A5019123020- Computational Drug Discovery Methods
- Animal testing and alternatives
- Effects and risks of endocrine disrupting chemicals
- Spectroscopy and Chemometric Analyses
- Metabolomics and Mass Spectrometry Studies
- Environmental Toxicology and Ecotoxicology
- Pesticide Residue Analysis and Safety
- Gene expression and cancer classification
- Statistical Methods in Clinical Trials
- Carcinogens and Genotoxicity Assessment
- Analytical Chemistry and Chromatography
- Hormonal and reproductive studies
- Estrogen and related hormone effects
- Pharmaceutical and Antibiotic Environmental Impacts
- Molecular Biology Techniques and Applications
- Immunotoxicology and immune responses
- Chemistry and Chemical Engineering
- Advanced Chemical Sensor Technologies
- Agricultural safety and regulations
- Biomedical Text Mining and Ontologies
- Insect Utilization and Effects
- Air Quality and Health Impacts
- Agriculture Sustainability and Environmental Impact
- Dye analysis and toxicity
- Pesticide Exposure and Toxicity
Syngenta (United States)
2024
Integrated Laboratory Systems, Inc.
2017-2022
Research Triangle Park Foundation
2016-2022
Triangle
2017-2021
Environmental Protection Agency
2016-2018
Oak Ridge Associated Universities
2017
Oak Ridge Institute for Science and Education
2017
Michigan State University
2015
The median lethal dose for rodent oral acute toxicity (LD50) is a standard piece of information required to categorize chemicals in terms the potential hazard posed human health after exposure. exclusive use vivo testing limited by time and costs performing experiments need sacrifice number animals. (Quantitative) structure-activity relationships [(Q)SAR] proved valid alternative reduce assist assays assessing toxicological hazard. In framework new international collaborative project, NTP...
Changes in gene expression can help reveal the mechanisms of disease processes and mode action for toxicities adverse effects on cellular responses induced by exposures to chemicals, drugs environment agents. The U.S. Tox21 Federal collaboration, which currently quantifies biological nearly 10,000 chemicals via quantitative high-throughput screening(qHTS) vitro model systems, is now making an effort incorporate profiling into existing battery assays. Whole transcriptome analyses performed...
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In early 2018, the Interagency Coordinating Committee for Validation of Alternative Methods (ICCVAM) published "Strategic Roadmap Establishing New Approaches to Evaluate Safety Chemicals and Medical Products in United States" (ICCVAM 2018). Cross-agency federal workgroups have been established implement this roadmap various toxicological testing endpoints, with an initial focus on acute toxicity testing. The ICCVAM workgroup (ATWG) helped organize a global collaboration build predictive...
Background: Low-cost, high-throughput in vitro bioassays have potential as alternatives to animal models for toxicity testing. However, incorporating into chemical evaluations such read-across requires significant data curation and analysis based on knowledge of relevant mechanisms, lowering the enthusiasm using massive amount unstructured public data. Objective: We aimed develop a computational method automatically extract useful bioassay from repository (i.e., PubChem) assess its ability...
Thousands of chemicals are directly added to or come in contact with food, many which have undergone little no toxicological evaluation. The landscape the food-relevant chemical universe was evaluated using cheminformatics, and subsequently bioactivity across publicly available ToxCast highthroughput screening program assessed. In total, 8659 were compiled including direct food additives, substances, pesticides. Of these chemicals, 4719 had curated structure definition files amenable...
New approach methodologies (NAMs) for toxicological applications such as in vitro assays and silico models generate data that can be useful assessing potential health impacts of chemicals. The National Toxicology Program's (NTP's) Integrated Chemical Environment (ICE; https://ice.ntp.niehs.nih.gov/) provides user-friendly access to NAM tools explore contextualize chemical bioactivity molecular properties. ICE contains curated vivo toxicity testing experimental physicochemical property...
Abstract Regulatory agencies rely upon rodent in vivo acute oral toxicity data to determine hazard categorization, require appropriate precautionary labeling, and perform quantitative risk assessments. As the field of toxicology moves toward animal-free new approach methodologies (NAMs), there is a pressing need develop reliable, robust reference set characterize reproducibility inherent variability test method, which would serve contextualize results expectations regarding NAM performance....
Disruption of steroidogenesis by environmental chemicals can result in altered hormone levels causing adverse reproductive and developmental effects. A high-throughput assay using H295R human adrenocortical carcinoma cells was used to evaluate the effect 2060 chemical samples on via high-performance liquid chromatography followed tandem mass spectrometry quantification 10 steroid hormones, including progestagens, glucocorticoids, androgens, estrogens. The study employed a 3 stage screening...
