Next-Generation Sequencing (NGS) methods specifically Whole-Exome (WES) have demonstrated promising findings with a high accuracy of 91%-99% in Pharmacogenomics (PGx). A PGx-based panel can be utilized to minimize adverse drug reactions (ADRs) and maximize the treatment efficacy. Remarkably, Cancer Pain Management (CPM) is cutting-edge concept modern medicine. Thus, this study aimed investigate WES results by containing genes involved Pain, Anti-inflammatory, Immunomodulating agents (PAIma)...

For many years, high-affinity subunit of IL-2 receptor (CD25) has been considered as a promising therapeutic target for different pathologic conditions like allograft rejection, autoimmunity, and cancers. Although CD25 is transiently expressed by newly-activated T cells, it the hallmark regulatory (Treg) cells which are most important immunosuppressive elements in tumor microenvironment. Thus, Tregs can be potential chimeric antigen (CAR)-based approaches. On other hand, due to some profound...

Parkinson’s disease (PD) is diagnosed when motor symptoms emerges, which almost 70% of dopamine neurons are lost. Therefore, early diagnosis PD crucial to prevent the progress disease. Blood-based biomarkers, minimally invasive, potentially used for PD, including miRNAs. The aim this study was assess whether SRRM2 and miR-27a/b-3p could act as diagnostic biomarkers PD. Total RNAs from PBMCs 30 PD’s patients 14 healthy age gender matched subjects extracted. expression levels respective genes...

A vast research has been conducted to find suitable and safe carriers for vital pH-sensitive drugs including antibiotics. This article reports the use of easily accessible abundant purified beta-lactoglobulin (β-LG) protein as potential carrier widely used Kanamycin (Kana) Ciprofloxacin (Cip) Spectroscopic techniques (Fluorescence, UV-vis, Circular Dichroism) combined with molecular docking were determine binding mechanism these drugs. Fluorescence studies showed moderate affinity calculated...

Abstract Non-enzymatic glycation of DNA and the associated effects are among pathogenic factors in diabetes mellitus. Natural polyphenols have anti-diabetic activity. Herein, protective role one phytochemicals, rosmarinic acid (RA), was evaluated (with fructose) human expression Akt genes hippocampus diabetic rats. In-vitro studies using fluorescence, agarose gel electrophoresis, fluorescence microscopy, thermal denaturation analyses revealed that causes damage RA inhibits it. In-vivo were...

Interstitial collagenas-1 degrades a variety of extracellular matrix components. A single Guanine insertion polymorphism in the promoter has been found that influences on transcription and expression level gene. It is suggested this may enhance susceptibility to some types cancer. Therefore, case-control study evaluated association genotype with initiation invasion colorectal For reason, whole blood samples were obtained from 150 CRC patients 100 control subjects Tehran. Genomic DNA was...

Background: The role of KDM3A and its downstream genes in male fertility has been approved animal models. Additionally, the expression shrinkage is significantly correlated with human azoospermia phenotype. Aberrant micro-RNAs could mislead spermatogenesis mostly lead to diverse phenotypes infertility. Objective: aim this study was evaluate level hsa-miR-27a- 3p azoospermic men reveal possible association Materials Methods: This case-control conducted on 30 men, whom, 19 had non obstructive...

β-thalassemia is the most common monogenic disorder in human. The (C-->T) polymorphism at -158 upstream region of γG-globin gene and pharmacological factors such as hydroxyurea have been reported to influence γ-globin expression severity clinical symptoms β-thalassemia.In present study, 51 intermediate patients were studied. Xmn1γG genotype was determined using Tetra-Primer ARMS-PCR technique. Hemoglobin (Hb) fetal hemoglobin (HbF) levels by gel electrophoresis.Of patients, 35 (68.6%)...

HO-1 gene encodes heme oxygenase-1 enzyme that catalyzes the oxidation of to carbon monoxide (CO). It has also been suggested cells could be protected by against stress. A (GT) n dinucleotide repeat at promoter is a polymorphic region and modulates transcription associated with some diseases. In this study, length polymorphism GT tandem determined classified into two alleles short (≤28) long (≥29). present association between GT-repeat increased risk gastric cancer metastasis was...

Background: Some previous human and animal studies have supported the idea that KDM3A down-regulation might be main cause of male infertility, especially in nonobstructive azoospermia (NOA). The regulatory role micro-RNAs (miRNA) has been investigated development infertility.
 Objective: expression level hsa-miR-30a-5p was evaluated to reveal its possible association with etiology Materials Methods: In this case-control study, 30 men (19 whom had NOA) were selected as case individuals,...

The genetic association between cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations and male infertility due to congenital bilateral absence of vas deferens (CBAVD) is well established. Mutant CFTR, however may also be involved in the etiology non-CBAVD cases. present study was conducted estimate frequency ∆I507 ∆F508 CFTR Iranian infertile males. We undertook first these non-obstructive azoospermia Iran. In this case-control study, 100 fertile healthy fathers...

A nanodiagnostic genotyping method was presented for point mutation detection directly in human genomic DNA based on ligase reaction coupled with quantum dots and magnetic nanoparticle-based probes.

Background: Lung cancer is considered as one of the most frequent cancers worldwide, and has been cause more than million mortalities each year. Exposure to tobacco smoke primary lung cancers, since it contains several thousand compounds, including 50 known carcinogens. However, a small fraction individuals who are exposed develop cancer, therefore genetic factors may render some smokers susceptible cancer. Objectives: Genetic polymorphism in genes that encode metabolizing enzymes be related...