A5051589662- Mesenchymal stem cell research
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Spinal Cord Injury Research
- Genetic Neurodegenerative Diseases
- Antimicrobial Resistance in Staphylococcus
- Essential Oils and Antimicrobial Activity
- Shoulder and Clavicle Injuries
- Pluripotent Stem Cells Research
- Erythrocyte Function and Pathophysiology
- Shoulder Injury and Treatment
- Cancer Cells and Metastasis
- Neurological and metabolic disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Diabetes and associated disorders
- 3D Printing in Biomedical Research
- Plant-Microbe Interactions and Immunity
- Antibiotics Pharmacokinetics and Efficacy
- Medicinal Plants and Neuroprotection
- Spinal Dysraphism and Malformations
- Muscle Physiology and Disorders
- Curcumin's Biomedical Applications
- Extracellular vesicles in disease
- Nuclear Structure and Function
- Streptococcal Infections and Treatments
University of Minho
2016-2025
University of Coimbra
2016
Universitat de les Illes Balears
1994
The use of human mesenchymal stem cells (hMSCs) has emerged as a possible therapeutic strategy for CNS-related conditions. Research in the last decade strongly suggests that MSC-mediated benefits are closely related with their secretome. Studies published recent years have shown secretome hMSCs isolated from different tissue sources may present significant variation. With this mind, work performed comparative proteomic-based analysis through mass spectrometry on derived bone marrow (BMSCs),...
AbstractQuorum sensing (QS) is an important regulatory mechanism in biofilm formation and differentiation. Interference with QS can affect development antimicrobial susceptibility. This study evaluates the potential of selected phytochemical products to inhibit QS. Three isothiocyanates (allylisothiocyanate – AITC, benzylisothiocyanate BITC 2-phenylethylisothiocyanate PEITC) six phenolic (gallic acid GA, ferulic FA, caffeic CA, phloridzin PHL, (−) epicatechin EPI oleuropein glucoside OG)...
In recent years it has been shown that the therapeutic benefits of human mesenchymal stem/stromal cells (hMSCs) in Central Nervous System (CNS) are mainly attributed to their secretome. The implementation computer-controlled suspension bioreactors be a viable route for expansion these large numbers. As hMSCs actively respond culture environment, there is hypothesis one can modulate its secretome through use. Herein, we present data indicating use enhanced neuroregulatory profile Indeed,...
Abstract Mesenchymal stem cells (MSCs) have been used for cell-based therapies in regenerative medicine, with increasing importance central and peripheral nervous system repair. However, MSCs grafting present disadvantages, such as, a high number of required transplantation low survival rate when transplanted into the (CNS). In line this, secretome which on its composition wide range molecules (neurotrophins, cytokines) microvesicles, can be solution to surpass these problems. effect axonal...
Massive rotator cuff tears (MRCTs) are usually chronic lesions with pronounced degenerative changes, where advanced fatty degeneration and atrophy can make the tear irreparable. Human mesenchymal stem cells (hMSCs) secrete a range of growth factors vesicular systems, known as secretome, that mediates regenerative processes in tissues undergoing degeneration.To study effect hMSC secretome on muscular changes shoulder function rat MRCT model.Controlled laboratory study.A bilateral 2-tendon...
Natural biomaterials have attracted an increasing interest in the field of tissue-engineered nerve grafts, representing a possible alternative to autologous transplantation. With prospect developing novel entubulation strategy for transected nerves with cell-seeded chitosan films, we examined biocompatibility such films vitro. Different types rat Schwann cells (SCs)--immortalized, neonatal, and adult-of substrate. Both cell were viable on biomaterial showed different metabolic activities...
Glioblastoma (GBM), the most malignant primary brain tumor, leads to poor and unpredictable clinical outcomes. Recent studies showed tumor microenvironment has a critical role in regulating growth by establishing complex network of interactions with cells. In this context, we investigated how GBM cells modulate resident glial cells, particularly their paracrine activity, modulation can influence back on phenotype Conditioned media (CM) mouse cultures unexposed (unprimed) or exposed (primed)...