The U.S. Environmental Protection Agency Endocrine Disruptor Screening Program and the Organization for Economic Co-operation Development (OECD) have used human adrenocarcinoma (H295R) cell-based assay to predict chemical perturbation of androgen estrogen production. Recently, a high-throughput H295R (HT-H295R) was developed as part ToxCast program that includes measurement 11 hormones, including progestagens, corticosteroids, androgens, estrogens. To date, 2012 chemicals been screened at 1...
In toxicology and regulatory testing, the use of animal methods has been both a cornerstone subject intense debate. To continue this discourse panel audience representing scientists from various sectors countries convened at workshop held during 12th World Congress on Alternatives Animal Use in Life Sciences (WC-12). The ensuing discussion focused scientific ethical considerations surrounding necessity responsibility defending creation new data testing. primary aim was to foster an open...
Packaging is an indispensable component of the food manufacturing and supply process. This scientific workshop was convened to bring together scientists from government, academia, industry discuss state science regarding safety packaging, prompted by rapidly advancing research improve packaging that continues impact technology, toxicology, exposure, risk assessment, sustainability. The opening session focused on challenges in assessment materials. Experts discussed migration contaminant...
Humans are exposed to large numbers of chemicals during their daily activities. To assess and understand potential health impacts chemical exposure, investigators regulators need access reliable toxicity data. In particular, data for a wide range chemistries needed support development new approach methodologies (NAMs) such as computational models, which offer increased throughput relative traditional approaches reduce or replace animal use. NAMs evaluation require chemically diverse sets...
Programs including the ToxCast project have generated large amounts of in vitro high‒throughput screening (HTS) data, and best approaches for interpretation use HTS chemical safety assessment, remain to be evaluated. To fill this gap, we conducted case studies two indirect food additive chemicals where data were compared with vivo toxicity using RISK21 approach. Two contact substances, sodium (2-pyridylthio)-N-oxide dibutyltin dichloride, selected, available exposure model predictions...
In the real world, individuals are exposed to chemicals from sources that vary over space and time. However, traditional risk assessments based on in vivo animal studies typically use a chemical-by-chemical approach apical disease endpoints. New methodologies (NAMs) toxicology, such as vitro high-throughput (HTS) assays generated Tox21 ToxCast, can more readily provide mechanistic chemical hazard information for with no existing data than methods. this paper, we establish workflow assess...
Atrazine (ATR) is a broad-spectrum triazine herbicide that disrupts steroidogenesis resulting in reproductive and developmental toxicity at high doses. Mouse BLTK1 Leydig cells were used as steroidogenic model to investigate the effects of ATR on testosterone (T) biosynthesis. Induction by 3 ng/ml recombinant human chorionic gonadotropin (rhCG) induced intracellular 3′,5′ cyclic adenosine monophosphate (cAMP) approximately 20-fold T 3-fold 4 h. Co-treatment with 300 μM super-induced cAMP...
Recent years have been marked by an increased interest in alternative proteins as food sources. Protein choices are no longer limited to traditional plant and animal proteins; rather, there is a growing from novel approaches applied various plants, insects, cell-cultured or fermented Global trade economic growth fueling shifts nutritional preferences new consumer demands placing pressure on supply chains demanding greater transparency about what they eat. As this trend gains momentum,...
Rapidly evolving technological methods and mechanistic toxicological understanding have paved the way for new science-based approaches determination of chemical safety in support advancing public health. Approaches including read-across, high-throughput screening, silico models, organ-on-a-chip technologies were addressed a 2017 workshop focusing on how scientists can move effectively toward vision 21st century food assessments. The application these alternative methods, need further...
High-throughput in vitro assays and exposure prediction efforts are paving the way for modeling chemical risk; however, utility of such extensive datasets can be limited or misleading when annotation fails to capture current usage. To address this data gap provide context food-use United States (US), manual curation food-relevant chemicals ToxCast was conducted. Chemicals were categorized into three categories: (1) direct food additives, (2) indirect (3) pesticide residues. Manual resulted...
In vitro methods offer opportunities to provide mechanistic insight into bioactivity as well human-relevant toxicological assessments compared animal testing. One of the challenges for this task is putting in data an vivo exposure context, which extrapolation (IVIVE) translates clinically relevant metrics using reverse dosimetry. This study applies IVIVE approach toxicity assessment ingredients and their mixtures e-cigarette (EC) aerosols a case study. Reported cytotoxicity EC aerosols,...