- Entomopathogenic Microorganisms in Pest Control
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Insect symbiosis and bacterial influences
- Fungal and yeast genetics research
- Insect Resistance and Genetics
- Fermentation and Sensory Analysis
- Fungal Biology and Applications
- Plant and soil sciences
- Mycorrhizal Fungi and Plant Interactions
- Peroxisome Proliferator-Activated Receptors
- Polyamine Metabolism and Applications
- Botanical Research and Applications
- Plant Pathogens and Fungal Diseases
- Microbial Metabolites in Food Biotechnology
- Nematode management and characterization studies
- RNA Research and Splicing
- Invertebrate Immune Response Mechanisms
- Microbial bioremediation and biosurfactants
- Enzyme Catalysis and Immobilization
- Chromium effects and bioremediation
- Enzyme Production and Characterization
- Amino Acid Enzymes and Metabolism
- RNA regulation and disease
- Plant Parasitism and Resistance
Universidad de Guanajuato
2013-2023
York University
2018
University of Alberta
2003
Universidad de Salamanca
1997
Instituto Politécnico Nacional
1996
Center for Research and Advanced Studies of the National Polytechnic Institute
1996
The peroxin Pex23p of the yeast Yarrowia lipolytica exhibits high sequence similarity to hypothetical proteins Ylr324p, Ygr004p, and Ybr168p encoded by Saccharomyces cerevisiae genome. are integral peroxisomal membrane act control peroxisome number size. Synthesis Ylr324p Ybr168p, but not is induced during incubation cells in oleic acid-containing medium, metabolism which requires intact peroxisomes. Cells deleted for YLR324w exhibit increased numbers peroxisomes, whereas YGR004w or YBR168w...
Transcriptome profiling identified the gene PEX25 encoding Pex25p, a peroxisomal membrane peroxin required for regulation of peroxisome size and maintenance in Saccharomyces cerevisiae. Pex25p is related to protein unknown function encoded by open reading frame, YOR193w, S. cerevisiae genome. Yor193p peripheral that exhibits high sequence similarity not only but also Pex11p. Unlike Pex11p, constitutively expressed wild-type cells grown oleic acid-containing medium, metabolism which requires...
The peroxin Pex24p of the yeast Yarrowia lipolytica exhibits high sequence similarity to two hypothetical proteins, Yhr150p and Ydr479p, encoded by Saccharomyces cerevisiae genome. Like YlPex24p, both Ydr479p have been shown be integral peroxisomal membrane, but unlike their levels synthesis are not increased upon a shift cells from glucose- oleic acid–containing medium. Peroxisomes deleted for either or YHR150w YDR479c genes in number, exhibit extensive clustering, smaller area than...
Invertebrate immune priming is a growing field in immunology. This phenomenon refers to the ability of invertebrates generate more vigorous response second encounter with specific pathogen and can occur within across generations. Although precise mechanism has not been elucidated, it suggested that methylation DNA cornerstone for this phenomenon. Here, using novel method analytical chemistry (a reversed-phase liquid chromatography procedure) beetle Tenebrio molitor as model system, we did...
HPLC-HG-MP-AES procedure was developed for the determination of Se(<sc>iv</sc>) and SeMet in Se-yeast.
The dimorphic fungus Yarrowia lipolytica grows to form hyphae either in rich media or with GlcNAc as a carbon source. A visual screening, called FIL (filamentation minus), for Y. yeast growth mutants has been developed. screen was used identify three genes that abolish hypha formation all assayed. HOY1, gene whose deletion prevents the yeast-hypha transition both liquid and solid media, characterized. HOY1 is predicted encode 509-amino-acid protein homeodomain homologous found chicken Hox4.8...
Insect pathogenic fungi such as Metarhizium anisopliae and Beauveria bassiana have an increasing role in the control of agricultural insect pests vectors human diseases. Many virulence factors are well studied but less is known metabolism these during course infection or saprobic growth. Here, we assessed enzyme activity gene expression central carbon metabolic pathway, including isocitrate dehydrogenase, aconitase, citrate synthase, malate synthase (MLS) lyase (ICL), with particular...
The dimorphic yeast Yarrowia lipolytica is used as a model to study fungal differentiation because it grows yeast-like cells or forms hyphal in response changes environmental conditions. Here, we report the isolation and characterization of gene, ZNC1, involved transition Y. lipolytica. ZNC1 gene encodes 782 amino acid protein that contains Zn(II)2C6 fungal-type zinc finger DNA-binding domain leucine zipper domain. transcription elevated during growth decreases formation mycelium. Cells...
The American pokeweed plant, Phytolacca americana, displays broad-spectrum resistance to plant viruses and is a heavy metal hyperaccumulator. However, little known about the regulation of biotic abiotic stress responses in this non-model plant. To investigate control miRNAs gene expression, we sequenced small RNA transcriptome treated with jasmonic acid (JA), hormone that mediates pathogen defense tolerance. We predicted 145 responsive JA, most which were unique pokeweed. These low abundance...
Beneficial associations are very important for plants and soil-dwelling microorganisms in different ecological niches, where communication by chemical signals is relevant. Among the signals, release of phytohormones to establish beneficial with fungi, a recently described association that entomopathogenic ascomycete fungus Metarhizium plants. Here, we evaluated effect four phytohormones, synthetic strigolactone (GR24), sorgolactone (SorL), 3-indolacetic acid (IAA) gibberellic (GA3), on...