A5040891677- Toxoplasma gondii Research Studies
- Parasitic Infections and Diagnostics
- Coccidia and coccidiosis research
- Helminth infection and control
- Herpesvirus Infections and Treatments
- Vector-borne infectious diseases
- Microbial infections and disease research
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Infant Nutrition and Health
- Parasitic Diseases Research and Treatment
- Animal health and immunology
- Bartonella species infections research
- Yersinia bacterium, plague, ectoparasites research
- Insect and Pesticide Research
- Rabbits: Nutrition, Reproduction, Health
- Polyamine Metabolism and Applications
- Parasitic infections in humans and animals
- Viral gastroenteritis research and epidemiology
- Animal Nutrition and Physiology
- Syphilis Diagnosis and Treatment
- Aquaculture disease management and microbiota
- Dermatological diseases and infestations
- Antibiotics Pharmacokinetics and Efficacy
- Pneumonia and Respiratory Infections
The Ohio State University
2015-2024
University of Missouri
1998-2008
Western University of Health Sciences
2008
Kansas State University
2008
American Veterinary Medical Association
2008
Washington State University
2007
Peterson & Smith Equine Hospital
2007
Missouri College
1998-2005
Arkansas Agricultural Experiment Station
2004
University of California, Davis
1992-2002
Neospora hughesi n. sp. was isolated from the central nervous system tissue of an adult equine (Equus caballus) California. The tachyzoites are crescent-shaped, approximately 2 x 5 microm (1.8-3.0 4.0-7.0 microm), with characteristic apical complex structures consisting anterior polar ring, conoid, numerous rhoptries filled a uniform electron-dense material, and 22 microtubules extending posteriorly ring. Comparison N. to canine bovine caninum isolates showed phenotypic differences in...
Neosporosis was diagnosed in an 11-year-old Quarter Horse gelding with clinical signs and diagnostic test results compatible equine protozoal myeloencephalitis (EPM). Presumptive postmortem diagnosis of EPM attributable to Sarcocystis neurona infection is generally made on the basis detecting antibody titer S CSF or characteristic histologic lesions, even when parasites have not been specifically identified. confirmed horse described here by use immunohistochemical examination, vitro...
ABSTRACT Current serologic tests used to detect antibodies Neospora caninum require species-specific secondary antibodies, limiting the number of species that can be tested. In order examine a wide variety animal may infected with N. , modified direct agglutination test (N-MAT) similar Toxoplasma gondii (T-MAT) was developed. This measures parasites by -specific in serum, thus eliminating need for host-specific anti-isotype sera. The N-MAT compared indirect fluorescent-antibody (IFAT) and...
This is a report of the isolation and in vitro propagation merozoites Sarcocystis falcatula. Culture-derived S. falcatula remained infectious after several passages tissue culture as determined by susceptibility experimentally inoculated Melopsittacus undulatus that died pulmonary sarcocystosis experimental inoculation. There was no obvious clinical disease or lesion when neurona were used to inoculate M. undulatus. On basis biological infectivity data, are not synonymous suggested from...
Equine protozoal myeloencephalitis (EPM) is a neurologic disease of horses caused by Sarcocystis neurona. The horse dead-end host for S. neurona and the definitive intermediate hosts have not previously been identified. We hypothesized that actually falcatula, parasite cycles in nature between Virginia opossums (Didelphis virginiana) any variety avian hosts. extracted DNA from falcatula sarcocysts muscle brown-headed cowbird (Molothrus ater) schizonts fixed specimen lung Moluccan cockatoo...
Seven Pacific harbor seals with meningoencephalitis associated Sarcocystis neurona-like protozoa are described. Six of the 7 were free-ranging and found stranded over an 80-km stretch central California coastline; other was captive. All had marked to severe nonsuppurative meningoencephalitis, most in cerebellar cortex. Immunohistochemistry for S. neurona antigens positive on brain tissue all cases, revealing numerous merozoites as well developing mature schizonts, including rosette forms....
The genetic diversity among 6 Sarcocystis falcatula isolates derived from geographically distinct regions in the U.S.A. was detected using first internal transcribed spacer region 1 (ITS-1) of rRNA gene. These sequences were then compared to full sequence a neurona isolate obtained California horse diagnosed with equine protozoal myeloencephalitis. No nucleotide differences over partial analysis 2 additional S. isolates: however, complete for ITS-1 not compared. Twelve consistently when...
Neospora is a newly recognized genus of pathogenic coccidia, closely related to Toxoplasma gondii, that can cause abortion or congenital disease in variety domestic animal hosts. On the basis small-subunit rRNA gene sequences spp. and other apicomplexa oligonucleotide primers COC-1 COC-2 were used for PCR amplification conserved approximately 300 bp size. A Neospora-specific chemiluminescent probe hybridized Southern blots products from DNA but not with amplified coccidian parasites tested....
Abstract Objectives To identify Sarcocystis neurona -specific DNA sequences in the nuclear small subunit ribosomal RNA (nss-rRNA) gene that could be used to distinguish S from other closely related protozoal parasites, and evaluate a polymerase chain reaction (PCR) test, using broad based primers unique species-specific probe on CSF for detection of equids. Procedures Sequencing new isolate (UCD 1) was performed. The sequence compared with Sarcocystidae parasites. From this sequence,...
An unidentified Sarcocystis falcatula-like infection was diagnosed in a captive bee-eater (Merops nubicus) zoo Florida. The bird died suddenly, probably due to protozoa-associated pneumonia. Protozoal schizonts were found lungs and heart, immature sarcocysts seen skeletal muscles. Ultrastructurally, located capillary endothelium merozoites lacked rhoptries, consistent with the structure of species. Sarcocysts immature, microscopic, contained only metrocytes. sarcocyst wall had finger-like...
Ancylostoma caninum is a nematode of the canine gastrointestinal tract commonly referred to as hookworm. This study involved eight privately owned adult greyhounds presenting with persistent A. ova shedding despite previous deworming treatments. The dogs received combination treatment protocol comprising topical moxidectin, followed by pyrantel/febantel/praziquantel within 24 hr. At 7-10 days posttreatment, fecal examination monitored for parasite ova. Dogs remained on monthly until they...
The diagnosis and management of canine heartworm disease is a growing concern for shelter veterinarians. Although the accuracy commercial antigen test kits has been widely studied, recent reports have renewed interest in blocking as causative factor false "no detected" results. objectives this study were to determine prevalence results adult dogs entering shelters northern, southern, western regions country identify historical clinical risk factors such Serum samples evaluated Dirofilaria...
Abstract Background Companion animal endoparasites play a substantial role in both veterinary medicine and public health. Updated epidemiological studies are necessary to identify trends occurrence distribution of these parasites, their associated risk factors. This study aimed assess the canine retrospectively, using fecal flotation test data available through participating academic parasitology diagnostic laboratories across United States America (USA). Methods Canine records from ten...
Fatal hepatic sarcocystosis was diagnosed in 2 polar bears from a zoo Anchorage, Alaska. Gross lesions were icterus and systemic petechiae. Marked microscopic detected only the liver included severe random necrotizing hepatitis with hemorrhage. Only asexual stages of an apicomplexan parasite within hepatocytes, rare extracellular zoites seen foci necrosis. The divided by endopolygeny, occasionally merozoites formed rosettes around central residual body. Ultrastructural features conoid low...