Fourier ptychographic microscopy (FPM) theoretically provides a solution to the trade-off between spatial resolution and field of view (FOV), has promising prospects in digital pathology. However, block reconstruction then stitching become an unavoidable procedure for large FOV due vignetting artifacts. This introduces artifacts, as existing image-domain optimization algorithms are highly sensitive systematic errors. Such obstacles significantly impede advancement practical implementation...

Point-of-care testing (POCT) plays an increasingly important role in biomedical research and health care. Quantitative phase microscopes (QPMs) with good contrast, no invasion, labeling, high speed automation could be effectively applied for POCT. However, most QPMs are fixed on the optical platform bulky size, lack of timeliness, which remained challenging POCT solutions. In this paper, we proposed a plug-and-play QPM multimode imaging based quantitative differential contrast (qDPC) method....

Abstract Computational microscopy enhances the space‐bandwidth product and corrects aberrations for high‐fidelity imaging by reconstructing complex optical wavefronts. Phase retrieval, a core technique in computational microscopy, faces challenges maintaining consistency between physical real‐world formation, as models idealize real phenomena. The discrepancy ideal actual formation limits application of especially non‐ideal situations. Here, feature‐domain achieving is introduced....

Optical tweezers provide a powerful tool to trap and manipulate living cells, which is expected help people gain physiological insights at single-cell level. However, trapping manipulating single cells under crowded environments, such as blood vessels lymph nodes, still challenging task. To overcome this issue, an annular beam formed by the far-field Bessel introduced serve optical shield isolate target from being disturbed. With scheme, we successfully trapped manipulated in environment....

We present a simple but efficient and robust reconstruction algorithm for Fourier ptychographic microscopy, termed error-laxity iterative engine (Elfpie), that is simultaneously to (1) noise signal (including Gaussian, Poisson, salt & pepper noises), (2) problematic background illumination problem, (3) vignetting effects (4) misaligning of LED positions, without the need calibrating or recovering these system errors. In Elfpie, we embed inverse problem FPM under framework feature...

We present deep-ultraviolet Fourier ptychography (DUV-FP) for high-resolution chemical imaging of biological specimens in their native state without exogenous stains. This approach uses a customized 265-nm DUV LED array angle-varied illumination, leveraging the unique absorption properties biomolecules at this wavelength region. implemented robust feature-domain optimization framework to overcome common challenges ptychographic reconstruction, including vignetting, pupil aberrations, stray...

This study introduces a novel image formation model - the double pass fundus reflection (DPFR) for retinal enhancement (restoration). The DPFR reveals specific feature that was hitherto neglected in modeling light propagation of imaging all published reports on enhancement. Based model, procedures proposed restoration algorithm are given. failure dark channel prior images RGB color space is clarified. While solution about how to bypass challenge proposed. Each step tested experimentally with...

Quantitative differential phase contrast (qDPC) microscopy can achieve imaging of unlabeled cell samples with high spatial resolution. However, qDPC is easily affected by the noise generated in experiments. In this manuscript, L0-norm regularization was introduced into reconstruction for sparse (L0-qDPC), including quantitative target and samples. After detailed comparison fidelity, contrast, resolution, convergence rate, L0-qDPC method provide a stable without parameter adjustment due to...

Fourier ptychographic microscopy (FPM), characterized by high-throughput computational imaging, theoretically provides a cunning solution to the trade-off between spatial resolution and field of view (FOV), which has promising prospect in application digital pathology. However, block reconstruction then stitching currently become an unavoidable procedure due vignetting effects. The stitched image tends present color inconsistency different segments, or even artifacts. In response, we...

Abstract A weak codon usage bias was found in Dendrobium catenatum ( D. officiale ) antimicrobial peptides (AMPs), after the analysis of relative synonymous usage, GC contents, and effective number codons. The preference mainly influenced by natural selection pressure. self‐optimized prediction method SWISS‐MODEL were applied for peptide structural domain analyses, some typical domains officinale AMP amino sequences, such as knot1 domain, gibberellins‐stimulated cupin_1 defensin_like SLR1‐BP...

