human granulocytic ehrlichiosis is a potentially fatal tick-borne infection that has recently been described. This acute febrile illness characterized by myalgias, headache, thrombocytopenia, and elevated serum aminotransferase levels. The disease difficult to diagnose because the symptoms are nonspecific, intraleukocytic inclusions (morulae) may not be seen, serologic results often initially negative. Little known about causative agent it never cultivated.

Homology in the 16S rDNAs shows that agent of human granulocytic ehrlichiosis (HGE) is closely related to veterinary pathogens Erlichia equi and phagocytophila. After HGE, patients develop antibodies reactive with E. phagocytophila; thus, we hypothesized these species are share significant antigenicity. Antisera from humans, horses, dogs, cattle were tested by indirect fluorescent-antibody assay (IFA) for other ehrlichiae immunoblot identify specific reactions equi. All convalescent-phase...

Summary Case records of horses with equine ehrlichiosis (Ehrlichia equi) at the University California Veterinary Medical Teaching Hospital and Ackerman Creek Large Animal Clinic were analyzed for evaluation clinical signs, time onset, hematologic values, response to treatment, recovery. Equine was found be seasonal in foothills northern California, higher incidence than reported previously. The developed fever, anorexia, depression, limb edema, icterus, ataxia. Hematologic changes...

Pituitary pars intermedia dysfunction is a slowly progressive disorder that afflicts most breeds of horses. Because it shares features with human Cushing disease, has been referred to as equine disease. A variety tests pituitary-adrenocortical function were performed on horses evidence pituitary intermediate dysfunction, and results compared those in healthy control Diurnal variations plasma cortisol concentration not statistically different between dysfunction. An ACTH stimulation (1 U...

Ehrlichia equi, a rickettsia described from horses in California 30 yr ago, causes equine granulocytic ehrlichiosis throughout the Americas and possibly Europe. Here, we report experimental transmission of E. equi infected to susceptible through bites western blacklegged ticks, Ixodes pacificus (Cooley & Kohls). In preliminary field studies, only I. pacificiis consistently infested vegetation at 3 locations with contemporary cases ehrlichosis, particular, was species found attached all...

Neosporosis was diagnosed in an 11-year-old Quarter Horse gelding with clinical signs and diagnostic test results compatible equine protozoal myeloencephalitis (EPM). Presumptive postmortem diagnosis of EPM attributable to Sarcocystis neurona infection is generally made on the basis detecting antibody titer S CSF or characteristic histologic lesions, even when parasites have not been specifically identified. confirmed horse described here by use immunohistochemical examination, vitro...

The Ehrlichia phagocytophila-group also includes E. equi and the human granulocytic ehrlichiosis (HGE) agent that are probably a single species. Disease is mild to severe illness in ruminants, horses, humans, but comparative pathology ehrlichial distribution tissues poorly described. We compared humans with HGE, horses infection, sheep phagocytophila infection. Frequent findings included splenic lymphoid depletion, small macrophage aggregates apoptoses liver, paracortical hyperplasia lymph...

ABSTRACT Leptospiral organisms have long been presumed to be associated with the presence of equine recurrent uveitis. This project was undertaken determine Leptospira spp. in aqueous humor horses uveitis if there an association inflammation. Thirty were determined based on clinical evaluation or history. Sixteen judged clinically and historically free used as controls. Aqueous samples cultured evaluated by PCR for DNA. Serum collected antibodies against five serovars a leptospirosis panel....

ABSTRACT Human granulocytic ehrlichiosis (HGE) is a potentially fatal, tick-borne disease caused by bacterium related or identical to Ehrlichia phagocytophila . To identify and characterize E. group-specific protein antigen genes, we prepared screened HGE agent equi genomic DNA expression libraries using polyclonal equine antibodies. Two clones, one each from , that were recognized specifically antibodies the group ehrlichiae had complete open reading frames of 3,693 3,615 nucleotides,...

