A5040771322- HIV Research and Treatment
- Extracellular vesicles in disease
- HIV-related health complications and treatments
- Nanoparticle-Based Drug Delivery
- Immune Cell Function and Interaction
- HIV/AIDS Research and Interventions
- Mosquito-borne diseases and control
- Cytomegalovirus and herpesvirus research
- Nanoplatforms for cancer theranostics
- HIV, Drug Use, Sexual Risk
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Neuroinflammation and Neurodegeneration Mechanisms
- Hydrogels: synthesis, properties, applications
- Immunotherapy and Immune Responses
- Circular RNAs in diseases
- Nanocluster Synthesis and Applications
- Viral-associated cancers and disorders
- Herpesvirus Infections and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Reproductive System and Pregnancy
- Alcohol Consumption and Health Effects
- IL-33, ST2, and ILC Pathways
- Tryptophan and brain disorders
- Autophagy in Disease and Therapy
Florida International University
2013-2025
Dr. Herbert & Nicole Wertheim Family Foundation
2023
University of Central Florida
2022
Morehouse School of Medicine
2010-2019
Texas Tech University
2017
Nanomed (Brazil)
2017
Institute of Neuroimmunology of the Slovak Academy of Sciences
2017
The Wistar Institute
2009-2012
National Hansen's Disease Program
2010
Louisiana State University
2010
Abstract Liquid biopsy can detect circulating cancer cells or tumor cell-derived DNA at various stages of cancer. The fluid from these biopsies contains extracellular vesicles (EVs), such as apoptotic bodies, microvesicles, exomeres, and exosomes. Exosomes contain proteins nucleic acids (DNA/RNA) that modify the microenvironment promote progression, playing significant roles in pathology. Clinically, within exosomes liquid be biomarkers for detection prognosis We review EVs protein miRNA...
HIV-1 Nef has been demonstrated to be integral for viral persistence, infectivity, and the acceleration of disease pathogenesis (AIDS) in humans. also detected plasma HIV-infected individuals is released from infected cells. The form which cells unknown. However, a myristoylated protein shown interact with intracellular vesicular trafficking network. Here we show that CD45-containing microvesicles. This microvesicular (mvNef) at relatively high concentrations (10 ng/ml). It present tissue...
The blood–brain barrier (BBB) is considered as the primary impediment for most drugs. Delivering therapeutic agents to brain still a big challenge date. In our study, dual mechanism, receptor mediation combined with external non-invasive magnetic force, was incorporated into ferrous magnet-based liposomes BBB transmigration enhancement. homogenous nanoparticles (MNPs), size of ∼10 nm, were synthesized and confirmed by TEM XRD respectively. classical magnetism assay showed presence...
Magneto-plasmonic nanoparticles are one of the emerging multi-functional materials in field nanomedicine. Their potential for targeting and multi-modal imaging is highly attractive. In this study, magnetic core/gold shell (MNP@Au) magneto-plasmonic were synthesized by citrate reduction Au ions on nanoparticle seeds. Hydrodynamic size optical properties with variation reducing agent concentrations evaluated. The exhibited superparamagnetic properties, their contributed to...
Magneto-plasmonic liposomes were developed for image-guided drug delivery, and showed multimodal imaging, enhanced BBB transmigration, reduction of viral replication.
Mechanisms that may allow circulating monocytes to persist as CD4 T cells diminish in HIV-1 infection have not been investigated. We characterized steady-state gene expression signatures from HIV-infected subjects and identified a stable antiapoptosis signature comprised of 38 genes associated with p53, CD40L, TNF, MAPK signaling networks. The significance this is indicated by our demonstration cadmium chloride- or Fas ligand-induced apoptosis resistance contrast increasing the same infected...
Human immunodeficiency virus (HIV) establishes latent infections in cellular reservoirs, including microglia. HC69 cells, a microglial model of HIV latency, contain an promoter long terminal repeat (LTR)-GFP reporter and were used for testing the efficacy two-step magnetoelectric nanoparticle (MENP) extracellular vesicle (xEV) latency-targeting (MELT) nanotherapeutic. GFP expression at rest is low (GFPLo), upon exposure to LTR, transcription-activating agents (i.e., TNF-α) are induced be...
New Findings What is the central question of this study? Does short‐term supplementation with l ‐citrulline in order to increase ‐arginine improve exercise blood flow and peripheral dilatation responses older adults? main finding its importance? ‐Citrulline increased femoral by 11% vascular conductance 14% during lower‐limb men, whereas no changes were observed women. This modest improvement bulk muscle men has implications for altering metabolism that may result enhanced tolerance adults....
