A5040598848- Acute Myeloid Leukemia Research
- Hematological disorders and diagnostics
- Pesticide Exposure and Toxicity
- Hematopoietic Stem Cell Transplantation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Occupational and environmental lung diseases
- Digital Imaging for Blood Diseases
- Bone and Joint Diseases
- Cancer, Hypoxia, and Metabolism
- Carcinogens and Genotoxicity Assessment
- Human Health and Disease
- Hemoglobinopathies and Related Disorders
- Genomics, phytochemicals, and oxidative stress
- Lymphoma Diagnosis and Treatment
- High Altitude and Hypoxia
- Cancer-related molecular mechanisms research
- Retinoids in leukemia and cellular processes
- T-cell and B-cell Immunology
- Chronic Myeloid Leukemia Treatments
- Fatty Acid Research and Health
- Multiple Sclerosis Research Studies
- Autoimmune and Inflammatory Disorders Research
- Healthcare and Venom Research
- Metabolism and Genetic Disorders
- Cardiac Arrhythmias and Treatments
Université de Tours
2017-2024
Inserm
2024
Centre National de la Recherche Scientifique
2018-2023
Niche leucémique et métabolisme oxydatif
2018-2023
Centre Hospitalier Universitaire de Tours
2017-2021
Abstract Clonal hematopoiesis (CH) of indeterminate potential has been described in blood samples from large series patients. Its prevalence and consequences are still not well understood because sequencing methods vary most studies were performed cohorts comprising individuals with nonhematologic diseases. Here, we investigated the frequency CH 82 paired bone marrow carefully selected healthy adult volunteers. Forty-one genes known to be mutated myeloid malignancies sequenced a 1% threshold...
Abstract The bone marrow (BM) niche impacts the progression of acute myeloid leukemia (AML) by favoring chemoresistance AML cells. Intimate interactions between leukemic cells and BM mesenchymal stromal (BM-MSCs) play key roles in this process. Direct intercellular communications hematopoietic BM-MSCs involve connexins, components gap junctions. We postulated that blocking junction assembly could modify cell–cell consequently chemoresistance. comparison from patients healthy donors revealed...
Abstract Myelodysplastic syndromes (MDS) are a heterogeneous group of clonal stem cell disorders with an inherent tendency for transformation in secondary acute myeloid leukemia. This study focused on the redox metabolism bone marrow (BM) cells from 97 patients compared 25 healthy controls. The level reactive oxygen species (ROS) was quantified by flow cytometry BM subsets as well expression 28 transcripts encoding major enzymes involved antioxidant cellular response. Our results highlight...
The incidence of myelodysplastic syndrome increases with aging and the early diagnosis enables optimal care these diseases. DxH 800 hematology analyzer measures calculates 126 cytological parameters, but only 23 are used for routine CBC assessment. goal this study was to use 103 unexploited “research parameters” develop an algorithm allowing detection subclinical MDS patients by triggering morphological analysis. Blood sample parameters from 101 88 healthy volunteers were analyzed identify...
The bone marrow (BM) microenvironment plays a crucial role in the development and progression of leukemia (AML). Intracellular reactive oxygen species (ROS) are involved regulation biology leukemia-initiating cells, where antioxidant enzyme GPx-3 could be as determinant cellular self-renewal. Little is known however about control oxidative metabolism AML cells. In present study, coculture model BM mesenchymal stromal cells (MSCs) (KG1a cell-line primary blasts) was used to explore this...
(1) Background: The impact of occupational exposure to high doses pesticides on hematologic disorders is widely studied. Yet, lifelong low pesticides, and more particularly their cocktail effect, although poorly known, could also participate the development such hematological diseases as myelodysplastic syndrome (MDS) in elderly patients. (2) Methods: In this study, a seven frequently present water food (maneb, mancozeb, iprodione, imazalil, chlorpyrifos ethyl, diazinon dimethoate),...
Abstract Low reactive oxygen species (ROS) levels are well‐established characteristics of mouse hematopoietic stem cells (HSCs). However, little is known about these in human HSCs. This study aimed at quantifying ROS CD34 + CD38 low and high progenitors from bone marrow, cord blood mobilized for autologous HSC transplantation. A specifically devised multiparameter flow cytometry method was used to quantify (H 2 DCFDA staining) sub‐populations primary cells. Results were confirmed by...
In ineffective erythropoiesis, hepcidin synthesis is suppressed by erythroid regulators, namely erythroferrone and growth differentiation factor-15. For the first time, hypothesis that iron overload in megaloblastic anemia may be related to erythropoiesis explored describing kinetics of hepcidin, erythroferrone, factor-15 levels a patient diagnosed with associated overload.An 81-year-old Caucasian male was admitted for fatigue. He had type-2 diabetes previously treated metformin, ischemic...
Left ventricle ejection fraction (LVEF) ≤ 30–35% is widely accepted as a cut-off for primary prevention with an implantable cardiac defibrillator (ICD) in patients both ischaemic and non-ischaemic cardiomyopathy supposedly on optimal medical therapy. This study reports evolutions of LVEF treatments implanted our institution ICD sudden death, after 2 years follow-up. Among 84 under 35% between 2005 2007, 28 (33%) had improved their >35% the During this period, even if Beta-blockers (98%)...
Background: Moderate anemia affects a substantial fraction of the elderly population and blood smear analysis can help to guide towards diagnosis myelodysplasia (MDS). Nevertheless, in medical laboratories, review is realized only if there quantitative or qualitative flag on complete count (CBC). Consequently, suspicion MDS could be delayed because absence systematic triggering. The DxH 800 Hematology Analyzer measures calculates 126 cytological parameters based photometry, impedance light...
Background: In the context of myelodysplasias (MDS) and acute mydeloid leukemias (AML), discovery in hematopoietic cells aberrant DNA methylation profiles mutations genes associated with regulation has allowed for development demethylating agents. However, such treatments affect whole bone marrow (BM), including mesenchymal stromal (MSCs), epigenetic landscape which is poorly known. Aims: Considering importance BM‐MSCs pathophysiology MDS AML, aim this study was to compare MSCs from AML...
Background: The deleterious effects of occupational pesticide exposure (OPE) in human health are a major public concern. Several studies showed the association between OPE and lymphohematopoietic malignancies. Association acute myeloid leukemias (AML) is not clearly demonstrated published due to heterogeneous cohorts without control groups, which AML pooled with other (chronic leukemia and/or chronic lymphoid leukemia). Aims: Our objective was realize stringent meta-analysis case-control on...
Background: Bone marrow niche of acute myeloid leukemia (AML) regulates leukemic progression by favoring cell quiescence and chemoresistance. Niche components, especially mesenchymal stromal cells (MSCs) protect from chemotherapy‐induced apoptosis. Direct intercellular communications between hematopoietic stem MSCs involve connexins, key components gap junctions, which could be interresting targets to reverse this phenomenom. Aims: We postulated that blocking the assembly connexins modify...
7540 Background: Occupational pesticide exposure (OPE) is associated with the risk of developing lymphoid malignancies, but less information available on large cohorts about occurrence acute myeloid leukemia (AML). To answer this question, we performed a meta-analysis including relevant adult case-control studies which reported OPE. Methods: Following PRISMA and MOOSE guidelines, two investigators systematic search in PubMed Cochrane databases for evaluating association between OPE AML 1946...