A5070210143- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Nanopore and Nanochannel Transport Studies
- Nanoplatforms for cancer theranostics
- RNA and protein synthesis mechanisms
- Viral Infections and Immunology Research
- Molecular Communication and Nanonetworks
- Spider Taxonomy and Behavior Studies
- Nanocluster Synthesis and Applications
- Silk-based biomaterials and applications
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- Proteoglycans and glycosaminoglycans research
- Electrochemical Analysis and Applications
- Advanced Biosensing Techniques and Applications
- Parvovirus B19 Infection Studies
- SARS-CoV-2 detection and testing
- Infective Endocarditis Diagnosis and Management
Chongqing Medical University
2022-2025
Wuhan University
1994-2024
University of Science and Technology of China
2018-2024
Zhongnan Hospital of Wuhan University
2024
Hunan Normal University
2018-2023
Dalian Medical University
2022
Second Affiliated Hospital of Chongqing Medical University
2022
BGI Group (China)
2017-2021
Xijing Hospital
2016-2021
University of Chinese Academy of Sciences
2020-2021
Abstract In nature, the formation of spider silk fibers begins with dimerizing pH‐sensitive N‐terminal domains proteins (spidroins) upon lowering pH, and provides a natural masterpiece for programmable assembly. Inspired by similarity pH‐dependent dimerization behaviors, introduced here is an i‐motif‐guided model to mimic initial step spidroin assembly at subcellular level. A framework nucleic acid (FNA) nanoplatform designed using two tetrahedral DNA nanostructures (TDNs) different branched...
Lions are one of the world’s most iconic megafauna, yet little is known about their temporal and spatial demographic history population differentiation. We analyzed a genomic dataset 20 specimens: two ca. 30,000-y-old cave lions ( Panthera leo spelaea ), 12 historic leo/Panthera melanochaita ) that lived between 15th 20th centuries outside current geographic distribution lions, 6 present-day from Africa India. found modern shared an ancestor 500,000 y ago 2 lineages likely did not hybridize...
Abstract In nature, intact apoptotic cells release ATP as a signaling molecule to trigger prompt phagocytic clearance, even at the earliest stage of apoptosis. Inspired by this, here we introduce straightforward strategy for real‐time monitoring exocytosis and drug‐stimulated apoptosis in cancer cell surroundings. Triplex‐boosted G‐quadruplexes (tb‐G4s) responding environmental factors (H + K ) are engineered construct DNA logic‐gated nanoplatform proximity aptasensing on surface. It enables...
Geckos are among the most species-rich reptile groups and sister clade to all other lizards snakes. possess a suite of distinctive characteristics, including adhesive digits, nocturnal activity, hard, calcareous eggshells, lack eyelids. However, one gecko clade, Eublepharidae, appears be exception these 'rules' lacks toe pads, has eyelids, lays eggs with soft, leathery eggshells. These differences make eublepharids an important component any investigation into underlying genomic innovations...
DNAzymes have emerged as an important class of sensors for a wide variety metal ions, with florescence DNAzyme the most widely used in different sensing and imaging applications because their fast response time, high signal intensity, sensitivity. However, requirements external excitation light source its associated power increase cost size fluorometer, making it difficult to be portable detections. To overcome these limitations, we report herein sensor that relies on chemiluminescence...
Abstract Background Understanding the rate and pattern of germline mutations is fundamental importance for understanding evolutionary processes. Results Here we analyzed 19 parent-offspring trios rhesus macaques (Macaca mulatta) at high sequencing coverage ∼76× per individual estimated a mean 0.77 × 10−8de novo site generation (95% CI: 0.69 10−8 to 0.85 10−8). By phasing 50% parental origins, found that mutation positively correlated with paternal age. The lineage contributed 81% de...
We sequenced the genome of strawberry poison frog, Oophaga pumilio, at a depth 127.5× using variable insert size libraries. The total is estimated to be 6.76 Gb, which 4.76 Gb are from high copy number repetitive elements with low differentiation across copies. These repeats encompass DNA transposons, RNA and LTR retrotransposons, including least 0.4 1.0 Mariner/Tc1 Gypsy elements, respectively. Expression data indicate levels gypsy expression in ova O. pumilio compared Xenopus laevis....
