It is well-known that speckle a multiplicative noise degrades the visual evaluation in ultrasound imaging. The recent advancements instrumentation and portable devices necessitate need of more robust despeckling techniques for enhanced medical imaging both routine clinical practice teleconsultation. objective this work was to carry out comparative despeckle filtering based on texture analysis, image quality metrics, by experts assessment 440 (220 asymptomatic 220 symptomatic) images carotid...

There are indications that the morphology of atherosclerotic carotid plaques, obtained by high-resolution ultrasound imaging, has prognostic implications. The objective this study was to develop a computer-aided system will facilitate characterization plaques for identification individuals with asymptomatic stenosis at risk stroke. A total 230 plaque images were collected which classified into two types: symptomatic because ipsilateral hemispheric symptoms, or they not connected events. Ten...

To what extent does a trophectoderm (TE) biopsy reliably reflect the chromosomal constitution of inner cell mass (ICM) in human blastocysts?Concordance between TE and ICM was established 62.1% embryos analysed.Next generation sequencing (NGS) platforms have recently been optimised for preimplantation genetic testing aneuploidies (PGT-A). However, higher sensitivity has led to an increase reports mosaicism within single biopsy. This raised substantial controversy surrounding prevalence...

The shapes and firing rates of motor unit action potentials (MUAPs) in an electromyographic (EMG) signal provide important source information for the diagnosis neuromuscular disorders. In order to extract this from EMG signals recorded at low moderate force levels, it is required: i) identify MUAPs composing signal, ii) classify with similar shape, iii) decompose superimposed MUAP waveforms into their constituent MUAPs. For classification two different pattern recognition techniques are...

This study reports the epidemiology of respiratory syncytial virus (RSV) in hospitalized children Cyprus over three successive seasons (2010-2013) and association between prevalent genotypes disease severity. RSV infections had a circulation pattern from December to March. Most RSV-positive (83%) were aged <2 years. Genotyping isolates showed that during first winter season (2010-2011), only genotype circulating was GA2 (RSV-A), followed by BA (RSV-B) next with few sporadic cases GA2. During...

Charcot-Marie-Tooth disease type 4C is the most common recessively inherited demyelinating neuropathy that results from loss of function mutations in SH3TC2 gene. Sh3tc2−/− mice represent a well characterized model developing early onset progressive peripheral with hypo- and demyelination, slowing nerve conduction velocities disturbed nodal architecture. The aim this project was to develop gene replacement therapy for treating rescue phenotype mouse model. We generated lentiviral vector...

Abstract Mutations in the GJB1 gene, encoding gap junction (GJ) protein connexin32 (Cx32), cause X-linked Charcot-Marie-Tooth disease (CMT1X), an inherited demyelinating neuropathy. We developed a gene therapy approach for CMT1X using AAV9 vector to deliver GJB1/Cx32 under myelin zero ( Mpz ) promoter targeted expression Schwann cells. Lumbar intrathecal injection of -Mpz.GJB1 resulted widespread biodistribution peripheral nervous system including lumbar roots, sciatic and femoral nerves, as...

Charcot-Marie-Tooth disease type 1A (CMT1A), the most common inherited demyelinating peripheral neuropathy, is caused by PMP22 gene duplication. Overexpression of WT in Schwann cells destabilizes myelin sheath, leading to demyelination and ultimately secondary axonal loss disability. No treatments currently exist that modify course. The direct route CMT1A therapy will involve reducing normal levels. To accomplish this, we developed a strategy reduce using artificial miRNAs targeting human...

Poliovirus type 1 neurovirulence is difficult to analyze because of the 56 mutations which differentiate neurovirulent Mahoney strain from attenuated Sabin strain. We have isolated four mutants differ temperature-sensitive parental by only a few mutations, using selection for temperature resistance: mutant S(1)37C1 was at 37.5 degrees C, S(1)38C5 38.5 and S(1)39C6 S(1)39C10 were 39.5 C. All had positive reproductive capacity supraoptimal (Rct+ phenotype). Mutant induced paralysis in two...

Two human neuroblastoma cell lines were persistently infected with poliovirus strains of all three serotypes. In IMR-32 cells, which studied in greatest detail, viral antigens present most and over a 9-month period virions found the medium at high titers. Persistently cells resistant to superinfection by Sabin 1, 2, 3 but sensitive coxsackievirus B3. The viruses recovered from for conservation epitopes, host specificity, temperature resistance phenotype. antigenic site 1 carried major capsid...

In order to improve clinical management and prevention of viral infections in hospitalised children improved etiological insight is needed. The aim the present study was assess spectrum respiratory pathogens admitted hospital with acute tract Cyprus. For this purpose nasopharyngeal swab samples from 424 less than 12 years age were collected over three epidemic seasons analysed for presence most common 15 viruses. A pathogen identified 86% samples, multiple being observed almost 20% samples....

