A5039742206- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Plant responses to water stress
- Sugarcane Cultivation and Processing
- Plant Genetic and Mutation Studies
- Plant Stress Responses and Tolerance
- Cancer-related molecular mechanisms research
- Congenital heart defects research
- Rice Cultivation and Yield Improvement
- Heat shock proteins research
- Transgenic Plants and Applications
- Plant-Microbe Interactions and Immunity
- Genetic and Environmental Crop Studies
- Plant Reproductive Biology
- Bacteriophages and microbial interactions
- Photosynthetic Processes and Mechanisms
- Cellular Mechanics and Interactions
- Agriculture, Land Use, Rural Development
- Cardiomyopathy and Myosin Studies
- Plant Parasitism and Resistance
- Lipid metabolism and disorders
- Ubiquitin and proteasome pathways
- Plant biochemistry and biosynthesis
- Insect and Pesticide Research
Justus-Liebig-Universität Gießen
2020-2025
RWTH Aachen University
2017-2021
Sugarcane Breeding Institute
2014-2016
Max Planck Institute for Heart and Lung Research
2016
Indian Council of Agricultural Research
2014-2015
Plants offer fast, flexible and easily scalable alternative platforms for the production of pharmaceutical proteins, but differences between plant mammalian N-linked glycans, including presence β-1,2-xylose core α-1,3-fucose residues in plants, can affect activity, potency immunogenicity plant-derived proteins. Nicotiana benthamiana is widely used transient expression recombinant proteins so it desirable to modify endogenous N-glycosylation machinery allow synthesis complex N-glycans lacking...
During cardiac trabeculation, cardiomyocytes delaminate from the outermost (compact) layer to form complex muscular structures known as trabeculae. As these delaminate, remodeling of adhesion junctions must be tightly coordinated so cells can extrude compact while remaining in tight contact with their neighbors. In this study, we examined distribution N-cadherin (Cdh2) during trabeculation zebrafish. By analyzing localization a Cdh2-EGFP fusion protein expressed under control zebrafish cdh2...
Plant growth during abiotic stress is a long sought-after trait especially in crop plants the context of global warming and climate change. Previous studies on leaf epidermal cells have revealed that normal development, adjacent interdigitate anisotropically to form cell morphological patterns known as interlocking marginal lobes (IMLs), involving wall–cell membrane–cortical actin continuum. IMLs are growth-associated changes which auxin-binding protein (ABP), Rho GTPases play important...
<title>Abstract</title> Background Faba bean (<italic>Vicia faba</italic> L.) is an important legume that enhances agricultural sustainability through nitrogen fixation and possesses high nutritional value. Breeding of faba laborious time consuming, trait improvement could potentially be accelerated by new breeding technologies including gene editing, however tissue culture genetic transformation are also challenging in <italic>V. faba</italic>. Results We established a universal protocol...
Understanding sorghum response to water stress at different developmental stages is important for developing varieties with improved tolerance drought. This study set out finely characterize key agro-physiological responses and use of subjected drought scenarios. A greenhouse experiment was conducted using a DroughtSpotter facility that enables real-time quantification by gravimetric tracking. Four treatments were assessed: (i) early vegetative (DS1), (ii) DS1 plus recovery (DS1R), (iii)...
Abstract DNA‐free genome editing involves the direct introduction of ribonucleoprotein (RNP) complexes into cells, but this strategy has rarely been successful in plants. In present study, we describe a new technique for RNPs plant cells involving generation cavitation bubbles using pulsed laser. The resulting shockwave achieves efficient transfection walled tissue explants by creating transient membrane pores. RNP‐containing were rapidly identified fluorescence microscopy, followed...
Since the discovery of tobacco mosaic virus in 1890s, awareness has grown regard to how viruses affect environment. Viral infections are now known cause various effects besides pathogenicity, with some fact having a beneficial impact on plants. Although research focused disease-causing that can infect plants, many wild plants also infected non-pathogenic viral agents. Traditionally, abiotic, and biotic stresses have been studied as isolated stimuli trigger signaling pathways within plant....
Genome editing technologies possess significant potential to enhance plant breeding; however, the delivery of constructs poses challenges in numerous crop species due their resistance transformation or tissue culture. This study introduces an innovative approach for ribonucleoprotein (RNP) complexes and plasmid vectors into intact, regenerable faba bean tissues. is accomplished by applying electric current that makes cell walls membranes permeable, thereby allowing entry macromolecular...
Abstract DNA-free genome editing involves the direct introduction of ribonucleoprotein (RNP) complexes into cells, but this strategy has rarely been successful in plants. Here we describe a new technique for RNPs plant cells involving generation cavitation bubbles using pulsed laser. The resulting shockwave achieves efficient transfection walled tissue explants by creation transient membrane pores. RNP-containing were rapidly identified fluorescence microscopy, followed regeneration and...
Abstract DNA-free genome editing involves the direct introduction of ribonucleoprotein (RNP) complexes into cells, but this strategy has rarely been successful in plants. In present study, we describe a new technique for RNPs plant cells involving generation cavitation bubbles using pulsed laser. The resulting shockwave achieves efficient transfection walled tissue explants by creating transient membrane pores. RNP-containing were rapidly identified fluorescence microscopy, followed...