A5041122063- Cellular Mechanics and Interactions
- Axon Guidance and Neuronal Signaling
- Neuroscience and Neural Engineering
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Force Microscopy Techniques and Applications
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Lipid Membrane Structure and Behavior
- Neurogenesis and neuroplasticity mechanisms
- Micro and Nano Robotics
- Signaling Pathways in Disease
- Molecular Communication and Nanonetworks
- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Cell Adhesion Molecules Research
Nara Institute of Science and Technology
2015-2024
The growth cone, a motile structure located at the tip of growing axons, senses extracellular guidance cues and translates them into directional forces that drive axon outgrowth guidance. Axon directed by chemical on adhesive substrate is termed haptotaxis. Recent studies reported netrin-1 functions as haptotactic cue. However, mechanism mediating netrin-1–induced axonal haptotaxis remains unclear. Here, we demonstrate substrate-bound induces facilitating physical interactions between...
Motile cells transduce environmental chemical signals into mechanical forces to achieve properly controlled migration. This signal–force transduction is thought require regulated coupling between actin filaments (F-actins), which undergo retrograde flow at the cellular leading edge, and cell adhesions via linker “clutch” molecules. However, molecular machinery mediating this regulatory remains unclear. Here we show that F-actin binding molecule cortactin directly interacts with a clutch...
Highlights•Shootin1b accumulates at the leading process growth cone of olfactory interneurons•Shootin1b couples F-actin retrograde flow and cell adhesions as a clutch molecule•The shootin1b-mediated coupling generates force for neuronal migration•Loss shootin1 inhibits rostral migration interneurons in brainSummaryAs an essential step brain morphogenesis, neurons migrate via mechanical interactions with components their environment such neighboring cells extracellular matrix. However,...
Dendritic spines constitute the major compartments of excitatory post-synapses. They undergo activity-dependent enlargement, which is thought to increase synaptic efficacy underlying learning and memory. The spine enlargement requires activation signaling pathways leading promotion actin polymerization within spines. However, molecular machinery that suffices for structural plasticity remains unclear. Here, we demonstrate shootin1a links polymerizing filaments in with cell-adhesion molecules...
To establish functional networks, neurons must migrate to their appropriate destinations and then extend axons toward target cells. These processes depend on the advances of growth cones that located at tips neurites. Axonal generate driving forces by sensing local microenvironment modulating cytoskeletal dynamics actin-adhesion coupling (clutch coupling). Decades research have led identification guidance molecules, receptors, downstream signaling cascades for regulating neuronal migration...
SUMMARY Actin dynamics mediate cell morphogenesis. filaments polymerize outward at protrusions such as the leading edge of migrating cells, thereby pushing membrane to protrude. The current paradigm explains that actin are regulated by signaling. However, it is unclear how cells spontaneously form actin-based even without a specific local signaling cue. We found arrays treadmilling emerge widely in and move direction polymerization waves. Their arrival periphery pushes plasma Furthermore,...
To establish functional networks, neurons must migrate to their appropriate destinations and then extend axons toward target cells. These processes depend on the advances of growth cones that located at tips neurites. Axonal generate driving forces by sensing local microenvironment modulating cytoskeletal dynamics actin-adhesion coupling (clutch coupling). Decades research have led identification guidance molecules, receptors, downstream signaling cascades for regulating neuronal migration...
Dendritic spine enlargement by synaptic activation is thought to increase efficacy underlying learning and memory. This process requires forces generated actin polymerization actin-adhesion coupling (clutch coupling). Here, we describe a protocol monitor filament retrograde flow within spines using standard epi-fluorescence microscope. In combination with chemical long-term potentiation, this allows us quantify clutch efficiency rate, which are essential variables for generating...
SUMMARY Neurons migrate in a saltatory manner by repeating two distinct steps: extension of the leading process and translocation cell body. The former step is critical for determining migratory route response to extracellular guidance cues. In latter step, neurons must generate robust forces that translocate bulky soma against mechanical barriers surrounding three-dimensional environment. However, link between subsequent somal remains unknown. By using scanning ion conductance microscopy,...
Abstract The growth cone, a motile structure located at the tip of growing axon, senses extracellular guidance cues and translates them into directional forces that drive axon outgrowth guidance. Axon directed by chemical on adhesive substrate is termed haptotaxis. Recent studies reported netrin-1 functions as haptotactic cue. However, mechanism mediating netrin-1–induced axonal haptotaxis remains unclear. Here, we demonstrate substrate-bound induces facilitating physical interactions...