Abstract Fourier ptychographic microscopy (FPM) breaks through the resolution limitations of conventional optical systems, which offer a full‐field view and high without additional mechanical scanning. However, image‐domain optimizations require trade‐offs between correction efficacy, data redundancy, reconstruction accuracy. Furthermore, existing linear time‐invariant model for actual nonlinear, time‐varying systems leads to forward mismatch, complicating corrections vignetting effect. To...

In this research, we studied the duality between cataractous retinal image dehazing and denoising proposed that task for images can be achieved with combination of sigmoid function. To do so, introduce double-pass fundus reflection model in YPbPr color space developed a multilevel stimulated strategy termed MUTE. The transmission matrix cataract layer is expressed as superposition denoised raw different levels weighted by pixel-wise functions. We further designed an intensity-based cost...

Phase unwrapping is widely used in interferometry. In the typical least square (LS) method, noise wrapped phase cannot be removed. An improved algorithm based on total variation (TV) imposes L1-norm image gradient to improve sparsity. However, it may lead over-sparsity, and unwrapped will compressed if penalty term overlarge. this study, we proposed a sparse prior (SPUP) solve of interferograms diffraction microscopy (DPM). Since DPM has unique sample sparsity imaging field high-power...

We have studied the characteristics of nonparaxial abruptly autofocusing beams (AABs) with structured polarization states. Based on relationship between rays and their pre-engineered circular caustics, closed-form Fourier spectra these are derived, electric fields represented by superposition polarized plane waves. The simulation results show that such various shapes focal spots, including dot-like, doughnut-like, other array-like which depend state beams. Moreover, for AABs a high numerical...

To analyze the target and potential mechanism of Scutellaria baicalensis (SB) in treatment HCC based on bioinformatics, so as to provide suggestions for diagnosis, treatment, drug development hepatocellular carcinoma (HCC). The regulated gene targets SB were screened by expression pattern clustering differential analysis data HepG2 cells treated with at 0 h, 1 3 6 12 24 h. module genes related identified weighted coexpression network (WGCNA). KEGG GO enrichment used molecular function...

The ray-optics (RO) model is a reasonable method to calculate optical force in geometrical optics regime. However, the RO fails produced by diffractive field and other arbitrary structured light beams. We propose Fourier ray (FR) for incident Combining optics, FRs are defined as rays that inlay on plane waves weighted angular spectrum of beam. According traditional FR method, we can analyze forces microsphere immersed various To validate fundamental Gaussian beam Airy respectively calculated...

The quality of quantitative differential phase contrast reconstruction (qDPC) can be severely degenerated by the mismatch background two oblique illuminated images, yielding problematic recovery results. These mismatches may result from illumination patterns, inhomogeneous media distribution, or other defocusing layers. In previous reports, is manually calibrated which time-consuming, and unstable, since new calibrations are needed if any modification to optical system was made. It also...

Many ray-optics models have been proposed to describe the propagation of paraxial Gaussian beam. However, those are inapplicable beams that violate condition. In this paper, we present a skew line ray (SLR) based model represent properties nonparaxial beam under oblate spheroidal coordinates. The free-space evolution complex wavefront light including amplitude and phase is derived via model. Our analysis demonstrates SLR available for both conditions, can be used precisely wavefront....

vertical takeoff and landing aircrafts are widely concerned due to its unique advantages. In this paper, technical characteristics development trends of thrust vector (VTOL) aircrafts, tilted rotor flight power systems introduced, based on the existing aircraft. Design methods control studied unmanned aerial vehicle (UAV). Developments aircraft prospected in paper.

In the ray-optics (RO) model of optical tweezers, tracing refractive and reflected rays with vectors play important roles in calculating trapping forces. Traditional ray-tracing method solid geometry, to some extent, is complicated determining orientations those according spatial incident rays. It difficult calculate forces for irregular particles. this paper, quaternion proposed rotate ray simplifying traces all kinds Then, it appropriate force an ellipsoid bead. Based on algorithm...