A5023384380- Hereditary Neurological Disorders
- Connexins and lens biology
- RNA regulation and disease
- Barrier Structure and Function Studies
- Peripheral Neuropathies and Disorders
- Kawasaki Disease and Coronary Complications
- Ferrocene Chemistry and Applications
- Ultrasound and Hyperthermia Applications
- Genetic Neurodegenerative Diseases
- Metal complexes synthesis and properties
- Coronary Artery Anomalies
- Ultrasound Imaging and Elastography
- Vasculitis and related conditions
- Nerve injury and regeneration
- DNA and Nucleic Acid Chemistry
- Cell Adhesion Molecules Research
- Vector-borne infectious diseases
- Traumatic Brain Injury and Neurovascular Disturbances
- Ion channel regulation and function
- Toxin Mechanisms and Immunotoxins
- Veterinary medicine and infectious diseases
- Spinal Dysraphism and Malformations
- Adipokines, Inflammation, and Metabolic Diseases
- Cyprus History, Politics, Society
- Organometallic Compounds Synthesis and Characterization
Cyprus Institute of Neurology and Genetics
2014-2024
Limassol General Hospital
2021-2023
University of Ioannina
2010-2019
Charcot-Marie-Tooth disease type 4C is the most common recessively inherited demyelinating neuropathy that results from loss of function mutations in SH3TC2 gene. Sh3tc2−/− mice represent a well characterized model developing early onset progressive peripheral with hypo- and demyelination, slowing nerve conduction velocities disturbed nodal architecture. The aim this project was to develop gene replacement therapy for treating rescue phenotype mouse model. We generated lentiviral vector...
Pelizaeus-Merzbacher-like disease or hypomyelinating leukodystrophy-2 is an autosomal recessively inherited leukodystrophy with childhood onset resulting from mutations in the gene encoding gap junction protein connexin 47 (Cx47, encoded by GJC2). Cx47 expressed specifically oligodendrocytes and crucial for junctional communication throughout central nervous system. Previous studies confirmed that a cell autonomous loss-of-function mechanism underlies transgenic oligodendrocyte-specific...
Type 4C Charcot-Marie-Tooth (CMT4C) demyelinating neuropathy is caused by autosomal recessive SH3TC2 gene mutations. highly expressed in myelinating Schwann cells. CMT4C a childhood-onset progressive disease without effective treatment. Here, we generated therapy for mediated an adeno-associated viral 9 vector (AAV9) to deliver the human Sh3tc2
Abstract Gap junctions (GJs) coupling oligodendrocytes to astrocytes and other are formed mainly by connexin47 (Cx47) a smaller portion connexin32 (Cx32). Mutations in both connexins cause inherited demyelinating disorders, but their expression is also disrupted multiple sclerosis (MS). To clarify whether the loss of either Cx47 or Cx32 could modify outcome inflammation myelin loss, we induced experimental autoimmune encephalomyelitis (EAE) fully backcrossed knockout (KO) Cx47KO mice...
Glial gap junction proteins, called connexins (Cxs), form junctions in the central nervous system to allow bidirectional cytosolic exchange of molecules between adjacent cells. Their involvement inheritable diseases and use experimental animal models that closely mimic such revealed critical role glial GJs myelination homeostasis. Cxs are also implicated acquired demyelinating disorders, as Multiple Sclerosis (MS) Alzheimer's disease (AD). Animal human studies have a astrocytic Cx43...
Induction of experimental autoimmune encephalomyelitis (EAE), an animal model multiple sclerosis (MS), in connexin 32 (Cx32) or Cx47 knockout (KO) mice with deficiency oligodendrocyte gap junctions (GJs) results a more severe disease course. In particular, KO EAE experience earlier onset and pronounced severity, accompanied by dysregulated pro-inflammatory responses preceding the manifestations. this study, analysis relevant cytokines wild type EAE, Cx32 revealed altered expression Vcam-1 [7...
