A5074564676- Digital Holography and Microscopy
- Optical measurement and interference techniques
- Advanced X-ray Imaging Techniques
- Microfluidic and Bio-sensing Technologies
- Blood properties and coagulation
- Sperm and Testicular Function
- Orbital Angular Momentum in Optics
- Digital Imaging for Blood Diseases
- Optical Imaging and Spectroscopy Techniques
- Electrohydrodynamics and Fluid Dynamics
- Image Processing Techniques and Applications
- Reproductive Biology and Fertility
- Spectroscopy Techniques in Biomedical and Chemical Research
- Corneal surgery and disorders
- Photoacoustic and Ultrasonic Imaging
Anhui Medical University
2022-2024
Point-of-care testing (POCT) plays an increasingly important role in biomedical research and health care. Quantitative phase microscopes (QPMs) with good contrast, no invasion, labeling, high speed automation could be effectively applied for POCT. However, most QPMs are fixed on the optical platform bulky size, lack of timeliness, which remained challenging POCT solutions. In this paper, we proposed a plug-and-play QPM multimode imaging based quantitative differential contrast (qDPC) method....
Classification of the morphology red blood cells (RBCs) plays an extremely important role in evaluating quality long-term stored blood, as RBC storage lesions such transformation discocytes to echinocytes and then spherocytes may cause adverse clinical effects. Most segmentation classification methods, limited by interference staining procedures poor details, are based on traditional bright field microscopy. In present study, quantitative phase imaging (QPI) technology was combined with deep...
It is important to measure the deformability of red blood cells (RBCs) before transfusion, which a key factor in gas transport ability RBCs and changes during storage vitro. Moreover, morphology also storage. proposed that change related deformability. However, efficiency typical methods use particles as handles low, especially measurement echinocyte spherocytes. Therefore, with different morphologies hard be measured compared same experiment. In this study, we developed cost-effective...
Quantitative differential phase contrast (qDPC) microscopy can achieve imaging of unlabeled cell samples with high spatial resolution. However, qDPC is easily affected by the noise generated in experiments. In this manuscript, L0-norm regularization was introduced into reconstruction for sparse (L0-qDPC), including quantitative target and samples. After detailed comparison fidelity, contrast, resolution, convergence rate, L0-qDPC method provide a stable without parameter adjustment due to...
The current classical blood smear technique to observe the morphology of single red cells (RBCs) for classification is a laborious and error-prone process. To objectively evaluate cells, we established method computational imaging based on programmable light emitting diode array. By using quantitative differential phase contrast (qDPC), characterized unlabeled RBCs as well smears. focusing comparing difference between stained under multimode microscopic technology, demonstrated that qDPC...
Distributive shock is considered to be a condition of microvascular hypoperfusion, which can fatal in severe cases. However, traditional therapeutic methods restore the macro blood flow are difficult accurately control perfusion microvessels, and currently developed manipulation techniques inevitably incompatible with biological systems. In our approach, infrared optical tweezers used dynamically reperfusion within subdermal capillaries pinna mice. Furthermore, we estimate effect different...
Motility is one of the most critical features to evaluate sperm quality. As longitudinal rolling human has long been ignored until recently, its detailed dynamics and cellular biological mechanisms are still largely unknown. Here we report an optical-tweezers-based method chirality frequency rotation. According intensity distribution patterns off-focus micron-size particles, established a judge orientation head along optical axis in trap. Together with rotation direction projection head, can...
The quality of quantitative differential phase contrast reconstruction (qDPC) can be severely degenerated by the mismatch background two oblique illuminated images, yielding problematic recovery results. These mismatches may result from illumination patterns, inhomogeneous media distribution, or other defocusing layers. In previous reports, is manually calibrated which time-consuming, and unstable, since new calibrations are needed if any modification to optical system was made. It also...
Quantitative differential phase contrast (qDPC) imaging has become an important method of optical measurement and life science research in microscopy because its high reconstruction resolution non-invasive, high-contrast quantitative biological samples. Despite the continuous development principle algorithm, frame rate existing qDPC algorithm is still much lower than that camera acquisition, so it hardly applied to real-time image fast-moving In this paper, based on color-coded multiplexing...
Abstract Sperm quality analysis plays an important role in diagnosing infertility, which is widely implemented by computer‐assisted sperm (CASA) of sperm‐swimming imaging from commercial phase‐contrast microscopy. A well‐equipped microscope comes with a high cost, increasing the burden assessment, and it also occupies large volume. For point‐of‐care testing (POCT) quality, these factors are confronted challenges low‐cost portable instruments. In this study, encoded light‐emitting diode (LED)...
In this research, we reveal the inborn but hitherto ignored properties of quantitative differential phase contrast (qDPC) imaging: transfer function being an edge detection filter. Inspired by this, highlighted duality qDPC between optics and pattern recognition, propose a simple effective reconstruction algorithm, termed Pupil-Driven (pd-qDPC), to facilitate quality for family qDPC-based algorithms. We formed new cost in which modified L0-norm was used represent pupil-driven sparsity,...
Differential phase contrast (DPC) imaging plays an important role in the family of quantitative measurement.However, reconstruction algorithm for DPC (qDPC) is not yet optimized, as it does incorporate inborn properties qDPC imaging.In this research, we propose a simple but effective image prior, dark-field sparse prior (DSP), to facilitate quality all DPC-based algorithms.The DSP based on key observation that most pixel values idea differential are zeros since subtraction two images under...
Measurement of the sample temperature in biophysics research is challenging, as samples are commonly placed a miniature chamber under microscope. In this study, we proposed method to measure an aqueous solution chambers microscopic system. Existing studies show that absorption coefficient spectrum water shifts with temperature, especially near-infrared (NIR) band. We measured spectra different temperatures and analyzed them, build mathematical model relating spectrum. A setup for measurement...
Differential phase contrast (DPC) imaging plays an important role in the family of quantitative measurement. However, reconstruction algorithm for DPC (qDPC) is not yet optimized, as it does incorporate inborn properties qDPC imaging. In this research, we propose a simple but effective image prior, dark-field sparse prior (DSP), to facilitate quality all DPC-based algorithms. The DSP based on key observation that most pixel values idea differential are zeros since subtraction two images...
The quality of quantitative differential phase contrast reconstruction (qDPC) can be severely degenerated by the mismatch background two oblique illuminated images, yielding problematic recovery results. These mismatches may result from illumination patterns, inhomogeneous media distribution, or other defocusing layers. In previous reports, is manually calibrated which time-consuming, and unstable, since new calibrations are needed if any modification to optical system was made. It also...