Abstract Developing synergistic targeted therapeutics to improve treatment efficacy while reducing side effects has proven promising for anticancer therapies, but how conveniently modulate multidrug cooperation remains a challenge. Here, novel strategy using G‐quadruplex‐programmed versatile nanorobot (G4VN) containing two subunits of DNAzyme (DzG4) and ligand‐drug conjugates (LDCs) is proposed precisely target tumors then execute both gene silencing chemotherapy. As the core module this...
An enzyme-free stochastic DNA walker propelled by a single catalytic or double assembly has been constructed.
A rational DNA nanomachine was constructed for HIV nucleic acid ultrasensitive sensing.
Acute myocardial infarction (AMI)-related microRNAs (miRNAs) in circulating blood have been proved as promising biomarkers for AMI diagnosis. The detection of these miRNAs at ultralow levels usually requires nucleic acid amplification strategies to improve the sensitivity cost time. Given that first hour after an attack is golden time saving patients' lives, shortening ultrasensitive miRNA analysis great significance clinical Toward this goal, here we present a direct...
Detection of viruses with high sensitivity is critical for the prevention and treatment related disease. Two homogeneous target-induced cascade amplification methods were proposed detection enterovirus 71 coxsackievirus B3. These both employ DNAzyme but differ in way which amplified. In hybridization chain reaction (HCR)-based strategy, assembled by hairpin DNA strands, while rolling circle (RCA)-based synthesized polymerase. On basis virion structure, we investigated effects using only...
As a common hairpin-based amplification strategy, catalytic-hairpin assembly (CHA) has been widely used to construct various DNA circuits for biosensing and imaging. However, the hairpin substrates can potentially react without catalysts result in circuit leakage, which may be quite severe CHA reaction consisting of three or four hairpins due formation stable three-/four-way junction product. To circumvent this problem, here we introduce well-designed ATP aptamer as brake into four-hairpin...
When dealing with infectious pathogens, the risk of contamination or infection in process detecting them is nonnegligible. Separation-free detection will be beneficial operation and safety. In this work, we proposed a DNAzyme walker for homogeneous isothermal enterovirus. The divided into two inactivate subunits. subunit-conjugated antibody binds to target virus, activity recovers as result spatial proximity. propels, fluorescence recovers. final intensity reaction mixture related...
The relatively low stability and polymorphism of bimolecular G-quadruplexes (bi-G4s) are big difficulties that faced in employing them to guide DNA assembly, as they usually subject a transformation into more stable tetramolecular or G-wire structures favored by K+ Mg2+. Although bi-G4s benefit additional duplex handles, challenge remains tailoring their intrinsic properties resolve the above difficulties. Toward this challenge, here we engineer several ultrastable via replacing nucleotide...
Abstract I-motif DNAs have been widely employed as robust modulating components to construct reconfigurable DNA nanodevices that function well in acidic cellular environments. However, they generally display poor interactivity with fluorescent ligands under these complex conditions, illustrating a major difficulty utilizing i-motifs the light-up system for label-free nanoassemblies and bioimaging. Towards addressing this challenge, here we devise new types of i-motif/miniduplex hybrid...
Abstract In nature, the formation of spider silk fibers begins with dimerizing pH‐sensitive N‐terminal domains proteins (spidroins) upon lowering pH, and provides a natural masterpiece for programmable assembly. Inspired by similarity pH‐dependent dimerization behaviors, introduced here is an i‐motif‐guided model to mimic initial step spidroin assembly at subcellular level. A framework nucleic acid (FNA) nanoplatform designed using two tetrahedral DNA nanostructures (TDNs) different branched...
Here, fluorescent molecular rotors are employed to develop a new type of high-performance FRET system with large Stokes shift, high photostability, and pH insensitivity, showing great promise for use in proximity-dependent DNA aptasensors. Two carboxylated benzothiazole-based synthesized, displaying bright green red fluorescence once labeled DNA. In the proximity state, an efficient occurs between two dyes, comparable that most commonly used Cy3/Cy5 pair. Similar phenomena also observed if...