A5082792260- Autophagy in Disease and Therapy
- Calcium signaling and nucleotide metabolism
- Metabolism, Diabetes, and Cancer
- Graphene and Nanomaterials Applications
- Phytochemistry and Biological Activities
- Parkinson's Disease Mechanisms and Treatments
- Computational Drug Discovery Methods
- Advanced Nanomaterials in Catalysis
- Retinoids in leukemia and cellular processes
- Genomics, phytochemicals, and oxidative stress
- Cancer-related Molecular Pathways
- Natural product bioactivities and synthesis
- Endodontics and Root Canal Treatments
- Pancreatic function and diabetes
- Cholinesterase and Neurodegenerative Diseases
- Carbon and Quantum Dots Applications
- Inflammasome and immune disorders
- Surface Chemistry and Catalysis
- Heme Oxygenase-1 and Carbon Monoxide
- Ginseng Biological Effects and Applications
- Alkaloids: synthesis and pharmacology
- Random Matrices and Applications
- Dental Radiography and Imaging
- Ubiquitin and proteasome pathways
- Nonlinear Optical Materials Studies
University of Belgrade
2014-2025
Institute for Biological Research “Siniša Stanković”
2016-2021
Institute for Biological Sciences
2017
Endothelial dysfunction is an early pathophysiological event in atherosclerosis. The endothelial-protective abilities of diethylether extract (EE) from the Gentiana kochiana (Gentianaceae) herb and its main component, xanthone aglycone gentiacaulein (GC), were evaluated oxidized low-density lipoprotein (oxLDL)-treated EA.hy926 endothelial cell line. EE GC actions assessed using viability assays, flow cytometry, immunoblot, DPPH, NBT, TBARS, conjugated diene formation, Griess tests. Both...
We investigated the ability of ascorbic acid (AA) and menadione (MD) combination, well-known reactive oxidative species- (ROS-) generating system, to induce autophagy in human U251 glioblastoma cells. A combination AA MD (AA+MD), contrast single treatments, induced necrosis-like cell death mediated by mitochondrial membrane depolarization extremely high stress. AA+MD, a lesser extent alone, prompted appearance markers such as autophagic vacuoles, autophagosome-associated LC3-II protein,...
We investigated the cytotoxicity of recently synthesized (S,S)-ethylendiamine-N,N'-di-2-(3-cyclohexyl)propanoic acid esters toward human leukemic cell lines and healthy blood mononuclear cells. Cell viability was assessed by phosphatase assay, apoptosis, differentiation were analyzed flow cytometry electron microscopy, while intracellular localization apoptosis-inducing factor (AIF) determined immunoblotting. It demonstrated that methyl, ethyl, n-propyl toxic to HL-60, REH, MOLT-4, KG-1,...
We explored the interplay between intracellular energy sensor AMP-activated protein kinase (AMPK), extracellular signal-regulated (ERK), and autophagy in phorbol myristate acetate (PMA)-induced neuronal differentiation of SH-SY5Y human neuroblastoma cells. PMA-triggered expression markers (dopamine transporter, microtubule-associated 2, β-tubulin) was associated with an autophagic response, measured by conversion light chain 3 (LC3)-I to autophagosome-bound LC3-II, increase flux,...
Thirteen novel tacrine derivatives were designed and synthesized consisting of N-aryl-piperidine-4-carboxamide moiety connected by a five-methylene group linker. An anti-Alzheimer disease (AD) potential newly was evaluated against two important AD targets, acetylcholinesterase (AChE) butyrylcholinesterase (BuChE). In vitro pharmacological evaluation showed strong ChE inhibitory activity all compounds, with IC50 values ranging from 117.5 to 455 nM for AChE 34 324 BuChE. As representative the...
Abstract The protective ability of novel arylpiperazine‐based dopaminergic ligands against nitric oxide (NO)‐mediated neurotoxicity is investigated. most potent neuroprotective arylpiperazine identified during the study was N ‐{4‐[2‐(4‐phenyl‐piperazin‐1‐yl)ethyl]‐phenyl}picolinamide, which protected SH‐SY5Y human neuron‐like cells from proapoptotic effect NO donor sodium nitroprusside (SNP) by decreasing oxidative stress, mitochondrial membrane depolarization, caspase activation and...
Melanoma, an aggressive skin tumor with high metastatic potential, is associated mortality and increasing morbidity. Multiple available chemotherapeutic immunotherapeutic modalities failed to improve survival in advanced disease, the search for new agents ongoing. The aim of this study was investigate antimelanoma effects O , -diethyl-( S )-ethylenediamine- N ′di-2-(3-cyclohexyl) propanoate dihydrochloride (EE), a previously synthesized characterized organic compound. Mouse melanoma B16 cell...
Numerous beneficial effects of food restriction on aging and age-related pathologies are well documented. It is also well-established that both short- long-term regimens induce elevated circulating levels glucocorticoids, stress-induced hormones produced by adrenal glands can exert deleterious the brain. In present study, we examined effect glucocorticoid hormone/glucocorticoid receptor (GR) system in cortex during aging, 18- 24-month-old rats. Corticosterone level was increased aged ad...
Introduction: The accumulation of alpha-synuclein (ASYN) in susceptible neurons is considered to be a major contributing factor pathogenesis Parkinson's disease. Although ASYN was as an intracellular protein, recent data suggest that it can detected extracellularly. Autophagy plays important role degradation; therefore, impairment autophagy could contributor accumulation. ATG (autophagy-related genes) proteins function at several physiologically continuous steps autophagy, and ATG7 essential...
Glioblastoma multiforme (GBM) is the most lethal primary brain tumor in adults, characterized by a highly invasive nature and therapy resistance. Combination of menadione ascorbic acid (AA+MD) exerts strong ROS-mediated anti-GBM activity vitro. The objective this study was to improve AA+MD potential modulating Akt c-Jun N-terminal kinase (JNK), molecules with an important role GBM development. effects JNK modulation on toxicity U251 human glioblastoma cells were assessed cell viability...