A5076309236- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Amino Acid Enzymes and Metabolism
- Biochemical and Molecular Research
- Polyamine Metabolism and Applications
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Microbial Community Ecology and Physiology
- Bioeconomy and Sustainability Development
- RNA and protein synthesis mechanisms
- bioluminescence and chemiluminescence research
- CRISPR and Genetic Engineering
- Plant nutrient uptake and metabolism
- Biocrusts and Microbial Ecology
- Enzyme Production and Characterization
- Microbial Natural Products and Biosynthesis
- Cancer Research and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Algal biology and biofuel production
- Transgenic Plants and Applications
National University of Singapore
2018-2025
Jiangnan University
2013-2016
Wuxi Institute of Technology
2016
Meijo University
2004-2006
2,5-Furandicarboxylic acid (FDCA) is an important renewable biotechnological building block because it serves as environmentally friendly substitute for terephthalic in the production of polyesters. Currently, FDCA produced mainly via chemical oxidation, which can cause severe environmental pollution. In this study, we developed process from 5-hydroxymethyl furfural (5-HMF) using a newly isolated strain, Raoultella ornithinolytica BF60. First, R. BF60 was identified by screening and...
Detecting alterations in plasmid structures is often performed using conventional molecular biology. However, these methods are laborious and time-consuming for studying the conditions inducing mutations, which prevent real-time access to cell heterogeneity during bioproduction. In this work, we propose combining both flow cytometry fluorescence-activated sorting, integrated with mechanistic modelling study that lead recombination a limonene-producing microbial system as case study. A gene...
Genome sequences of cyanobacteria, Synechocystis sp. PCC 6803, Anabaena 7120, and Thermosynechococcus elongatus BP-1 revealed the presence a single Ca2+/H+ antiporter in these organisms. Here, we isolated putative gene from 6803 (synCAX) as well homologous halotolerant cyanobacterium Aphanothece halophytica (apCAX). In contrast to plant vacuolar CAXs, full-length apCAX synCAX genes complemented Ca2+-sensitive phenotype an Escherichia coli mutant. ApCAX SynCAX proteins catalyzed specifically...
α-Keto-γ-methylthiobutyric acid (KMTB), a keto derivative of l-methionine, has great potential for use as an alternative to l-methionine in the poultry industry and anti-cancer drug. This study developed environment friendly process KMTB production from by Escherichia coli whole-cell biocatalyst expressing engineered l-amino deaminase (l-AAD) Proteus vulgaris. We first overexpressed P. vulgaris l-AAD E. BL21 (DE3) further optimized transformation process. The maximal molar conversion ratio...
Pyruvate, which has been widely used in the food, pharmaceutical, and agrochemical industries, can be produced by “one-step pyruvate production” method from<sc>d</sc>/<sc>l</sc>-alanine with a whole-cell<italic>E. coli</italic>biocatalyst expressing<sc>l</sc>-amino acid deaminase (pm1) from<italic>Proteus mirabilis</italic>.
Tobacco plants were transformed with a Nhap type Na+/H+ antiporter gene, SynnhaP1 (slr1595), from cyanobacterium Synechocystis sp. PCC 6803. Two kinds of promoters, Arabidopsis alcohol dehydrogenase gene promoter (Adh promoter) and CaMV 35S (35S promoter), used. The transgenic driven by Adh accumulated SynNhaP1 proteins only in root whereas the all tissues. Confocal imaging SynNhaP1-GFP fusion protein suggests intracellular localization plasma membrane. Transgenic exhibited higher...
Phenylpyruvic acid (PPA) is widely used in the pharmaceutical, food, and chemical industries. Here, a two-step bioconversion process, involving growing resting cells, was established to produce PPA from l-phenylalanine using engineered Escherichia coli constructed previously. First, biotransformation conditions for cells were optimized (l-phenylalanine concentration 20.0 g·L−1, temperature 35°C) two-stage control strategy (keep 20°C 12 h increase 35°C until end of biotransformation)...
Addressing urgent environmental challenges, this commentary emphasizes the need for green, bio-based solutions in chemical production from renewable feedstocks. It highlights advanced metabolic engineering of microbial strains and use consortia as innovative approaches efficient resource recovery. These strategies aim to enhance conversion diverse feedstocks, including agricultural residues, industrial by-products, greenhouse gases, into value-added chemicals. This article discusses...
<sc>l</sc>-Amino acid deaminases (LAADs; EC 1.4.3.2) belong to a family of amino dehydrogenases that catalyze the formation α-keto acids from<sc>l</sc>-amino acids.