Orally administered compounds represent the great majority of all pharmaceutical produced for human use and are most popular among patients since they practical easy to self-administer. Following ingestion, orally drugs begin a “perilous” journey down gastrointestinal tract their bioavailability is modulated by numerous factors. The (GI) anatomy can modulate drug accounts interpatient response heterogeneity. Furthermore, host genetics contributor modulation. Importantly, component GI that...

X-linked Charcot-Marie-Tooth disease (CMT1X) is an inherited demyelinating neuropathy caused by loss-of-function mutations in the GJB1 gene, encoding gap junction protein connexin32 (Cx32). Cx32 plays a critical role Schwann cell function and myelin formation peripheral nervous system. We have developed gene replacement therapeutic approach using humanized AAVrh10 vector construct expressing under control of cell-specific human zero (MPZ) promoter. Lumbar intrathecal injection increasing...

The COVID-19 pandemic has had a profound impact on healthcare systems world-wide, with severe consequences the global economy and society. clinical presentation of SARS-CoV-2 infection varies widely, ranging from asymptomatic cases to disease death. Coinfection other respiratory pathogens in SARS-CoV-2-positive individuals may exacerbate symptom severity lead poorer outcomes. Background/Objectives: This study is first investigate prevalence viral bacterial co-infections Cyprus. Methods: A...

The aim of this work was to develop a system based on multifeature texture analysis and modular neural networks that will facilitate the automated interpretation satellite cloud images. Such provide standardized efficient way for classifying types can be used as an operational tool in weather analysis. A series 98 infrared images from geostationary METEOSAT7 were employed, 366 segments labeled into six after combined agreed observations ground satellite. From segmented images, nine different...

Pelizaeus-Merzbacher-like disease or hypomyelinating leukodystrophy-2 is an autosomal recessively inherited leukodystrophy with childhood onset resulting from mutations in the gene encoding gap junction protein connexin 47 (Cx47, encoded by GJC2). Cx47 expressed specifically oligodendrocytes and crucial for junctional communication throughout central nervous system. Previous studies confirmed that a cell autonomous loss-of-function mechanism underlies transgenic oligodendrocyte-specific...

Gap junction beta-1 (GJB1) gene mutations affecting the gap protein connexin32 (Cx32) cause X-linked Charcot-Marie-Tooth disease (CMT1X), a common inherited neuropathy. Targeted expression of virally delivered Cx32 in Schwann cells following intrathecal injection lentiviral vectors knockout (KO) mouse model has led to morphological and functional improvement. To examine whether this approach could be effective CMT1X patients expressing different mutants, we treated transgenic KO mice T55I,...

X-linked Charcot-Marie-Tooth disease (CMT1X) is a common inherited neuropathy caused by mutations in the GJB1 gene encoding gap junction protein connexin32 (Cx32). Clinical studies and models indicate that mainly results from Schwann cell autonomous, loss-of-function mechanisms; therefore, CMT1X may be treatable replacement.A lentiviral vector LV.Mpz-GJB1 carrying under cell-specific myelin zero (Mpz) promoter was generated delivered into mouse sciatic nerve single injection immediately...

X-linked Charcot-Marie-Tooth disease (CMT1X), one of the commonest forms inherited demyelinating neuropathy, results from GJB1 gene mutations causing loss function gap junction protein connexin32 (Cx32). The aim this study was to examine whether delayed replacement therapy after onset peripheral neuropathy can provide a therapeutic benefit in Gjb1-null/Cx32 knockout model CMT1X. After delivery LV-Mpz.GJB1 lentiviral vector by single lumbar intrathecal injection into 6-month-old Gjb1-null...

Human enteroviruses are responsible for a wide spectrum of clinical diseases affecting many different organ systems. Although infection is usually asymptomatic, infections the central nervous system manifested as meningitis or encephalitis can pose serious public health problem, especially during outbreaks. In this study, samples from 218 patients diagnosed with enteroviral between January 2000 and December 2002 were analysed in order to assess epidemiology human cause viral Cyprus. A new...

The aim of this study was to gain insights into the tempo and mode evolutionary processes that sustain genetic diversity in coxsackievirus B5 (CVB5) interplay with virus transmission. We estimated phylodynamic patterns a large sample strains collected Europe by Bayesian statistical methods, reconstructed ancestral states genealogical nodes, tested for selection. genealogies structural one-dimensional gene encoding VP1 protein nonstructural 3CD locus allowed precise description lineages over...

Type 4C Charcot-Marie-Tooth (CMT4C) demyelinating neuropathy is caused by autosomal recessive SH3TC2 gene mutations. highly expressed in myelinating Schwann cells. CMT4C a childhood-onset progressive disease without effective treatment. Here, we generated therapy for mediated an adeno-associated viral 9 vector (AAV9) to deliver the human Sh3tc2

Human papillomavirus (HPV) is the most common sexually transmitted agent, and it can cause cervical lesions cancer in females. Currently, information regarding prevalence of HPV Cyprus lacking. The aim this study was to evaluate type-specific 596 women, aged 19–65 years, with cytological abnormalities. Additionally, a subset 348 women for whom cytology results Pap test were available, association between infection disease investigated. detection typing carried out using PCR restriction...