The use of human mesenchymal stem cells (hMSCs) has emerged as a possible therapeutic strategy for CNS-related conditions. Research in the last decade strongly suggests that MSC-mediated benefits are closely related with their secretome. Studies published recent years have shown secretome hMSCs isolated from different tissue sources may present significant variation. With this mind, work performed comparative proteomic-based analysis through mass spectrometry on derived bone marrow (BMSCs),...

Abstract Research in the last decade strongly suggests that mesenchymal stem cell (MSC)-mediated therapeutic benefits are mainly due to their secretome, which has been proposed as a possible tool for treatment of Parkinson's disease (PD). Indeed, it shown MSC secretome increases neurogenesis and survival, numerous neuroprotective actions under different conditions. Additionally, using dynamic culturing conditions (through computer-controlled bioreactors) can further modulate thereby...

Abstract Intercellular communication is vital to ensure tissue and organism homeostasis can occur directly, between neighbour cells via gap junctions (GJ), or indirectly, at longer distances, through extracellular vesicles, including exosomes. Exosomes, as intercellular carriers of messenger molecules, mediate the transfer biological information donor acceptor cells. Although effects exosomes in target have been intensively studied, mechanisms that govern exosomal uptake are not fully...

In recent years it has been shown that the therapeutic benefits of human mesenchymal stem/stromal cells (hMSCs) in Central Nervous System (CNS) are mainly attributed to their secretome. The implementation computer-controlled suspension bioreactors be a viable route for expansion these large numbers. As hMSCs actively respond culture environment, there is hypothesis one can modulate its secretome through use. Herein, we present data indicating use enhanced neuroregulatory profile Indeed,...

Parkinson's disease (PD) is characterized by a selective loss of dopamine (DA) neurons in the human midbrain causing motor dysfunctions. The exact mechanism behind dopaminergic cell death still not completely understood and, so far, no cure or neuroprotective treatment for PD available. Recent studies have brought attention to variety bioactive molecules produced mesenchymal stem cells (MSCs), generally referred as secretome. Herein, we evaluated whether MSCs-bone marrow derived (hBMSCs)...

The quantification of large proteomes across multiple samples has become the major focus proteomics. In addition to advantages in-gel digestion, extensive time and sample handling required have precluded use this type method for quantitative screens. Therefore, an adaptation digestion method, termed short-GeLC, is proposed as a faster more reproducible preparation approaches. methodology was compared with two well-established procedures preparation, GeLC-MS classic liquid followed by LC-MS,...

The mitochondrion is emerging as a key organelle in stem cell biology, acting regulator of pluripotency and differentiation. In this study we sought to understand the effect mitochondrial complex III inhibition during neuronal differentiation mouse embryonic cells. When exposed antimycin A, specific inhibitor, cells failed differentiate into dopaminergic neurons, maintaining high Oct4 levels even when subjected protocol. Mitochondrial affected distinct populations present culture, inducing...

The use of human umbilical cord Wharton Jelly-derived mesenchymal stem cells (hWJ-MSCs) has been considered a new potential source for future safe applications in regenerative medicine. Indeed, the application hWJ-MSCs into different animal models disease, including those from central nervous system, shown remarkable therapeutic benefits mostly associated with their secretome. Conventionally, are cultured and characterized under normoxic conditions (21 % oxygen tension), although levels...

Massive rotator cuff tears (MRCTs) are usually chronic lesions with pronounced degenerative changes, where advanced fatty degeneration and atrophy can make the tear irreparable. Human mesenchymal stem cells (hMSCs) secrete a range of growth factors vesicular systems, known as secretome, that mediates regenerative processes in tissues undergoing degeneration.To study effect hMSC secretome on muscular changes shoulder function rat MRCT model.Controlled laboratory study.A bilateral 2-tendon...

Abstract Parkinson's disease (PD) is a progressive neurodegenerative movement disorder that results from the death of dopamine (DA) neurons. Over recent years, differentiated or undifferentiated neural stem cells (NSCs) transplantation has been widely used as means cell replacement therapy. However, compelling evidence brought attention to array bioactive molecules produced by cells, defined secretome. As described in literature, other populations have high-neurotrophic activity, but little...

