A5056926256- 3D Printing in Biomedical Research
- Liver physiology and pathology
- Pluripotent Stem Cells Research
- Drug-Induced Hepatotoxicity and Protection
- Biomedical Ethics and Regulation
- Pharmacogenetics and Drug Metabolism
- Alcohol Consumption and Health Effects
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Receptor Mechanisms and Signaling
- Extracellular vesicles in disease
- Cellular Mechanics and Interactions
- Drug Transport and Resistance Mechanisms
- Animal testing and alternatives
- Periodontal Regeneration and Treatments
- Erythrocyte Function and Pathophysiology
- Wound Healing and Treatments
- Reproductive System and Pregnancy
- Liver Disease Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Pharmaceutical studies and practices
- Pancreatic function and diabetes
- T-cell and B-cell Immunology
- Metabolism and Genetic Disorders
- CRISPR and Genetic Engineering
University of Helsinki
2024
Aalto University
2024
University of Liverpool
2003-2023
Karolinska Institutet
2021-2022
Platelet-rich plasma (PRP) is a source of growth factors, which are implicated in active tissue regeneration. However, after transplantation the efficacy these bioactive compounds often diminished due to rapid degradation and untargeted localization. For this reason, we evaluated potential nanofibrillated cellulose (NFC) hydrogel as PRP carrier. NFC an animal-free biomaterial that, when doped with cellulase, can assist release wound site. In study, examined effects 0.5% (m/v) formulations,...
Background. Natural killer (NK) cells play an important role in several animal models of autoimmunity by modulating T‐cell responses, but it is unclear whether human NK have similar functions.
Emerging hepatic models for the study of drug-induced toxicity include pluripotent stem cell-derived hepatocyte-like cells (HLCs) and complex hepatocyte-non-parenchymal cellular coculture to mimic multicellular interactions that recapitulate niche environment in human liver. However, a specific marker hepatocyte perturbation, required discriminate damage from non-specific contributed by non-hepatocyte cell types or immature differentiated is currently lacking, as cytotoxicity assays...
The recent FDA decision to eliminate animal testing requirements emphasises the role of cell models, such as spheroids, regulatory test alternatives for investigations cellular behaviour, drug responses, and disease modelling. influence environment on spheroid formation are incompletely understood, leading uncertainty in matrix selection scaffold-based 3D culture. This study uses atomic force microscopy-based techniques quantify adhesion Matrigel cellulose nanofibrils (CNF), cell-cell...
Variations in oxygen level affect the phenotype of cells and extracellular vesicles (EVs). Depending on metabolic demand cells, hypoxic cell culture can produce conditions more like those found vivo, with appropriate levels, mimic tumours. However, most previous experiments studying both EVs effects hypoxia use periods 72 h or less hypoxia. We hypothesised that this was insufficient time for adaptation to which may skew results such studies. In study, acute (72 h) chronic (> 2 weeks) HepG2...
During pharmacotherapy, knowledge about the actual drug and metabolite concentrations in plasma is often critical. Individual dose adjustments can be performed based on pre-emptive genotyping of certain absorption, distribution, metabolism, excretion (ADME) genes but also using therapeutic monitoring (TDM). Analyses liquid biopsies for tumor-derived components are well-established have been found to a good complement biopsy examinations. Recently, biopsy-based quantification cell-free RNA...
Human pluripotent stem cells (hPSCs) are a promising source of somatic for clinical applications and disease modelling. However, during culture they accumulate genetic aberrations such as amplification 20q11.21 which occurs in approximately 20% extensively cultured hPSC lines confers BCL2L1-mediated survival advantage. During the production large number required transplantation therapy these may become unavoidable has important safety implications therapies also impact upon Presently, risks...
The in vivo-relevant phenotype of 3D liver spheroids allows for long-term studies of, e.g., novel mechanisms chronic drug-induced toxicity. Using this system, we present a stress response human and murine hepatocyte spheroids, wherein long slender filaments form after treatment with four different drugs, which three are PPARα antagonists. morphology the thorns varies between donors compounds used. They mainly composed diverse protein fibres, glycosylated. Their formation is inhibited by...
Drug-induced liver injury (DILI) remains a burden to the public, pharmaceutical industry and regulators. Not only do we generally lack clear mechanisms that can explain injury, but manifestations of DILI are diverse, they difficult diagnose, also take weeks or even months develop. Whilst there few good animal models DILI, similarly in vitro at present. Therefore, believe it is important understand what our vitro/in vivo fit for as well their limitations. Otherwise, model development will not...