Abstract This article describes a step‐by‐step process of lumbar intrathecal injection Evans blue dye and AAV9‐EGFP in adult (2‐month‐old) neonatal (postnatal day 10) mice. Intrathecal is clinically translatable technique that has already been extensively applied humans. In mice, considered challenging procedure requires trained experienced researcher. For both directed into the L5‐L6 intervertebral space. Intrathecally injected material enters cerebrospinal fluid (CSF) within space from...
Focussed Ultrasound (FUS) combined with microbubbles (MBs) was proven a promising modality for non-invasive blood brain barrier disruption (BBBD). Herein, two devices FUS-mediated BBBD in rodents are presented.A two-axes robotic device manufactured navigating single element FUS transducer of 1 MHz relative to the rodents. A second more compact featuring motorized vertical axis also developed. Their performance assessed terms motion accuracy, MRI compatibility and trans-skull wild type mice...
Mitofusin 1 (MFN1) and MFN2 are outer mitochondrial membrane fusogenic proteins regulating network morphology. mutations cause Charcot-Marie-Tooth type 2A (CMT2A), an axonal neuropathy characterized by fusion defects, which in the case of a GTPase domain mutant, were rescued following wild-type MFN1/2 (MFN1/2WT ) overexpression. In this study, we compared therapeutic efficiency between MFN1WT MFN2WT overexpression correcting defects induced novel MFN2K357T mutation located highly conserved...
Four mixed ligand gold(I) complexes with the thioamides 2-mercapto-thiazolidine (mtzdH), 2-mercapto-benzothiazole (mbztH) and 5-chloro-2-mercapto-benzothiazole (ClmbztH) triphenylphosphine (tpp) of formulae [Au(tpp)Cl] (1) [Au(tpp)(mtzd)] (2), [Au(tpp)(mbzt)] (3) [Au(tpp)(Clmbzt)] (4), already known, were used to study their mechanism inhibition activity towards catalytic oxidation linoleic acid hydroperoxylinoleic by enzyme lipoxygenase (LOX), kinetically theoretically. The results are...
Abstract Kawasaki disease (KD) is one of the most common vasculitides early childhood. There are no previous studies on KD in Cyprus. The aim this study was to evaluate epidemiology Cyprus, risk factors for resistance treatment and development cardiac complications. This a retrospective multicenter pediatric patients with hospitalized between January 2000 and-December 2019. data were collected from medical records. A total 136 included study. 83% < 5 years age 10% 6 months. Thirty (22%)...
Amyloid-β (Aβ) peptides, the main component of amyloid plaques found in Alzheimer's disease (AD) brain, are implicated its pathogenesis, and considered a key target AD therapeutics. We herein propose reliable strategy for non-invasively delivering specific anti-Aβ antibody mouse model by microbubbles-enhanced Focused Ultrasound (FUS)-mediated Blood-brain barrier disruption (BBBD), using simple single stage MR-compatible positioning device. The initial experimental work involved wild-type...
Endothelin alters central sympathetic responses, but the resultant effects on arrhythmogenesis are unknown. We examined ventricular tachyarrhythmias after endothelin receptor-A blockade in brain of Wistar rats with acute myocardial infarction. For this aim, BQ-123 (n=6) or phosphate-buffered saline were injected intracerebroventricularly. After 10 min, left coronary artery was ligated, followed by implantation telemetry transmitters. Electrocardiography and voluntary activity (as a surrogate...
A Rare Case of Tamoxifen-Induced ThrombocytopeniaAnurag Pathak, Sushil Kiran Kunder, Avinash, Navin Patil, N Karthik Rao
Abstract Background: Kawasaki disease (KD) is one of the most common vasculitides early childhood and first cause acquired heart in developed countries. There are no previous studies on KD Cyprus. The aim this study was to evaluate epidemiology Cyprus, risk factors for resistance treatment development cardiac complications. Lastly, Kobayashi Egami scoring systems were evaluated our population. Methods: This a retrospective multicenter pediatric patients with hospitalized between January 2000...