A5019253684- Bone Metabolism and Diseases
- Bone health and treatments
- Bone and Dental Protein Studies
- Oral microbiology and periodontitis research
- Inflammatory mediators and NSAID effects
- Immune Response and Inflammation
- Dental Health and Care Utilization
- Protease and Inhibitor Mechanisms
- NF-κB Signaling Pathways
- Liver Disease Diagnosis and Treatment
- Endodontics and Root Canal Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Food Science and Nutritional Studies
- Advanced Glycation End Products research
- Biomedical Ethics and Regulation
- Neuropeptides and Animal Physiology
- Atrial Fibrillation Management and Outcomes
- Hemoglobin structure and function
- Cytokine Signaling Pathways and Interactions
- Periodontal Regeneration and Treatments
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Synthesis and bioactivity of alkaloids
- Pharmacological Effects of Medicinal Plants
- Oral and Craniofacial Lesions
- Kruppel-like factors research
Nihon University
2013-2024
Morpho (United States)
2019-2024
Hagoromo International University
2018
RIKEN Center for Integrative Medical Sciences
2012
RIKEN
2012
Surugadai Nihon University Hospital
2012
Showa University Fujigaoka Hospital
1987
Tobacco smoking is an important risk factor for the development of several cancers, osteoporosis, and inflammatory diseases such as periodontitis. Nicotine one major components tobacco. In previous study, we showed that nicotine inhibits mineralized nodule formation by osteoblasts, culture medium from osteoblasts containing lipopolysaccharide increases osteoclast differentiation. However, direct effect on differentiation function osteoclasts poorly understood. Thus, examined effects...
Epidemiological studies have reported that periodontitis and cardiometabolic disease such as cardiovascular type 2 diabetes are associated; however, there been very few prospective cohort on this topic. Therefore, we conducted a 9-year follow-up study to examine the relationship between duration of risk factors, including hypertension, hyperglycemia, dyslipidemia, obesity.The participants comprised 572 adult industrial workers (417 men 155 women; mean age, 37.4 years) who had undergone...
Angiotensin II (Ang II) not only regulates systemic blood pressure through a vasoconstrictive effect, but also promotes bone resorption. We recently reported that Ang (10(-6) M) stimulated the production of matrix metalloproteinases via AT1 receptor in osteoblastic ROS17/2.8 cells, suppressed alkaline phosphatase activity. However, roles differentiation and function osteogenesis osteoblasts are unclear. Therefore, we examined effect on expression osteogenesis-related transcription factors...
Diabetes is a metabolic disorder that causes long-term hyperglycemic state with complications affect multiple tissues, including bone. HMGB1, nonhistone chromosomal binding protein, released from the nucleus of damaged cells and secreted extracellularly, where it mediates inflammatory responses. Hyperglycemia induces HMGB1 expression in cell types associated diabetic complications. Therefore, we evaluated effect high glucose concentration on production MG-63 osteoblast-like cells. were...
BACKGROUND:Osteoclast precursor cells are constitutively differentiated into mature osteoclasts on bone tissues. We previously reported that the continuous stimulation of RAW264.7 with compressive force induces formation multinucleated giant via receptor activator nuclear factor κB (RANK)-RANK ligand (RANKL) signaling. Here, we examined resorptive function induced by force. MATERIAL AND METHODS:Cells were continuously stimulated 0.3, 0.6, and 1.1 g/cm² created increasing amount culture...
The association between obesity and inflammation is well documented in epidemiological studies.Proteolysis of extracellular matrix (ECM) proteins involved adipose tissue enlargement, metalloproteinases (MMPs) collectively cleave all ECM proteins.Here, we examined the effects C-reactive protein (CRP), an inflammatory biomarker, on expression MMPs inhibitors (TIMPs), which are natural MMPs, adipocyte-differentiated 3T3-L1 cells.We analyzed Fcγ receptor (FcγR) IIb FcγRIII, candidates for CRP...
This study examined the effects of continual Gram-negative bacterial challenge on stroke onset. Stroke onset occurred significantly earlier in stroke-prone spontaneously hypertensive rats (SHRSP) injected with a cell suspension rods or lipopolysaccharides (LPSs) than uninjected controls. Paralysis hindlimb, piloerection, hypokinesis, and hyperkinesis were observed LPS-injected SHRSP but not controls during The serum levels NOx, thiobarbituric acid reactive substance, 8-hydroxydeoxyguanosine...
Inflammation-mediated bone loss is a major feature of various diseases, including chronic periodontitis, rheumatoid arthritis, and osteoarthritis, due to an imbalance in remodeling that favors resorption. This caused by increased cytokine levels the inflamed tissue. Interleukin (IL)-17 secreted primarily activated Th17 cells, IL-17s IL-17 receptors play important role many autoimmune inflammatory diseases. The aim this paper review differences between indirect effect IL-17A via osteoblasts...
