Estradiol-17β (E2) is a key hormone regulating reproductive functions in females. In pigs, E2, as the main conceptus signal, initiates processes resulting prolonged corpus luteum function, embryo development, and implantation. During early pregnancy endometrium undergoes morphological physiological transitions that are tightly related to transcriptome changes. Recently, however, importance of E2 primary signal pig has been questionable. Thus, aim present study was determine effects on...

Pregnancy establishment in mammals, including pigs, requires proper communication between embryos and the maternal reproductive tract. Prokineticin 1 (PROK1) has been described as a secretory protein with pleiotropic functions novel tissue-specific angiogenic factor. However, despite studies performed mainly on human cell lines mice, function of PROK1 endometrium during early pregnancy is still not fully elucidated. We hypothesized that contributes to pigs. The present study first report...

Abstract Acquisition of endometrial receptivity for embryo implantation is one the crucial processes during pregnancy and induced mainly by progesterone enhanced conceptus signals. Prokineticin 1 (PROK1) characterized as a secretory protein with diverse functions in various tissues, including reproductive tract. PROK1, its receptor PROKR1, are up-regulated porcine endometrium women’s receptive decidua. However, function PROK1 embryo-maternal communication has still not been fully elucidated....

Abstract Successful pregnancy establishment in mammals depends on proper embryo-maternal communication. Prokineticin 1 (PROK1) is a secretory protein that exerts pleiotropic functions various tissues. Despite the studies have primarily been performed with human cell lines and mice, function of PROK1 trophoblasts has still not fully elucidated. Hence, aim this study was to establish role during implantation placentation. mRNA elevated porcine early placentation period. Furthermore, we reveal...

Successful establishment and development of pregnancy requires proper communication between developing conceptuses the maternal reproductive tract. Prostaglandins are key players involved in regulation processes mammals including pigs. Due to its luteolytic action, prostaglandin F2-alpha (PGF2α) is mainly considered as an undesirable factor during early pregnancy. However, content uterine lumen elevated pigs other mammals. Recently, we reported important role PGF2α endometrium pig. Thus, aim...

Prokineticin 1 (PROK1) is an important factor in pregnancy establishment pigs, acting at the embryo-maternal interface and corpus luteum (CL). Estradiol-17β (E2) primary recognition signal its effects are augmented by luteotropic prostaglandin E2 (PGE2). On contrary, F2α (PGF2α) exerts mainly a luteolytic effect. The present study aimed to elucidate whether E2, PGE2, PGF2α regulate expression of PROK1 receptors porcine CL determine effect on luteal endothelial cells pathways that may be...

Successful pregnancy establishment in mammals depends on numerous interactions between embryos and the maternal organism. Estradiol-17β (E2) is primary embryonic signal pig, its importance has been questioned recently. However, E2 not only molecule of origin. In pigs, prostaglandin (PGE2) abundantly synthesized secreted by conceptuses endometrium. The present study aimed to determine role PGE2 simultaneous action with changes porcine endometrial transcriptome during establishment. effects...

The corpus luteum (CL) is a temporary endocrine gland vital for pregnancy establishment and maintenance. Estradiol-17β (E2) the major embryonic signal in pigs supporting CL’s function. mechanisms of luteoprotective action E2 are still unclear. present study aimed to determine effect on luteal expression factors involved CL An vivo model intrauterine infusions was applied. Gilts day 12 estrous cycle were used as referential groups. Concentrations progesterone elevated CLs gilts receiving...

Abstract Prokineticin 1 (PROK1) is a pleiotropic factor secreted by endocrine glands; however, its role has not been studied in the corpus luteum (CL) during pregnancy any species. The present study aimed to investigate contribution of PROK1 regulating processes related porcine CL function and regression: steroidogenesis, luteal cell apoptosis viability, angiogenesis. expression was greater on Days 12 14 compared Day 9. protein increased gradually peaked 14, when it also significantly higher...

Abstract During early pregnancy, porcine conceptuses (the embryos with associated membranes) secrete estradiol-17β (E2)—their major signal for maternal recognition of pregnancy—and prostaglandin E2 (PGE2). Both hormones induce prominent changes the endometrial transcriptome in vivo. Studies on pathologies have shown that affects gene expression by epigenetic mechanisms related to DNA methylation. Herein, we determined effects and PGE2 alone, a combined + treatment administered into uterine...

The objective of this study was to evaluate the kinetics bovine and porcine embryos that failed or developed blastocyst stage anomalies in cleavage using a time-lapse monitoring system. timing early cleavages their duration were similar for stage. There differences time first second cell division those did not develop (P=0.004 P=0.002), respectively. Similarly, case such difference observed only (P=0.0001). Direct from 1-cell 3 cells occurred 13.47% more than 3.37% whereas 3-cells 4.23%...

Objectives:The objective of this review is to provide synopsis on the latest technology and scientific achievements that have found application in reproductive medicine. Results:The a novel time-lapse into medicine promotes; 1) non-invasive observation early embryogenesis vitro provides an opportunity for more accurate understanding developmental dynamics embryo it's consequence further successful development, 2) establishment promising morphokinetic parameters developing identification...

This study aimed to examine the effect of vitrification on expression genes that are crucial for porcine early embryo development; cathepsin B (CTSB), growth differentiation factor 9 (GDF9), caudal type homeobox 2 (CDX2), and OCT-4, which play an important role in maintenance embryonic cell pluripotency. Their gene was investigated expanded blastocysts (day 6-7) derived from vitro matured oocytes. The quantitative real-time PCR method used assess amount relative specific transcripts 20...