Biofilm formation is recognized as the main virulence factor in a variety of chronic infections. In vitro evaluation biofilm often achieved by quantification viable or total cells. However, these methods depend on disruption, which vortexing sonication. this study, we investigated effects sonication elimination Staphylococcus epidermidis cell clusters from biofilms grown over time, and was performed three distinct analytical techniques. Even when higher number cycles used, some stable...

Staphylococcus epidermidis biofilm-related infections are a current concern within the medical community due to their high incidence and prevalence, particularly in patients with indwelling devices. Biofilm gene expression analysis by quantitative real-time PCR (qPCR) has been increasingly used understand role of biofilm formation pathogenesis S. infections. However, depending on RNA extraction procedure, cDNA synthesis qPCR master mixes used, quantification can be suboptimal. We recently...

The physiological function of Ataxin-3 (ATXN3), a deubiquitylase (DUB) involved in Machado–Joseph Disease (MJD), remains elusive. In this study, we demonstrate that ATXN3 is required for neuronal differentiation and normal cell morphology, cytoskeletal organization, proliferation survival SH-SY5Y PC12 cells. This cellular phenotype associated with increased proteasomal degradation α5 integrin subunit (ITGA5) reduced activation signalling rescued by ITGA5 overexpression. Interestingly,...

Aim: To understand the relationship between ica, aap and bhp gene expression implications in biofilm formation selected clinical commensal Staphylococcus epidermidis isolates. Material & methods: Isolates were analyzed regarding their biofilm-forming capacity, biochemical matrix composition, spatial organization of biofilm-related genes. Results: On polysaccharide intercellular adhesin-dependent biofilms, contributions for growth negligible, despite very high levels expression. In contrast,...

"Green" protein purification/immobilization processes based on low-cost, earth-abundant, and eco-friendly affinity matrices are highly desirable. Unmodified silica fit well these demands. Since histidine-rich silica-binding peptides frequently isolated in biopanning experiments, this work aimed at assessing the viability of using bare as an alternative matrix for His-tagged proteins. Adsorption desorption studies with a purified His6-tagged EGFP shown that binding to particles different size...

Introdução: A hipertensão arterial é uma doença complexa, multifatorial, controlada por fatores genéticos e ambientais.Objetivo: Avaliar a susceptibilidade genética no aparecimento de sua associação com os risco tradicionais na eclosão desta patologia.Material Métodos: Estudo caso-controlo 1712 indivíduos, idade média 51,0 ± 7,9 anos (860 hipertensos 852 controlos). Avaliaram-se tradicionais, bioquímicos as variantes genéticas: ACE I/D rs4340, A2350G rs4343, AGT T174M rs4762, M235T rs699...

Several genetic risk scores (GRS) have been associated with cardiovascular disease; their role, however, in survival from proven coronary artery disease (CAD) yielded conflicting results.The objective of this study was to evaluate long-term mortality according the score a Southern European population CAD.A cohort 1464 CAD patients angiographic were followed up prospectively for 58.3 (interquartile range: 25.8-88.1) months. Genotyping 32 single-nucleotide polymorphisms previously performed...

Background: Genetic risk score can quantify individual’s predisposition to coronary artery disease; however, its usefulness as an independent predictor remains inconclusive. Objective: To evaluate the incremental predictive value of a genetic traditional factors associated with disease. Methods: Thirty-three variants previously disease were analyzed in case-control population 2,888 individuals. A multiplicative was calculated and then divided into quartiles, 1st quartile reference class....

The utility of genetic risk scores (GRS) as independent predictors remains inconclusive. Here, we evaluate the additive value a multi-locus GRS to Framingham score (FRS) in coronary artery disease (CAD) prediction. A total 2888 individuals (1566 patients and 1322 controls) were divided into three subgroups according FRS. Multiplicative was determined for 32 variants associated CAD. Logistic Regression Area Under Curve (AUC) first, using TRF each FRS subgroup, secondly, adding GRS. Different...

