- Analytical chemistry methods development
- Selenium in Biological Systems
- Heavy Metal Exposure and Toxicity
- Fungal Biology and Applications
- Advanced Glycation End Products research
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Plant Stress Responses and Tolerance
- Heavy Metals in Plants
- Fermentation and Sensory Analysis
- Biochemical and biochemical processes
- Heavy metals in environment
- Antifungal resistance and susceptibility
- Mercury impact and mitigation studies
- Trace Elements in Health
- Plant Pathogens and Fungal Diseases
- Biochemical effects in animals
- Fungal and yeast genetics research
- Metabolomics and Mass Spectrometry Studies
- Plant-Microbe Interactions and Immunity
- Electrochemical Analysis and Applications
- Pesticide Residue Analysis and Safety
- Microbial Natural Products and Biosynthesis
- Phytochemicals and Antioxidant Activities
- Arsenic contamination and mitigation
Universidad de Guanajuato
2016-2025
Institute for Scientific and Technological Research
2022
Two liquid chromatography procedures with PPO as IS were set up and implemented for the determination of eight fungicides in tanned leather; both suitable analytical control industrial products.
Glyoxylic acid (GA) is a human metabolite potentially involved in glycation processes and considered precursor of Nε-carboxymethyl lysine (CML). Different mechanisms GA reaction with chemically protected at...
Abstract The antioxidant properties of Se nanoparticles (SeNPs) have been frequently observed in biological systems. Reduced levels hydrogen peroxide reported, but it remains unclear whether this effect is due to the increased activity enzymes caused by SeNPs or may be result their direct involvement reduction process. This work demonstrates ability reduce H 2 O with concomitant oxidation elemental Se(VI) at physiological pH and presence major serum protein. (96 ± 8 nm) were obtained from...
An ultrasound accelerated, environmentally benign Ugi-azide based method was developed for the synthesis of 1,5-disubstituted tetrazoles under solvent and catalyst-free conditions.
HPLC-HG-MP-AES procedure was developed for the determination of Se(<sc>iv</sc>) and SeMet in Se-yeast.
Abstract Mucormycosis is a fungal infection caused by Mucorales, with high mortality rate. However, only few virulence factors have been described in these organisms. This study showed that deletion of rfs , which encodes the enzyme for biosynthesis rhizoferrin, siderophore, Mucor lusitanicus led to lower diabetic mice and nematodes. Upregulation correlated increased toxicity cell-free supernatants culture broth (SS) obtained under growing conditions favor oxidative metabolism, such as low...
Secondary metabolites are crucial for the establishment of interactions between plants and microbes, as in case Trichoderma-plant interactions. In biosynthetic pathway secondary metabolites, specific enzymes participate formation hydroxyl epoxy groups, belonging to p450 monooxygenases family. Here, we show that product gene TvCyt2 from Trichoderma virens encodes a new protein homologous cytochrome p450, which is down-regulated at beginning Trichoderma-Arabidopsis interaction. To investigate...
In this work, the effect of cadmium (0–5.0 mg L−1 as chloride, Cd(ii)) and selenium (0–2.0 sodium selenite, Se(iv)) was studied in Lepidium sativum with specific focus on glyoxal (GO) methylglyoxal (MGO) cellular distribution both elements under different exposure conditions. The concentrations two reactive α-ketoaldehydes present natural metabolites by-products lipid peroxidation, were increased plants treated Cd(ii), providng complementary experimental evidence element phytotoxicity garden...
Determination and speciation of As in tortillas are reported.
Liquid chromatography-high-resolution mass spectrometry was used for the first time to investigate impact of Se(IV) (10 mgSe L-1 as sodium selenite) on Allium cepa L. root proteome. Using MaxQuant platform, more than 600 proteins were found; 42 identified based at least 2 razor + unique peptides, score > 25, and found be differentially expressed in exposed versus control roots with t-test difference ±0.70 (p < 0.05, Perseus). caused growth inhibition decrease total RNA roots. Different...
Microwave plasma - atomic emission spectrometry with multi-energy calibration (MP-AES-MEC) was used for the determination of four major elements in urine. In family spectrometry, distinctive features MP-AES are: (i) nitrogen-based toroidal-shape plasma; (ii) good tolerance to total solids; (iii) exceptionally low operating cost. On other hand, due relatively temperature, this technique is susceptible spectral interferences and sample-to-sample fluctuating baseline typical limitation, if...
It has been reported that glycation of human serum albumin (HSA) changes its capability for copper binding whereas the increase free might have an impact on protein - a key process in diabetes progression. In this work, proteomic analysis non-glycated HSA and glycated with methylglyoxal (MGo) absence or presence Cu(ii) (0.1; 1.0; 5.0 mg Cu L-1) undertaken. Trypsin hydrolysates were subjected to capillary HPLC-ESI-QTOF-MS MS/MS. Raw data analyzed using two platforms: MaxQuant () ProteinScape...