The equine granulocytic ehrlichiosis agent, Ehrlichia equi, is closely related or identical to the human (HGE) agent. Both are suspected of being transmitted by ticks. We have successfully isolated E. equi in a cell line, IDE8, derived from putative vector, tick Ixodes scapularis. Peripheral blood leukocytes an experimentally infected horse were inoculated onto IDE8 monolayers. Cultures incubated candle jar at 34 degrees C culture medium with NaHCO3 and organic buffer...

Borrelia burgdorferi infection is common in horses living Lyme endemic areas and the geographic range for exposure increasing. Morbidity after B. unknown. Documented, naturally occurring syndromes attributed to include neuroborreliosis, uveitis, cutaneous pseudolymphoma. Although other clinical signs such as lameness stiffness are reported horses, these often not well documented. Diagnosis of disease based on burgdorferi, cytology or histopathology infected fluid tissue antigen detection....

The agent of human granulocytic ehrlichiosis (HGE), Ehrlichia phagocytophila, and equi are very similar. HGE is variable severity. Genetic antigenic differences among 3 isolates (Webster, Spooner, NY-8) 1 horse isolate (MRK) were evaluated. 16S rRNA gene sequences identical in all isolates. By use 5 homologous antisera from these humans an additional heterologous reactions, the immunodominant antigens each noted to differ molecular size: 43 kDa Webster (Wisconsin) isolate, 46 Spooner 42 45...

Journal Article Transmission and Passage in Horses of the Agent Human Granulocytic Ehrlichiosis Get access John E. Madigan, Madigan Search for other works by this author on: Oxford Academic PubMed Google Scholar Philip J. Richter, Jr., Jr. Robert B. Kimsey, Kimsey Jeffrey Barlough, Barlough Johan S. Bakken, Bakken Reprints or correspondence: Dr. Dept. Medicine Epidemiology, School Veterinary Medicine, University California, Davis, CA 95616-8737. Stephen Dumler The Infectious Diseases, Volume...

SUMMARY Diagnosis of bacterial septicemia was confirmed by results bacteriologic culture antemortem blood samples and/or necropsy specimens obtained from 47 foals up to 8 days old. Gram-negative bacteria were isolated all foals, and mixed infections with more than one organism involved in 26 (55%). Escherichia coli , Actinobacillus spp, Klebsiella pneumoniae the most frequent isolates, infecting 55, 34, 23% respectively. Gram-positive anaerobic only gram-negative organisms. Clostridium...

ABSTRACT A TaqMan PCR was established for identification and quantitation of members the Ehrlichia phagocytophila group in experimentally infected cows Ixodes ricinus ticks. The identified a 106-bp section 16S rRNA gene by use specific fluorogenic probe two primers. This technique E. group, which include , equi agent human granulocytic ehrlichiosis. system 10 copies cloned . sensitivity specificity were similar to those conventional nested PCR. numbers ehrlichiae leukocytes with measured...

A Thoroughbred filly that developed clinical signs of equine granulocytic ehrlichiosis following inoculation with the human granulocytotropic ehrlichia was shown to be resistant challenge Ehrlichia equi, a closely related agent. This result further substantiates close and potentially conspecific relationship between these two ehrlichiae.

The prevalence of antibodies to Ehrlichia equi in horses from the foothill regions northern California and Sacramento valley (non-foothill area) was determined, using an indirect fluorescent antibody test. Horses had a higher seropositivity (10.4%) titer (1:10 1:80) than did those non-foothill (3.1%; less or equal 1:10). Fifty percent healthy on farm enzootic for E equi, suggesting that infection with can be subclinical. Six veterinarians surveyed diagnosed clinical 38 during 1985-1986 based...

Background Idiopathic headshaking ( HSK ) in horses is a distressing disorder which the etiology and pathophysiology are unknown. Hypothesis Differences sensory function of trigeminal nerve exist between healthy affected horses. Animals Six mature geldings 6 with idiopathic . Methods Prospective study. Sensory action somatosensory evoked potentials studies were performed. The stimulus site comprised gingival mucosa dorsal to maxillary canine. A pair recording electrodes was placed along...