Cocaine and other drugs of abuse increase HIV-induced immunopathogenesis; neurobiological mechanisms cocaine addiction implicate a key role for microRNAs (miRNAs), single-stranded non-coding RNAs that regulate gene expression defend against viruses. In fact, HIV defends miRNAs by actively suppressing the polycistronic miRNA cluster miRNA-17/92, which encodes including miR-20a. IFN-g production natural killer cells is regulated miR-155 this also critical to dendritic cell (DC) maturation....
The function of plasmacytoid dendritic cells (PDC) in chronic human immunodeficiency virus type 1 (HIV-1) infection remains controversial with regard to its potential for sustained alpha interferon (IFN-alpha) production and induction PDC-dependent tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL)-mediated cytotoxicity HIV-infected cells. We address these areas by a study chronically HIV-1-infected subjects followed through antiretroviral therapy (ART) interruption...
Although highly active antiretroviral therapy (HAART) has significantly reduced the morbidity and mortality in HIV patients, virus continues to reside central nervous system (CNS) reservoir. Hence, a complete eradication of remains challenge. productively infects microglia/macrophages, but astrocytes are generally restricted infection. The relative importance possible replication blocks astrocytes, however, is yet be delineated. A recently identified restriction factor, sterile alpha motif...
HIV-associated neurocognitive disorder (HAND) is characterized by development of cognitive, behavioral and motor abnormalities, occurs in approximately 50% HIV infected individuals. In the United States, prevalence cigarette smoking ranges from 35-70% HIV-infected individuals compared to 20% general population. Cognitive impairment heavy smokers has been well reported. However, synergistic effects nicotine infection underlying mechanisms HAND are unknown.In this study, we explored role...
The emerging field of theranostics for advanced healthcare has raised the demand effective and safe delivery systems consisting therapeutics diagnostics agents in a single monarchy. This requires development multi-functional bio-polymeric efficient image-guided therapeutics. study reports size-controlled (micro-to-nano) auto-fluorescent biopolymeric hydrogel particles chitosan hydroxyethyl cellulose (HEC) synthesized using water-in-oil emulsion polymerization technique. Sustainable resource...
Abstract Differential expression of metallothionein genes and high intracellular zinc promote monocyte survival during HIV infection. Circulating monocytes exhibit an apoptotic resistance phenotype viremia in association with increased MT expression. MTs are known to play important role metabolism immune function. We now show, a cross-sectional study using peripheral monocytes, that MT1 isoforms E, G, H, X is significantly circulating cells from HIV+ subjects chronic viremic episodes as...
Abstract: The human immunodeficiency virus 1 (HIV-1) still remains one of the leading life-threatening diseases in world. introduction highly active antiretroviral therapy has significantly reduced disease morbidity and mortality. However, most drugs have variable penetrance into viral reservoir sites, including gut-associated lymphoid tissue (GALT). Being largest organ, GALT plays a key role early HIV infection host–pathogen interaction. Many different treatment options been proposed to...
In present research work, for the first time, we demonstrate that neuropathogenesis in HIV-1 clade B and C infection is associated with IL-33 ST2 dysregulation, is, implication toward neuropathogenesis. It known of HIV infected individuals dependent. Proinflammatory cytokines related receptors play a significant role complex regulatory mechanisms infection. Among them, an inflammatory cytokine expressed central nervous system (CNS) activates microglia cells may affect neuroimmune processes...
Opiate abuse increases the risk of HIV transmission and exacerbates neuropathology by increasing inflammation modulating immune cell function. Exosomal EVs(xEV) contain miRNAs that may be differentially expressed due to infection or opiate abuse. Here we develop a preliminary exosomal-miRNA biomarker profile HIV-infected PBMCs in context use. infected with were treated dosages morphine for 72 hours, culture supernatants collected, exosomes isolated using differential centrifugation....
Abstract HIV subtypes or clades differentially induce HIV-associated neurocognitive disorders (HAND) and substance abuse is known to accelerate disease progression. The HIV-1 envelope protein gp120 plays a major role in binding budding the central nervous system (CNS) impacts dopaminergic functions. However, mechanisms utilized by exert differential effects methamphetamine (METH)-associated dysfunction are poorly understood. We hypothesized that clade B C structural sequences, modeling based...