A5037598219- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Plant pathogens and resistance mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Resistance
- Bacteriophages and microbial interactions
- Toxin Mechanisms and Immunotoxins
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
John Innes Centre
2017-2023
Norwich Research Park
2017-2023
Abstract The ability to recombinantly produce target proteins is essential many biochemical, structural, and biophysical assays that allow for interrogation of molecular mechanisms behind protein function. Purification solubility tags are routinely used maximise the yield ease expression purification from E. coli . A major hurdle in high-throughput trials cloning required multiple constructs with different tags. Here we report a modification well-established pOPIN vector suite be compatible...
Plant pathogens manipulate the cellular environment of host to facilitate infection and colonization that often lead plant diseases. To accomplish this, many specialized secrete virulence proteins called effectors into cell, which subvert processes such as immune signaling, gene transcription, metabolism. Phytophthora infestans, causative agent potato late blight, employs an expanded repertoire RxLR with WY domains through direct interaction protein targets. However, our understanding...
A diversity of plant-associated organisms secrete effectors—proteins and metabolites that modulate plant physiology to favor host infection colonization. However, effectors can also activate immune receptors, notably nucleotide-binding domain leucine-rich repeat-containing (NLR) proteins, enabling plants fight off invading organisms. This interplay between effectors, their targets, the matching receptors is shaped by intricate molecular mechanisms exceptionally dynamic coevolution. In this...
ABSTRACT Unconventional integrated domains in plant intracellular immune receptors (NLRs) can directly bind translocated pathogen effector proteins to initiate an response. The rice receptor pairs Pik-1/Pik-2 and RGA5/RGA4 both use heavy metal-associated (HMA) the Magnaporthe oryzae effectors AVR-Pik AVR-Pia, respectively. These belong MAX family share a core structural fold, despite being divergent sequence. How maintain specificity of recognition, even for structurally similar effectors,...
Abstract Plant pathogens manipulate the cellular environment of host to facilitate infection and colonization, often leading plant diseases. To accomplish this, many specialized secrete virulence proteins called effectors into cell, which subvert processes such as immune signalling, gene transcription, metabolism. Phytophthora infestans , causative agent potato late blight, employs an expanded repertoire RxLR with WY domains through direct interaction protein targets. However, our...