A5070546835- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Insect Resistance and Genetics
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Developmental Biology and Gene Regulation
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Geological Studies and Exploration
- Genetic diversity and population structure
Institute of Gene Biology
2013-2024
Russian Academy of Sciences
2014
The emergence of whole-genome assays has initiated numerous genome-wide studies transcription factor localizations at genomic regulatory elements (enhancers, promoters, silencers, and insulators), as well facilitated the uncovering some key principles chromosomal organization. However, proteins involved in formation maintenance architecture organization domains remain insufficiently studied. This review attempts to collate available data on abundant but still poorly understood family with...
Insulators are multiprotein–DNA complexes that regulate the nuclear architecture. The Drosophila CP190 protein is a cofactor for DNA-binding insulator proteins Su(Hw), CTCF, and BEAF-32. fact has been found at genomic sites devoid of either known factors until now unexplained. We have identified two zinc-finger proteins, Pita, new factor named ZIPIC, interact with in vivo vitro specific interaction domains. Genomic binding these clustered as well CTCF Model Pita or ZIPIC demonstrate partial...
According to recent models, as yet poorly studied architectural proteins appear be required for local regulation of enhancer-promoter interactions, well global chromosome organization. Transcription factors ZIPIC, Pita and Zw5 belong the class chromatin insulator preferentially bind promoters near TSS extensively colocalize with cohesin condensin complexes. are structurally similar in containing N-terminal zinc finger-associated domain (ZAD) different numbers C2H2-type fingers at C-terminus....
Abstract In arthropods, zinc finger-associated domains (ZADs) are found at the N-termini of many DNA-binding proteins with tandem arrays Cys2-His2 fingers (ZAD-C2H2 proteins). ZAD-C2H2 undergo fast evolutionary lineage-specific expansion and functional diversification. Here, we show that all ZADs from Drosophila melanogaster form homodimers, but only certain high homology can also heterodimerize. CG2712, for example, is unable to heterodimerize its paralog, previously characterized insulator...
The binding of the Drosophila male-specific lethal dosage compensation complex (DCC) exclusively to male X chromosome provides an excellent model system understand mechanisms selective recruitment protein complexes chromatin. Previous studies showed that organizer complex, MSL2, and ubiquitous DNA-binding CLAMP are key players in specificity binding. CXC domain MSL2 binds genomic sites DCC vitro. Another conserved named Clamp-binding (CBD) directly interacts with N-terminal zinc finger...
Boundaries in the Drosophila bithorax complex delimit autonomous regulatory domains that activate parasegment (PS)-specific expression of homeotic genes. The Fab-7 boundary separates iab-6 and iab-7 control Abd-B PS11 PS12. This is composed multiple functionally redundant elements has two key activities: it blocks crosstalk between facilitates bypass. Here, we have used a structure–function approach to elucidate biochemical properties vivo activities conserved BEN domain protein,...
Abstract Boundaries (insulators) in the Drosophila bithorax complex (BX-C) delimit autonomous regulatory domains that orchestrate parasegment (PS)-specific expression of BX-C homeotic genes. The Fab-7 boundary separates iab-6 and iab-7 domains, which control Abd-B PS11 PS12, respectively. This is composed multiple functionally redundant elements has two key functions: it blocks cross talk between facilitates bypass. Here, we show BEN domain protein complexes, Insensitive Elba, bind to...
Abstract In Drosophila, a group of zinc finger architectural proteins recruits the CP190 protein to chromatin, an interaction that is essential for functional activity promoters and insulators. this study, we describe new C2H2 called Madf Zinc-Finger Protein 1 (Mzfp1) interacts with CP190. Mzfp1 has unusual structure includes six domains organized in C-terminal cluster two tandem MADF domains. predominantly binds housekeeping gene located both euchromatin heterochromatin genome regions. vivo...
Insulators are defined as a class of regulatory elements that delimit independent transcriptional domains within eukaryotic genomes. The first insulators to be identified were scs and scs', which flank the domain including two heat shock 70 genes. Zw5 BEAF bind respectively, responsible for interaction between these insulators. Using regions yellow white reporter genes, we have found scs' improves enhancer-blocking activity weak insulator. sequences include promoters genes strongly active in...
Abstract The binding of Drosophila male-specific lethal (MSL) dosage compensation complex exclusively to male X chromosome provides an excellent model system understand mechanisms selective recruitment protein complexes chromatin. Previous studies showed that the organizer complex, MSL2, and ubiquitous DNA-binding CLAMP are key players in specificity binding. CXC domain MSL2 binds genomic sites MSL recruitment. Here we demonstrated directly interacts with N-terminal zinc-finger CLAMP....
ABSTRACT The binding of the Drosophila male-specific lethal dosage compensation complex (DCC) exclusively to male X chromosome provides an excellent model system understand mechanisms selective recruitment protein complexes chromatin. Previous studies showed that organizer complex, MSL2, and ubiquitous DNA-binding CLAMP are key players in specificity binding. CXC domain MSL2 binds genomic sites DCC vitro. Another conserved named Clamp-binding (CBD) directly interacts with N-terminal...