The use of resistance-modifying agents is a potential strategy that used to prolong the effective life antibiotics in face increasing antibiotic resistance. Since certain flavonoids are potent bacterial efflux pump inhibitors, we assessed morin, rutin, quercetin, hesperidin, and (+)-catechin for their combined activity with ciprofloxacin, tetracycline, erythromycin, oxacillin, ampicillin against drug-resistant strains Staphylococcus aureus, including methicillin-resistant S. aureus. Four...
Priming strategies that improve the functionality of MSCs may be required to address issues limiting successful clinical translation MSC therapies. For conditions requiring high trophic support such as brain and spinal cord injuries, priming produce higher levels factors instrumental facilitate current We developed tested a novel molecular paradigm using docosahexaenoic acid (DHA) prime adipose tissue-derived mesenchymal stromal cells (ASCs) enhance secretome neuroregulatory potential.
Innovations in spinal cord injury (SCI) models are crucial for developing effective therapies. This study introduces a novel vitro SCI model using cultures of primary mixed cells from rat pups, featuring key cell types. offers distinct advantages terms feasibility, reproducibility, and cost-effectiveness, requiring only basic culture equipment. Following hyperosmotic stress via sorbitol treatment, the recapitulated pathophysiological hallmarks, with 65% reduction viability gradual death over...
The physiological function of Ataxin-3 (ATXN3), a deubiquitylase (DUB) involved in Machado–Joseph Disease (MJD), remains elusive. In this study, we demonstrate that ATXN3 is required for neuronal differentiation and normal cell morphology, cytoskeletal organization, proliferation survival SH-SY5Y PC12 cells. This cellular phenotype associated with increased proteasomal degradation α5 integrin subunit (ITGA5) reduced activation signalling rescued by ITGA5 overexpression. Interestingly,...
Parkinson’s disease (PD) is the most common movement disorder, characterized by progressive loss of dopaminergic neurons from nigrostriatal system. Currently, there no treatment that retards progression or reverses damage prior to time clinical diagnosis. Mesenchymal stem cells (MSCs) are one extensively studied cell sources for regenerative medicine applications, particularly due release soluble factors and vesicles, known as secretome. The main goal this work was address therapeutic...
Introduction The inflammatory response after spinal cord injury (SCI) is an important contributor to secondary damage. Infiltrating macrophages can acquire a spectrum of activation states, however, the microenvironment at SCI site favors macrophage polarization into pro-inflammatory phenotype, which one reasons why transplantation has failed. Methods In this study, we investigated therapeutic potential secretome for recovery. We effect in vitro using peripheral and CNS-derived neurons human...
The low regeneration potential of the central nervous system (CNS) represents a challenge for development new therapeutic strategies neurodegenerative diseases, including spinocerebellar ataxias. Spinocerebellar ataxia type 3 (SCA3)-or Machado-Joseph disease (MJD)-is most common dominant ataxia, being mainly characterized by motor deficits; however, SCA3/MJD has complex and heterogeneous pathophysiology, involving many CNS brain regions, contributing to lack effective therapies. Mesenchymal...
Abstract: Y-27632 is a well-known inhibitor of the Rho-associated coiled kinase (ROCK) and has been shown to significantly improve culture variety multipotent stem cell types. However, effects on expansion adult human adipose-derived (hADSC) cultures remain be established. Here, we aimed characterize hADSCs. Adult hADSCs were isolated from subjects submitted elective plastic surgery procedures cultivated in vitro under optimized conditions. Our results show that continuous supplementation...
Objective: The objective of this study was to evaluate the level understanding and adherence CHW according protocol Itaberaí Project (PI), aiming at breast cancer screening. Methodology: This is a clinical, controlled, randomized, multicenter, phase III trial, where unit observation engagement community health workers (CHW) in PI. With randomization, were randomly allocated control group (CG) intervention (IG). For evaluation comparison between groups levels Project, specific questionnaire...