The coordinated and synchronized cardiac muscle contraction relies on an efficient gap junction-mediated intercellular communication (GJIC) between cardiomyocytes, which involves the rapid anisotropic impulse propagation through connexin (Cx)-containing channels, namely of Cx43, most abundant Cx in heart. Expectedly, disturbing mechanisms that affect channel activity, localization turnover Cx43 have been implicated several cardiomyopathies, such as myocardial ischemia. Besides communication,...

Patients suffering from spinal cord injury (SCI) still have a dismal prognosis. Despite all the efforts developed in this area, currently there are no effective treatments. Therefore, cell therapies been proposed as viable alternative to current treatments used. Adipose tissue-derived stromal cells (ASCs) and olfactory ensheathing (OECs) used with promising results different models of SCI, namely due regenerative properties secretome first, guidance capability second. Using an vitro model...

Proteomic approaches are extremely valuable in many fields of research, where mass spectrometry methods have gained an increasing interest, especially because the ability to perform quantitative analysis. Nonetheless, sample preparation prior analysis is utmost importance. In this work, two protein precipitation approaches, widely used for cleaning and concentrating samples, were tested compared very diluted samples solubilized a strong buffer (containing SDS). The amount recovered after...

Oxidation–reduction post-translational modifications (redox-PTMs) are chemical alterations to amino acids of proteins. Redox-PTMs participate in the regulation protein conformation, localization and function, acting as signalling effectors that impact many essential biochemical processes cells. Crucially, dysregulation redox-PTMs proteins has been implicated pathophysiology numerous human diseases, including neurodegenerative diseases such Alzheimer’s disease Parkinson’s disease. This review...

Glioblastoma (GBM), the most malignant primary brain tumor, leads to poor and unpredictable clinical outcomes. Recent studies showed tumor microenvironment has a critical role in regulating growth by establishing complex network of interactions with cells. In this context, we investigated how GBM cells modulate resident glial cells, particularly their paracrine activity, modulation can influence back on phenotype Conditioned media (CM) mouse cultures unexposed (unprimed) or exposed (primed)...

Glioblastoma (GBM) is a highly aggressive primary brain cancer, for which curative therapies are not available. An emerging therapeutic approach suggested to have potential target malignant gliomas has been based on the use of multipotent mesenchymal stem cells (MSCs), either unmodified or engineered deliver anticancer agents, as these present an intrinsic capacity migrate towards tumors. Nevertheless, it still controversial whether this innate tropism MSCs tumor area associated with cancer...

Mitochondrial deregulation has gained increasing support as a pathological mechanism in Huntington's disease (HD), genetic-based neurodegenerative disorder caused by CAG expansion the HTT gene. In this study, we thoroughly investigated mitochondrial-based mechanisms HD patient-derived iPSC (HD-iPSC) and differentiated neural stem cells (NSC) versus control cells, well subjected to CRISPR/Cas9-CAG repeat deletion. We analyzed mitochondrial morphology, function biogenesis, linked exosomal...

Abstract The pinewood nematode, Bursaphelenchus xylophilus , recognized as a worldwide major forest pest, is migratory endoparasitic nematode with capacity to feed on pine tissues and also fungi colonizing the trees. mucronatus closest related species, differs from B. its pathogenicity, making this good candidate for comparative analyses. Secretome profiles of were obtained proteomic differences evaluated by quantitative SWATH-MS. From 681 proteins initially identified, 422 quantified...

Abstract Mitochondrial and autophagy dysfunction are mechanisms proposed to be involved in the pathogenesis of several neurodegenerative diseases. Huntington's disease (HD) is a progressive disorder associated with mutant Huntingtin‐induced abnormalities neuronal mitochondrial dynamics quality control. Former studies suggest that removal defective mitochondria may compromised HD. control (MQC) complex, well‐orchestrated pathway can through mitophagy dysregulation or impairment...

Abstract In echinoderms, the coelomic epithelium (CE) is reportedly source of new circulating cells (coelomocytes) as well provider molecular factors such immunity-related molecules. However, its overall functions have been scarcely studied in detail. this work, we used an integrated approach based on both microscopy (light and electron) proteomic analyses to investigate arm CE starfish Marthasterias glacialis during different physiological conditions (i.e., non-regenerating and/or...