A5035189635- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Pancreatic and Hepatic Oncology Research
- Inflammasome and immune disorders
- Cancer Genomics and Diagnostics
- Inflammatory Biomarkers in Disease Prognosis
- Neuroendocrine Tumor Research Advances
- Mast cells and histamine
- Immune Cell Function and Interaction
- Lung Cancer Treatments and Mutations
- Neuroblastoma Research and Treatments
- HER2/EGFR in Cancer Research
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- CRISPR and Genetic Engineering
- melanin and skin pigmentation
- Ubiquitin and proteasome pathways
- Multiple Myeloma Research and Treatments
- Pancreatitis Pathology and Treatment
- Cancer Research and Treatments
- Lung Cancer Research Studies
- Cutaneous Melanoma Detection and Management
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2015-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2015-2025
Vita-Salute San Raffaele University
2015-2025
IRCCS Ospedale San Raffaele
2022-2023
Bipar
2015-2019
San Raffaele University of Rome
2011-2017
Abstract In pancreatic ductal adenocarcinomas (PDAC), lymphoid infiltrates, comprised mainly of Th2 cells, predict a poor survival outcome in patients. IL4 signaling has been suggested to stabilize the phenotype this setting, but cellular source PDAC is unclear. Here, we show that basophils expressing are enriched tumor-draining lymph nodes (TDLN) Basophils present TDLNs correlated significantly with Th2/Th1 cell ratio tumors, where they served as an independent prognostic biomarker patient...
<h3>Background</h3> The thymic stromal lymphopoietin (TSLP), a key cytokine for development of Th2 immunity, is produced by cancer associated fibroblasts (CAFs) in pancreatic where predominant tumor infiltrating over Th1 cells correlates with reduced patients' survival. Which and molecules are mostly relevant driving TSLP secretion CAFs not defined. <h3>Methods</h3> We performed vitro, vivo ex-vivo analyses. For vitro studies we used cell lines, primary cultures, THP1 cells. was as read-out...
The ability to identify the broadest range of targetable gene fusions is crucial facilitate personalized therapy selection for advanced lung adenocarcinoma (LuADs) patients harboring receptor tyrosine kinase (RTK) genomic alterations. In order evaluate most effective testing approach LuAD fusion detection, we analyzed 210 NSCLC selected clinical samples, comparing in situ (Fluorescence Situ Hybridization, FISH, and ImmunoHistoChemistry, IHC) molecular (targeted RNA Next-Generation...
There is increased production of plasmacytoid dendritic cells (pDCs) in the bone marrow (BM) multiple myeloma (MM) patients and these favor Th22 cell differentiation. Here, we found that frequency interleukin (IL)-22+IL-17−IL-13+ T significantly peripheral blood (PB) BM stage III relapsed/refractory MM compared with healthy donors asymptomatic or I/II disease. cloned from were CCR6+CXCR4+CCR4+CCR10− produced IL-22 IL-13 but not IL-17. Furthermore, polyfunctional Th22-Th2 Th22-Th1 clones...
Assessment of HRD status is now essential for ovarian cancer patient management. A relevant percentage high-grade serous carcinoma (HGSC) characterized by HRD, which caused genetic alterations in the homologous recombination repair (HRR) pathway. Recent trials have shown that not only patients with pathogenic/likely pathogenic BRCA variants, but also BRCAwt/HRD patients, are sensitive to PARPis and platinum therapy. The most common test Myriad MyChoice CDx, there a pressing need offer an...
The CDC25A-CDK2 pathway has been proposed as critical for the oncogenic action of human epidermal growth factor receptor 2 (HER2) in mammary epithelial cells. In particular, transgenic expression CDC25A cooperates with HER2 promoting tumors, whereas hemizygous loss attenuates HER2-induced tumorigenesis penetrance. On basis this evidence a synergism between and cell cycle regulator mouse model tumorigenesis, we investigated role HER2-positive breast cancer its possible implications...
