A5024930317- Analytical chemistry methods development
- Selenium in Biological Systems
- Heavy metals in environment
- Heavy Metal Exposure and Toxicity
- Trace Elements in Health
- Advanced Glycation End Products research
- Heavy Metals in Plants
- Analytical Chemistry and Chromatography
- Epigenetics and DNA Methylation
- Mercury impact and mitigation studies
- Water Quality Monitoring and Analysis
- Plant Stress Responses and Tolerance
- Pesticide Residue Analysis and Safety
- Adsorption and biosorption for pollutant removal
- Chromium effects and bioremediation
- RNA modifications and cancer
- Electrochemical Analysis and Applications
- Mass Spectrometry Techniques and Applications
- Spectroscopy and Chemometric Analyses
- Analytical Methods in Pharmaceuticals
- Advanced Chemical Sensor Technologies
- Fermentation and Sensory Analysis
- Dye analysis and toxicity
- Arsenic contamination and mitigation
- Fatty Acid Research and Health
Universidad de Guanajuato
2016-2025
University of Cincinnati
2002-2011
Agilent Technologies (United States)
2005-2011
Universidad de Oviedo
1994-1996
Medical University of Białystok
1990
Significance Significant progress has been achieved in our understanding of plant adaptive responses to ensure growth and reproduction soils with low phosphate (Pi) availability. However, the potential role epigenetic mechanisms modulation these remains largely unknown. In this article, we describe dynamic changes global DNA methylation patterns that occur Arabidopsis plants exposed Pi availability; are associated onset starvation responses. We show expression a subset Pi-responsive genes is...
Plant cells have the capacity to generate a new plant without egg fertilization by process known as somatic embryogenesis (SE), in which differentiated can form embryos able functional plant. Although there been advances understanding genetic basis of SE, epigenetic mechanism that regulates this is still unknown. Here, we show embryogenic development Coffea canephora proceeds through crosstalk between DNA methylation and histone modifications during earliest stages SE. We found low levels...
Brazil nuts have been classified as the foodstuffs that contain highest level of unadulterated selenium, an essential trace element appears to prevent cancer. To date, characterization selenium species in brazil has not yet investigated. In this work, various sample preparation approaches, including microwave extractions and enzymatic treatments, are examined with goal preservation subsequent speciation; these treatment Proteinase K proved most effective. High-performance liquid...
The augmented consumption of dietary advanced glycation end products (dAGEs) has been associated with increased oxidative stress and inflammation, however, there is insufficient information over the effect on insulin resistance. objective present study to investigate dAGEs restriction tumor necrosis factor-α (TNF-α), malondialdehyde, C-reactive protein (CRP), resistance in DM2 patients. We carried out a randomized 6 weeks prospective two groups patients: subjects standard diet (n = 13), vs...
Somatic embryogenesis is a powerful biotechnological tool for the mass production of economically important cultivars. Due to cellular totipotency plants, somatic cells under appropriate conditions are able develop complete functional embryo. During induction embryogenesis, there different factors involved in success or failure response. Among these factors, origin explant, culture medium and vitro environmental have been most studied. However, secretion molecules into media has not fully...
Abnormal fatty acid metabolism and availability are landmarks of metabolic diseases, which in turn associated with aberrant DNA methylation profiles. To understand the role acids disease epigenetics, we sought profiles specifically induced by arachidonic (AA) or oleic (OA) cultured cells compared those published normal diseased tissues. THP-1 monocytes were stimulated AA OA analyzed using Infinium HumanMethylation450 BeadChip (Illumina) Human Exon 1.0 ST array (Affymetrix). Data corroborated...
In eukaryotes, actual DNA methylation patterns provide biologically important information, for which both, genome-wide and locus-specific at cytosine residues have been extensively studied. The original contribution of this work relies on the selective derivatization moieties with 2-bromoacetophenone determination global by reversed phase high performance liquid chromatography spectrofluorimetric detection. features proposed procedure are as follows: (1) no need elimination RNA, (2)...
Two liquid chromatography procedures with PPO as IS were set up and implemented for the determination of eight fungicides in tanned leather; both suitable analytical control industrial products.
Glyoxylic acid (GA) is a human metabolite potentially involved in glycation processes and considered precursor of Nε-carboxymethyl lysine (CML). Different mechanisms GA reaction with chemically protected at...
Abstract The antioxidant properties of Se nanoparticles (SeNPs) have been frequently observed in biological systems. Reduced levels hydrogen peroxide reported, but it remains unclear whether this effect is due to the increased activity enzymes caused by SeNPs or may be result their direct involvement reduction process. This work demonstrates ability reduce H 2 O with concomitant oxidation elemental Se(VI) at physiological pH and presence major serum protein. (96 ± 8 nm) were obtained from...
Abstract We proposed a simple analytical procedure for measurement of serum advanced glycosylation end products (AGEs) based on simultaneous detection low-molecular-mass peptides and AGEs with flow system two detectors connected on-line: spectrophotometric (λ = 280 nm) spectrofluorometric (λex 247 nm, λem 440 nm). Sample pretreatment was carried out in microcentrifuge tubes: Serum (20 μL) deproteinized trichloroacetic acid (480 μL, 0.15 mol/L) lipids were extracted chloroform (100 μL)....
Our aim was to investigate the adverse effects of occupational exposure trivalent chromium. We measured chromium and iron levels in serum urine hemoglobin tannery workers unexposed persons. studied three groups subjects. Group 1 included 15 non-smoking male highly exposed from tanning retanning departments. 2 14 with moderate dying, drying finishing 3 11 healthy, subjects without direct exposure. Higher were observed respect group (mean values respectively: 0.43; 0.25 0.13 μg•l-1). Urine...
Abstract Fatty acids (FA) modify DNA methylation in vitro , but limited information is available on whether corresponding associations exist vivo and reflect any short-term effect of the diet. Associations between global FAs were sought blood from lactating infants (LI; n = 49) adult males (AMM; 12) equally distributed across three conventional BMI classes. AMM provided multiple samples at 2-hour intervals during 8 hours after either a single Western diet-representative meal (post-prandial...