A5004472404- Circadian rhythm and melatonin
- Photoreceptor and optogenetics research
- Zebrafish Biomedical Research Applications
- Genetics, Aging, and Longevity in Model Organisms
- Neurobiology and Insect Physiology Research
- Animal Behavior and Reproduction
- Spaceflight effects on biology
- Fish Ecology and Management Studies
- Marine Ecology and Invasive Species
- Physiological and biochemical adaptations
- Subterranean biodiversity and taxonomy
- Light effects on plants
- Marine and fisheries research
- Retinal Development and Disorders
- Birth, Development, and Health
- Climate Change and Health Impacts
- Air Quality and Health Impacts
- Morphological variations and asymmetry
- Reproductive biology and impacts on aquatic species
- Neurogenesis and neuroplasticity mechanisms
- Protist diversity and phylogeny
- Olfactory and Sensory Function Studies
- Hemispheric Asymmetry in Neuroscience
- Sleep and related disorders
- Marine Biology and Ecology Research
University of Ferrara
2015-2025
The circadian clock is synchronized with the day-night cycle primarily by light. Fish represent fascinating models for deciphering light input pathway to vertebrate since fish cell clocks are regulated direct exposure. Here we have performed a comparative, functional analysis of involving zebrafish that normally exposed and cavefish species has evolved in perpetual darkness. Our results reveal retains food-entrainable oscillates an infradian period. Importantly, however, this not This...
Abstract Cerebral lateralisation, the differential cognitive processing in two brain hemispheres, is variable among individuals most vertebrates. Part of this variance has been attributed to plasticity response environmental stressors experienced by and might be therefore mediated action glucocorticoids (GCs). Accordingly, we tested hypothesis that GC pathway related stress, which involved its cognate receptor GR, affects individuals’ lateralisation. First, characterised behavioural...
Daily light and feeding cycles act as powerful synchronizers of circadian rhythmicity. Ultimately, these external cues entrain the expression clock genes, which generate daily rhythmic behavioral physiological responses in vertebrates. In present study, we investigated genes a marine teleost (gilthead sea bream). Partial cDNA sequences key elements from both positive (Bmal1, Clock) negative (Per2, Cry1) regulatory loops were cloned before studying how time affects rhythms locomotor activity...
Objective— Diurnal variations in levels of factor VII (FVII), FVIII, proteins C and S, antithrombin, plasminogen activator inhibitor-1, prothrombin fragment F 1+2 , D-dimers healthy humans point to the existence circadian rhythms coagulation factors. We sought for temporal fluctuations tissue pathway inhibitor (TFPI) activity human mouse plasma. Methods Results— TFPI showed significant daily with highest morning men (+11%) mice at light-to-dark transition (+63%), beginning physically active...
Light plays a key role in synchronizing rhythms and setting the phase of early development. However, to date, little is known about impact light wavelengths during ontogeny molecular clock behavioural rhythmicity. The aim this research was determine effect different (white, blue red) on onset locomotor activity gene (per1b, per2, clock1, bmal1 dbp) expression rhythms. For purpose, 4 groups zebrafish embryo/larvae were raised from 0 7 days post-fertilization (dpf) under following lighting...
Brain-derived neurotrophic factor (BDNF) plays a pivotal role in neuronal growth and differentiation, plasticity, learning, memory. Using CRISPR/Cas9 technology, we generated vital Bdnf null mutant line zebrafish carried out its molecular behavioral characterization. Although no defects are evident on morphological inspection, 66% of coding genes 37% microRNAs turned to be differentially expressed bdnf-/- compared with wild type sibling embryos. We deeply investigated the circadian clock...
Abstract The circadian clock is a highly conserved cell-autonomous mechanism that directs daily rhythms in most aspects of biology. Daily entrainment by environmental signals, notably light, essential for its function. However, our understanding the mechanisms and evolution photic remains incomplete. Fish represent attractive models exploring how light regulates due to direct sensitivity their peripheral clocks. Central this property induced expression genes mediated D-box enhancer elements....
Light represents the principal signal driving circadian clock entrainment. However, how light influences evolution of remains poorly understood. The cavefish Phreatichthys andruzzii a fascinating model to explore under extreme aphotic conditions shapes clock, since in this species is unresponsive light. We have previously demonstrated that loss-of-function mutations targeting non-visual opsins contribute part blind phenotype. Here, we compared orthologs two core genes play key role photic...
Glucocorticoids (GCs) are steroid hormones mainly acting as key regulators of body homeostasis and stress responses. Their activities primarily based on the binding to GC receptor (GR), a member nuclear family, that regulates tissue-specific sets genes. GCs secretion follows circadian rhythmicity with peak linked animal’s activity phase. In mammals, also implicated in feeding entrainment mechanisms internal zeitgeber. Here, we investigated, by means behavioural molecular approaches, clock...
Most passerine birds are nocturnal migrants. When kept in captivity during the migratory periods, these species show a restlessness, or Zugunruhe. Recent studies on Sylvia warblers have shown that Zugunruhe is an excellent proxy of disposition. Passerine can use Earth's geomagnetic field as compass to keep their course flight. Among candidate magnetoreceptive mechanisms cryptochromes, flavoproteins located retina supposed perceive magnetic through light-mediated process. Previous work has...
Fish present daily and seasonal rhythms in spawning plasmatic levels of steroids that control reproduction. However, the existence expression genes underlie endocrine mechanisms responsible for processes such as steroidogenesis reproduction fish have still been poorly explored to date. Here we investigated pattern key involved sex steroid production ultimately set ratio fish. Adult zebrafish were maintained under a 12:12 h light-dark cycle at constant temperature 27°C sampled every 4 during...
The remarkable similarities in cognitive performance between teleosts and mammals suggest that the underlying mechanisms might also be similar these two groups. We tested this hypothesis by assessing effects of brain-derived neurotrophic factor (BDNF), which is critical for mammalian functioning, on fish's abilities. found individual differences zebrafish's learning abilities were positively correlated with bdnf expression. Moreover, a CRISPR/Cas9 mutant zebrafish line lacks BDNF gene...
Many physiological and behavioural responses to changes in environmental lighting conditions are mediated by extraocular photoreceptors. Here we investigate encephalic photoreception Phreatichthys andruzzii, a typical cave-dwelling fish showing an extreme phenotype with complete anophthalmy reduction size of associated brain structures. We firstly identified two P. andruzzii photopigments, orthologues rod opsin exo-rod opsin. In vitro, both opsins serve as light-absorbing photopigments...
Distinguishing familiar from novel stimuli is critical in many animals' activities, and procedures based on this ability are among the most exploited translational research rodents. However, recognition learning underlying brain substrates remain unclear outside a few mammalian species. Here, we investigated one-trial for olfactory teleost fish using behavioural molecular approach. With our analysis, found that zebrafish can learn to recognise odour after single encounter then, discriminate...
The molecular mechanisms whereby the circadian clock responds to temperature changes are poorly understood. ruin lizard Podarcis sicula has historically proven be a valuable vertebrate model for exploring influence of on physiology. It is an ectotherm that naturally experiences impressive range temperatures during course year. However, no tools have been available dissect basis in this organism. Here, we report cloning three gene homologs (Period2, Cryptochrome1, and Clock) close...