinensis), which produced from 7-methylxanthine by theobromine synthase (CsTbS), the key rate-limiting enzyme biosynthetic pathway. Our transcriptomics and widely targeted metabolomics analyses suggested that CsMYB114 acted as a potential hub gene involved regulation of biosynthesis. The inhibition expression using antisense oligonucleotides (ASO) led to 70.21% reduction level leaves plant, verified involvement...