Introduction Despite predicted efficacy, immunotherapy in epithelial ovarian cancer (EOC) has limited clinical benefit and the prognosis of patients remains poor. There is thus a strong need for better identifying local immune dynamics immune-suppressive pathways limiting T-cell mediated anti-tumor immunity. Methods In this observational study we analyzed by immunohistochemistry, gene expression profiling flow cytometry antigenic landscape composition 48 EOC specimens, with focus on...
Abstract Adoptive transfer of CAR T cells demonstrated impressive results against B-cell malignancies, but still limited efficacy solid tumors. In this context, multiple challenges need to be overcome, including poor tumor recognition and strong immunosuppression within the microenvironment (TME). Our Unit has recently reported that pharmacological inhibition N-glycan synthesis in cancer increases cell by improving preventing exhaustion. project, we investigated role N-glycosylation blockade...
Abstract Pancreatic Ductal AdenoCarcinoma (PDAC) is a very aggressive disease, characterized by desmoplasia and Th2-inflammation. Th2-inflammation correlates with poor prognosis it driven the release of Thymic Stromal LymphoPoietin (TSLP) from Cancer Associated Fibroblasts (CAFs) activated tumor-derived pro-inflammatory cytokines. The aims our study are: i) to verify which cytokines are responsible for CAFs activation, ii) understand stimuli/mechanisms cytokine production in pancreatic...
Abstract HER2 gene amplification/overexpression has been identified in 10-34% of invasive breast cancers and correlates with reduced disease-free overall survival. The HER2-targeted therapy trastuzumab decreases the risk relapse prolongs Resistance to is a significant barrier effective HER2-positive cancer treatment: response rate ranges from 20-35%, underlining that hardly homogeneous disease and, more importantly, other HER2-related pathways need be unveiled. Recent studies provided...
<div>Abstract<p>In pancreatic ductal adenocarcinomas (PDAC), lymphoid infiltrates, comprised mainly of Th2 cells, predict a poor survival outcome in patients. IL4 signaling has been suggested to stabilize the phenotype this setting, but cellular source PDAC is unclear. Here, we show that basophils expressing <i>IL4</i> are enriched tumor-draining lymph nodes (TDLN) Basophils present TDLNs correlated significantly with Th2/Th1 cell ratio tumors, where they served as an...
<p>Expression of CCL24/eotaxin-2 in CAF supernatant-conditioned monocytes and TDLNs.</p>
<p>Expression of CCL24/eotaxin-2 in CAF supernatant-conditioned monocytes and TDLNs.</p>
<div>Abstract<p>In pancreatic ductal adenocarcinomas (PDAC), lymphoid infiltrates, comprised mainly of Th2 cells, predict a poor survival outcome in patients. IL4 signaling has been suggested to stabilize the phenotype this setting, but cellular source PDAC is unclear. Here, we show that basophils expressing <i>IL4</i> are enriched tumor-draining lymph nodes (TDLN) Basophils present TDLNs correlated significantly with Th2/Th1 cell ratio tumors, where they served as an...
Abstract Pancreatic neuroendocrine tumors (PanNETs) are the second most common malignancy of pancreas, and only curative option is represented by surgery. PanNETs can be classified as well- or poorly- differentiated, based on histology. Poorly-differentiated carcinomas (NECs) represent less than 10% cases, but they highly malignant associated with poor prognosis, and, importantly, inconsistency in their clinical management due to insufficient data NEC genetic features. In order widen our...
<h3>Introduction/Background</h3> Despite evidence suggesting a potential role for immunotherapy in Epithelial Ovarian Cancer (EOC), initial attempts had limited efficacy. A better characterization of tumor infiltrating lymphocytes' (TIL) immunophenotype appears crucial to deeply understand their anti-tumor immunity and set the basis modulation optimize adoptive cell therapies approaches. We extensively characterized composition phenotype immune cells EOC identify pathways involved limiting...