A5046325260- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- RNA modifications and cancer
- Microtubule and mitosis dynamics
- RNA regulation and disease
- RNA Research and Splicing
- Viral Infectious Diseases and Gene Expression in Insects
- Gene Regulatory Network Analysis
- CRISPR and Genetic Engineering
- Enzyme Production and Characterization
- Food Industry and Aquatic Biology
- Genomics and Chromatin Dynamics
- Metabolism and Genetic Disorders
- 3D Printing in Biomedical Research
- Advanced Aircraft Design and Technologies
- Cancer-related Molecular Pathways
- Evolution and Genetic Dynamics
- Biofuel production and bioconversion
- Plant Molecular Biology Research
- RNA Interference and Gene Delivery
- Biochemical and Molecular Research
- Ruminant Nutrition and Digestive Physiology
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Chromosomal and Genetic Variations
Technical University of Denmark
2017
Novo Nordisk Foundation
2015-2017
University of California, Berkeley
2012-2017
Danish Geotechnical Society
2017
Foundation Center
2016
Energy Biosciences Institute
2013
Lawrence Berkeley National Laboratory
2013
University of Milano-Bicocca
2004-2009
Wellcome Centre for Cell Biology
2008-2009
University of Edinburgh
2008-2009
We have used a human artificial chromosome (HAC) to manipulate the epigenetic state of chromatin within an active kinetochore. The HAC has dimeric alpha-satellite repeat containing one natural monomer with CENP-B binding site, and completely synthetic box replaced by tetracycline operator (tetO). This exhibits normal kinetochore protein composition mitotic stability. Targeting several tet-repressor (tetR) fusions into centromere had no effect on function. However, altering more open...
Survivin is a key cellular protein thought to function in apoptotic regulation, mitotic progression, or possibly both. In this study, we describe the isolation of two conditional knockouts survivin gene chicken DT40 cells. cells lacking die interphase after failing complete cytokinesis. However, these show normal sensitivity chemotherapeutic agent etoposide. Expression mutants against null background reassess role several residues reveals that can grow normally if their sole missing widely...
We previously used a human artificial chromosome (HAC) with synthetic kinetochore that could be targeted chromatin modifiers fused to tetracycline repressor show targeting of the transcriptional tTS within disrupts structure and function. Here we corepressor KAP1, downstream effector tTS, can also inactivate kinetochore. The disruption by KAP1 subdomains does not simply result from loss centromeric CENP-A nucleosomes. Instead it reflects hierarchical outer kinetochore, CENP-C levels falling...
Mammalian cleavage factor I (CF m ) is an essential that required for the first step in pre-mRNA 3′ end processing. Here, we characterize CF 68 subnuclear distribution and mobility. Fluorescence microscopy reveals addition to paraspeckles accumulates structures partially overlap with nuclear speckles. Analysis of synchronized cells shows speckles varies during cell cycle. At ultrastructural level, associated perichromatin fibrils, sites active transcription, concentrates interchromatin...
Export of mRNA from the nucleus is linked to proper processing and packaging into ribonucleoprotein complexes. Although several observations indicate a coupling between 3' end formation export, it not known how these two processes are mechanistically connected. Here, we show that subunit mammalian pre-mRNA complex, CF I(m)68, stimulates export. I(m)68 shuttles cytoplasm in transcription-dependent manner interacts with export receptor NXF1/TAP. Consistent idea may act as novel adaptor for...
Production of chemicals in microbes often employs potent biosynthetic enzymes, which can interact with the microbial native metabolism to affect cell fitness and product yield. However, production optimization largely relies on data collected from wild-type strains absence metabolic perturbations, thus limiting their relevance specific conditions. Here, we address this issue by coupling thiamine diphosphate Escherichia coli using a synthetic RNA biosensor. We use strategy interrogate library...
We describe a method for the isolation of conditional knockouts essential multiply spliced genes in which entire body gene downstream ATG start codon is left untouched but can be switched off rapidly and completely by adding tetracycline to culture medium. The approach centers on "promoter-hijack" strategy gene's promoter replaced with minimal responsive tetracycline-repressible transactivator (tTA). Elsewhere genome, cloned fragment used drive expression tTA. Thus, essentially regulated its...
Cattle production is one of the key contributors to global warming due methane emission, which a by-product converting feed stuff into milk and meat for human consumption. Rumen hosts numerous microbial communities that are involved in digestive process, leading notable amounts emission. The factors underlying differences emission between individual animals to, among other factors, both specific enrichments certain host genetic influence abundances. detection such involves various...
The pursuit of standardization and reliability in synthetic biology has achieved, recent years, a number advances the design more predictable genetic parts for biological circuits. However, even with development high-throughput screening methods whole-cell models, it is still not possible to predict reliably how construct interacts all cellular endogenous systems. This study presents genome-wide analysis expression genes affected by systematic perturbations functions. We found that most...
Abstract The study presents an analysis of how different cellular functions link cell size to the expression synthetic genes in E. coli . Size-Expression interaction was mapped with a two-gene genetic probe across 3800 single-gene deletion strains. Through regression analysis, expression-specific effects and gene-specific were derived from generic effects, respectively. entire compendium broadly four systems distinct primary influence on map. Specifically, membrane structural components...
SUMMARY To produce chemicals, microbes typically employ potent biosynthetic enzymes that interact with native metabolism to affect cell fitness as well chemical production. However, production optimization largely relies on data collected from wild type strains in the absence of metabolic perturbations, thus limiting their relevance specific process scenarios. Here, we address this issue by coupling thiamine diphosphate Escherichia coli using a synthetic RNA biosensor. We apply system...