Coronary artery disease (CAD), characterized by an atherogenic process in the coronary arteries, is one of leading causes death Madeira. The GENEMACOR (GENEs MAdeira and CORonary Disease) study sought to investigate main risk factors - environmental genetic estimate whether a score (GRS) improves CAD prediction, discrimination reclassification.Traditional 33 variants were considered case-control with 3139 individuals (1723 patients 1416 controls). multivariate analysis assessed likelihood...

Essential hypertension (EH) is a complex disease in which physiological, environmental, and genetic factors are involved its genesis. The variant of the alpha-adducin gene (ADD1) has been described as risk factor for EH, but with controversial results. objective this study was to evaluate association ADD1 (Gly460Trp) polymorphism EH population from Madeira Island. A case-control 1614 individuals Caucasian origin performed, including 817 797 controls. Cases controls were matched sex age, by...

Essential hypertension (EH) is a disease in which both environment and genes have an important role. This study was designed to identify the interaction model between genetic variants environmental risk factors that most highly potentiates EH development.We performed case-control with 1641 participants (mean age 50.6 ± 8.1 years), specifically 848 patients 793 controls, adjusted for gender age. Traditional factors, biochemical parameters, including genotypic discrimination of 14 previously...

A hipertensão arterial é um problema de Saúde Pública, afeta 25% da população adulta mundial. Fatores genéticos e ambientais contribuem para a sua patogénese. O polimorfismo C825T subunidade β3 Proteína G (rs5443) favorece produção uma variante alternativa, truncada, que facilita sinalização intracelular, pode interferir na regulação pressão arterial. Essa genética tem sido descrita como fator risco arterial, com resultados controversos. Avaliar associação do gene GNβ3 o aparecimento numa...

Healthcare-associated infections (HAIs) have been increasing during recent decades, leading to long hospital stays and high morbidity mortality rates. The usage of antibiotics therapy against these is enhancing the emergence more multiple-drug resistant strains, in particular Staphylococcus epidermidis. Hence, this study focused on resistance pattern S. epidermidis isolates from clinical settings its association with phenotypic molecular traits. Our results showed that HAIs were prevalent...

The long-term follow-up of very preterm and low birthweight cohorts contributes to valuable evidence understand life-course outcomes in these vulnerable populations. However, attrition is a major challenge outcome studies. Examining the techniques used by existing may help reveal practices that enhance willingness continue participation over time.To evaluate effect data collection methods retention strategies on overall European birth individuals born birthweight.A survey integrated...

To evaluate the synergistic activity of antimicrobial drugs against lineages methicillin-resistant Staphylococcus aureus (MRSA) carrying SCCmec IV. The biofilm production and related genes were also detected.Forty two MRSA isolates tested for genes. Biofilm/biomass susceptibility to gentamicin (G), linezolid (L), rifampicin (R) vancomycin (V) was determined six from three prevalent in Rio de Janeiro hospitals concentrations ranging 0·25 64 μg ml(-1). Biomass evaluated by microtitre plate...

Abstract Aim To assess the impact of late‐onset neonatal sepsis (LONS) on cognitive and motor development five‐year‐old children who were born very preterm (VPT). Methods This study included 327 VPT from Portuguese EPICE/SHIPS cohort attended neurodevelopment assessment. Neuropsychological tests such as WPPSI‐R, MABC‐2 NEPSY‐II (language domain) used to children's development. Statistical analysis was performed compare socio‐demographic, clinical outcomes with without LONS. Regression...

O Transtorno Dissociativo de Identidade (TDI) é uma condição complexa caracterizada pela fragmentação da identidade e dissociação consciência, frequentemente associada a traumas graves na infância. Este artigo revisa as principais abordagens diagnósticas para o TDI, explorando utilização do DSM-5 como referência clínica, em contraste com limitações CID-10. Instrumentos Escala Experiências Dissociativas (DES) técnicas projetivas, Rorschach, foram destacados por sua utilidade avaliação dos...

Frutalin is a plant lectin with beneficial immunobiological action, although the access to its active form still restricted. Moreover, there knowledge gap on isoform activity and glycosylation impact bioactivity, recombinant production protocols were seen as ineffective. Here, simpler faster purification protocol was developed, attaining yield of purified frutalin 3.3-fold higher than that obtained previously. Hemagglutination assays confirmed this could not agglutinate rabbit erythrocytes,...