BACKGROUND The interplay between obesity and periodontitis has been widely examined. While was reported as a risk factor for periodontitis, the inverse relationship is still little explored. Therefore, we aimed to determine whether toothbrushing frequency affect onset of obesity. MATERIAL AND METHODS This cohort study included 1619 employees business enterprise headquartered in Tokyo, who 2002 2006 underwent prescribed annual health checks, both general dental-specific, were not obese (body...
Objective: Matrix metalloproteinases (MMPs), produced by osteoblasts, catalyze the turnover of extracellular matrix (ECM) molecules in osteoid, and regulation MMP activity depends on interactions between MMPs tissue inhibitors (TIMPs).We focused degradation process ECM osteoid that was exposed to mechanical strain, conducted an vitro study using MC3T3-E1 osteoblastic cells examine effects tension force (TF) expression TIMPs, activation mitogen-activated protein kinase (MAPK) pathways.Design:...
Interleukin (IL)-17 plays an important role in many autoimmune and inflammatory diseases. We recently demonstrated the various direct indirect effects of IL-17A on osteoclastogenesis. However, effect bone matrix formation by osteoblasts is unclear. Therefore, we examined IL-17 receptor (IL-17R), extracellular protein (ECMP), cyclooxygenase (COX) expression; prostaglandin (PG) E2 production; alkaline phosphatase (ALPase) activity; mineralized nodule osteoblastic ROS17/2.8 cells. also PGE2...
The receptor activator of NF-κB (RANK) ligand (RANKL) is a cytokine that essential for osteoclast development, whereas interleukin (IL)-18 suppresses osteoclastogenesis by increasing granulocyte-macrophage colony-stimulating factor (GM-CSF) production in T-cells. In the present study, we examined effect RANKL on expression IL-18 and binding protein (IL-18BP), natural inhibitor IL-18, RAW264.7 cells. We also conditioned medium derived from cells IL-18-induced GM-CSF CD4+ T isolated spleens...
In the present study, experimental periodontitis was induced by ligature in Wistar rats that were not on high-fat or high-carbohydrate diet. The metabolic and inflammatory parameters serum examined order to assess effect of periodontitis-induced inflammation syndrome individual without genetic dietary risk factors. Ligature-induced increased monocyte chemoattractant protein-1 triglyceride levels serum, as well homeostasis model assessment insulin resistance, based concentration glucose rats....
Diet is an important factor maintaining bone health. Several studies have reported that extracts of brown marine algae containing polyphenols positively regulate metabolism. Therefore, we examined the effects Eisenia bicyclis (Japanese name, Arame), edible algae, on cell proliferation, alkaline phosphatase activity, collagenous and non-collagenous matrix protein expressions, mineralized nodule formation using ROS17/2.8 cells as osteoblasts. A thermal extract derived from E. was applied to a...
Electric-toothbrush vibrations, which remove plaque, are transmitted to the gingival connective tissue via epithelial cells. Physical energy affects cell function; however, effects of electric-toothbrush vibrations on extracellular matrix (ECM) protein expression remain unknown. We aimed examine these ECM proteins—type I collagen (col I), type III III), elastin, and fibronectin (FN)—using human fibroblasts (HGnFs). HGnFs were seeded for 5 days in a six-well plate with hydrophilic surface,...
Propofol affects the central nervous system rapidly and works as an anesthetic. After intravenous administration, cells resident in bone tissues are also exposed to propofol. Osteoclasts can form actin rings on surfaces express carbonic anhydrase II (Car2), cathepsin K (Ctsk), matrix metalloproteinase-9 (Mmp9), which enzymes associated with decomposition play a role resorption. This study examined expression of involved resorption ring formation stimulated by propofol elucidate difference...
Osteoclasts are associated with normal bone remodeling in healthy tissues and excess resorption bone-destructive diseases. Bidens pilosa is a plant belonging to the Asteraceae family well known for its antioxidative stress effect. In present study, RAW264.7 cells were treated receptor activator of NF-kappaB ligand (RANKL), which predominant foci pathological resorption, or without extracts B. harvested Miyako Island, Japan (MBP) examine effects MBP on osteoclastogenesis. Osteoclast...
Sodium fluoride (NaF) is a application recommended by the World Health Organization for its efficacy and safety in preventing dental caries. Gingival fibroblasts that constitute majority of connective tissue cells play major role wound healing via expression growth factors, including fibroblast factor-2 (FGF-2) transforming factor-beta (TGF-β). This study examined effect NaF mouthwash on FGF-2 TGF-β human gingival (HGnFs). Fibroblasts were exposed to medium with 225 ppmF 1 min, then switched...
【目的】オカラを食用微生物であるテンペ菌で発酵させた発酵オカラ（OT）にバナナを加えたオカラ発酵素材（OTB）を開発した。本研究ではヒトにおいて血糖とインスリンに対するOTB粉末の影響を探索的に検討した。