A5103203386- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Congenital heart defects research
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
Lawrence Berkeley National Laboratory
2021
University of California, San Francisco
2020
Cardiac pacemaker cells (PCs) in the sinoatrial node (SAN) have a distinct gene expression program that allows them to fire automatically and initiate heartbeat. Although critical SAN transcription factors, including Isl1 (Islet-1), Tbx3 (T-box factor 3), Shox2 (short-stature homeobox protein 2), been identified, cis-regulatory architecture governs PC-specific is not understood, discrete enhancers required for regulation identified.To define epigenetic profile of PCs using comparative...
The safety of CRISPR-based gene editing methods is the utmost priority in clinical applications. Previous studies have reported that Cas9 cleavage induced frequent aneuploidy primary human T cells, but whether cleavage-mediated base editors would generate off-target structure variations remains unknown. Here, we investigate potential structural associated with CRISPR/Cas9, ABE, and CBE mouse embryos cells by whole-genome sequencing single-cell RNA-seq analyses.