A5001692959- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Biofuel production and bioconversion
- Microbial Natural Products and Biosynthesis
- Bacteriophages and microbial interactions
- Fermentation and Sensory Analysis
- Bioinformatics and Genomic Networks
- RNA and protein synthesis mechanisms
- Machine Learning in Bioinformatics
- Plant biochemistry and biosynthesis
Boston University
2022-2024
Imperial College London
2023
Swedish University of Agricultural Sciences
2011
Engineered microbial consortia often have enhanced system performance and robustness compared with single-strain biomanufacturing production platforms. However, few tools are available for generating co-cultures of the model key industrial host Saccharomyces cerevisiae. Here we engineer auxotrophic overexpression yeast strains that can be used to create through exchange essential metabolites. Using these as modules, engineered two- three-member using different cross-feeding architectures....
Abstract Nutritional codependence (syntrophy) has underexplored potential to improve biotechnological processes by using cooperating cell types. So far, design of yeast syntrophic communities required extensive genetic manipulation, as the co-inoculation most eukaryotic microbial auxotrophs does not result in cooperative growth. Here we employ high-throughput phenotypic screening systematically test pairwise combinations auxotrophic Saccharomyces cerevisiae deletion mutants. Although...
Aim: Testing the ability of alternative ethanol production yeast Dekkera bruxellensis to produce from lignocellulose hydrolysate and comparing it Saccharomyces cerevisiae. Methods Results: Industrial isolates D. S. cerevisiae were cultivated in small‐scale batch fermentations enzymatically hydrolysed steam exploded aspen sawdust. Different dilutions tested. None yeasts grew undiluted or 1 : 2 diluted [final glucose concentration always adjusted 40 g l−1 (0·22 mol l−1)]. This was most likely...
Heterologous production of limonene in microorganisms through the mevalonate (MVA) pathway has traditionally imposed metabolic burden and reduced cell fitness, where imbalanced stoichiometries among sequential enzymes result accumulation toxic intermediates. Although prior studies have shown that changes to mRNA stability, RBS strength, protein homology can be effective strategies for balancing enzyme levels MVA pathway, testing different variations these parameters often requires distinct...
Dense arrangements of binding sites within nucleotide sequences can collectively influence downstream transcription rates or initiate biomolecular interactions. For example, natural promoter regions harbor many overlapping factor that the rate initiation. Despite prevalence in nature, rapid design with remains a challenge. Here, we show this is an NP-hard problem, coined here as String Packing Problem (SPP). We then introduce computational technique efficiently assembles sets DNA-protein...