A5019518514- Contact Dermatitis and Allergies
- Animal testing and alternatives
- Computational Drug Discovery Methods
- Carcinogens and Genotoxicity Assessment
- melanin and skin pigmentation
- Skin Protection and Aging
- Effects and risks of endocrine disrupting chemicals
- Occupational exposure and asthma
- Indoor Air Quality and Microbial Exposure
- Antimicrobial agents and applications
- Essential Oils and Antimicrobial Activity
- Pesticide Exposure and Toxicity
- Lipid metabolism and biosynthesis
- ATP Synthase and ATPases Research
- Chemistry and Chemical Engineering
- Animal Virus Infections Studies
- Medical Device Sterilization and Disinfection
- Biochemical Analysis and Sensing Techniques
- Air Quality and Health Impacts
- Identification and Quantification in Food
- Mitochondrial Function and Pathology
- Allergic Rhinitis and Sensitization
- Fungal and yeast genetics research
- Digital Imaging for Blood Diseases
- Inhalation and Respiratory Drug Delivery
United States Food and Drug Administration
2013-2025
Center for Devices and Radiological Health
2019-2025
Office of Science
2021-2025
Universidad de Bogotá Jorge Tadeo Lozano
2022
Center for Drug Evaluation and Research
2016-2019
Center for Food Safety and Applied Nutrition
2013-2017
University of Chicago
2001-2004
Molecular Oncology (United States)
2001-2004
Wayne State University
1995-2000
ABSTRACT Formaldehyde (FA) is a highly reactive aldehyde that regarded as an inhalation hazard and human carcinogen. Herein, we report follow‐up study evaluating the effects of exposure duration on toxicity mutagenicity FA using in vitro air‐liquid‐interface (ALI) airway tissue model. Previously exposed ALI cultures to 7.5, 15 30‐ppm fumes 4 h/day for 5 days; currently, have increased 7.5 ppm days/week weeks, followed by 28‐day recovery. Due its toxicity, 30 were treated days, Tissue...
Formaldehyde (FA) is an irritating, highly reactive aldehyde that widely regarded as asthmagen. In addition to its use in industrial applications and being a product of combustion reaction endogenous metabolism, FDA-regulated products may contain FA or release fumes present toxicity risks for both patients healthcare workers. Exposure airborne associated with nasal neoplastic lesions animals humans. It classified Group 1 carcinogen by International Agency Research on Cancer (IARC) based the...
Isothiazolinone biocides are broad‐spectrum preservatives that widely used in cosmetics, household, and industrial products. An increase the number of cases allergic contact dermatitis to isothiazolinone preservatives, namely, methylisothiazolinone methylchloroisothiazolinone, have been recently noticed. The Food Drug Administration relies on analytical methods quantify levels use cosmetic ingredients support enforcement action against products not compliance with law. In this study, an...
Abstract An HPLC-UV method was developed for the quantitative analysis of nine skin whitening agents in a single injection. These compounds are α-arbutin, β-arbutin, kojic acid, nicotinamide, resorcinol, ascorbic hydroquinone, 4-methoxyphenol, and 4-ethoxyphenol. The separation achieved on reversed-phase C18 column within 30 min. mobile phase composed water methanol, both containing 0.1% acetic acid (v/v). stability analytes evaluated at different pH values between 2.3 7.6, extraction...
Evaluating consumer products for potentially harmful side effects of chemical ingredients is important the protection both and those involved in manufacturing process. In order to assess risk potential chemicals, regulatory agencies have encouraged development several silico, vitro, chemico methods as alternatives eliminate or minimize use animals. To add structural information existing methods, an NMR-based method proposed probing reactivity classification electrophiles (E) using a model...
Tea tree oil (TTO) is an essential obtained from the leaves of Melaleuca alternifolia, M. linariifolia, or dissitiflora. Because commercial importance TTO, substitution adulteration with other tea species (such as cajeput, niaouli, manuka, kanuka oils) common and may pose significant risks along perceived health benefits. The distinctive nature, qualitative quantitative compositional variation these oils, responsible for various pharmacological well adverse effects. Authentic TTOs...
