A5079912873- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Immunodeficiency and Autoimmune Disorders
- Chronic Lymphocytic Leukemia Research
- Microfluidic and Capillary Electrophoresis Applications
- Lymphoma Diagnosis and Treatment
- Virus-based gene therapy research
- Microbial Metabolic Engineering and Bioproduction
- T-cell and Retrovirus Studies
- Higher Education and Sustainability
- Botanical Research and Chemistry
- Phosphodiesterase function and regulation
- DNA Repair Mechanisms
- Nanoparticle-Based Drug Delivery
- Viral Infections and Immunology Research
- Antimicrobial Peptides and Activities
- Bacterial Genetics and Biotechnology
- Latin American Urban Studies
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- DNA and Nucleic Acid Chemistry
- Organizational Management and Innovation
- Business, Education, Mathematics Research
- Gene Regulatory Network Analysis
Institut Pasteur de Montevideo
2018-2024
Institut Pasteur
2014
Consejo Nacional de Investigaciones Científicas y Técnicas
2014
International Centre for Genetic Engineering and Biotechnology
2002-2005
Recombinant protein expression has become an invaluable tool for academic and biotechnological projects. With the use of high-throughput screening technologies soluble production, uncountable target proteins were produced in a homogeneous state enabling realization further studies. Evaluation hundreds conditions requires cloning methods. Here we describe new versatile vector suite dedicated to improvement recombinant with solubility problems. This allows parallel same PCR product into 12...
The Ku heterodimer plays a central role in non-homologous end-joining. binding of recombinant to DNA has been investigated by dynamic light scattering, double-filter binding, fluorescence spectroscopy, and band shift assays. hydrodynamic radius solution is 5.2 nm does not change when 25-bp double-strand (dsDNA) fragment (D25) added, indicating that only one molecule binds fragment. dissociation constant (<i>k</i> <sub>d</sub>) for the D25 3.8 ± 0.9 nm. If both ends substrate are closed with...
Recombinant protein expression has become an invaluable tool in basic and applied research. The accumulated knowledge this field allowed the of thousands targets a soluble, pure homogeneous state, essential for biochemical structural analyses. A lot progress been achieved last decades, where challenging proteins were expressed soluble manner after evaluating different parameters such as host, strain, fusion partner or promoter strength, among others. In regard, we have previously developed...
The detection of small molecules in living cells using genetically encoded FRET sensors has revolutionized our understanding signaling pathways at the sub-cellular level. However, engineering fluorescent proteins and specific binding domains to create new remains challenging because difficulties associated with large size polypeptides involved, their intrinsically huge conformational variability. Indeed, sensors’ design still relies on vague structural notions, trial error combinations...
The COVID-19 pandemic evolves constantly, requiring adaptable solutions to combat emerging SARS-CoV-2 variants. To address this, we created a pentameric scaffold based on mammalian protein, which can be customized with up 10 protein binding modules. This molecular spans roughly 20 nm and simultaneously neutralize Spike proteins from one or multiple viral particles. Using only two different modules targeting the Spike's RBD domain, this construct outcompetes human antibodies vaccinated...
An on-line preconcentration capillary electrophoresis (CE) methodology, which combines a normal stacking mode (NSM) with dynamic pH junction technique, is introduced in this paper. A systematic study of the parameters (concentration and value background electrolyte, composition sample matrix, injection pressure time, separation voltage temperature) affecting concentration seven related phenolic compounds was investigated optimized. Under optimum focusing conditions, about 54-fold improvement...
ABSTRACT Bovine leukemia virus (BLV) is a B-lymphotropic oncogenic retrovirus of the genus Deltaretrovirus that infects dairy cattle worldwide and causative agent enzootic bovine leukosis. BLV demonstrates remarkably low efficiency in infecting cells via free viral particles derived from infected B cells, as virions are rarely detected bloodstream cattle. However, transmission efficacy significantly increases upon establishment direct cell-to-cell interactions. Syncytium formation assays...