Glutaraldehyde (GA) has been cleared by the Center for Devices and Radiological Health (CDRH) of Food Drug Administration (FDA) as a high-level disinfectant disinfecting heat-sensitive medical equipment in hospitals healthcare facilities. Inhalation exposure to GA is known cause respiratory irritation sensitization animals humans. To reproduce some vivo effects elicited GA, we used liquid aerosol system evaluated tissue responses human vitro airway epithelial model. The cultures were treated...
Sunscreen products are predominantly regulated as over-the-counter (OTC) drugs by the US FDA. The "active" ingredients function ultraviolet filters. Once a sunscreen product is generally recognized safe and effective (GRASE) via an OTC drug review process, new formulations using these do not require FDA approval, however, majority of have never been tested to uncover any potential endocrine activity their ability interact with estrogen receptor (ER) unknown, despite fact that this very...
The aim of this study was to establish a comparative analysis the chemical and enzymatic stability α- β-arbutins as potential sources substance concern hydroquinone (HQ). performed using an array techniques including HPLC-PDA, nuclear magnetic resonance (NMR) optical rotation (OR). Both arbutins are emerging popular effective skin whiteners, acting tyrosinase inhibitors in fashion similar whitening agent HQ. Due their structural similarity regulated HQ, both may be regarded active aglycone...
The phospholipid cardiolipin (CL) is ubiquitous in eucaryotes and unique structure, subcellular localization, potential function. Previous studies have shown that CL associated with major respiratory complexes the mitochondrial membrane. To determine whether biosynthesis requires presence of intact complexes, we measured activity synthase, which catalyzes synthesis from cytidine diphosphate diacylglycerol phosphatidylglycerol, <i>Saccharomyces cerevisiae</i>strains genetic defects oxidative...
Ortho-phthalaldehyde (OPA) is a chemical disinfectant used for the high-level sterilization of heat-sensitive medical instruments. Although OPA considered safer alternative to glutaraldehyde, no exposure limits have been established respiratory exposures ensure safety and safe use OPA-treated In order address data gaps in toxicological profile OPA, we treated human vitro air–liquid-interface (ALI) airway cultures at air interface with various concentrations aerosols 10 consecutive days....
A phenological study was conducted to determine the impact of harvest maturity on immune-modulating properties Echinacea purpurea. The aerial parts this plant were collected during seven stages development and assayed for a common botanical marker species, cichoric acid. Plants selected also total polysaccharides compared their effects THP-1 monocyte/macrophage cell line by means gene expression study. Although concentration acid did not change significantly course study, stage 1 (advanced...
Abstract In the United States, a pre-market regulatory submission for any medical device that comes into contact with either patient or clinical practitioner must include an adequate toxicity evaluation of chemical substances can be released from during its intended use. These substances, also referred to as extractables and leachables, evaluated their potential induce sensitization/allergenicity, which traditionally has been done in animal assays such guinea pig maximization test (GPMT)....
Broad spectrum sunscreens with a sun protection factor (SPF) of 15 or greater are indicated to decrease the risk skin cancer and early aging caused by if used as directed other measures. To determine whether sunscreen product performance is compromised under solar exposure test spectral uniformity across UVA spectrum, we tested broad variety active pharmaceutical ingredients (APIs) in dosage forms.A cross-sectional market survey 32 drug products containing either organic inorganic APIs SPFs...
Abstract Mitochondrial cardiolipin synthase catalyzes the transfer of a phosphatidyl moiety from phosphatidyl‐CMP PtdCMP) to phosphatidylglycerol (PtdGro) in presence specific divalent cations. The was solubilized Saccharomyces cerevisiae mitochondria and purified about 300‐fold. partially enzyme part medium‐size, mixed micelle which had bind foreign substrate/detergent before catalysis could occur. kinetics were studied by changing molar fraction substrate micelles. obeyed Michaelis‐Menten...
Arbutin is the D-glucose derivative of aromatic compound hydroquinone. β-Arbutin occurs naturally in many species Ericaceae and Caprifoliaceae families. α-Arbutin commonly produced by chemical or biotechnological routes. Both β- α-arbutin became popular whitening agents because their ability to interfere with melanin synthesis skin, but they may represent a source hazardous hydroquinone vivo due decomposition. Because this risk, both compounds have been investigated for potential hydrolysis...