A5068916001- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- High voltage insulation and dielectric phenomena
- Power Transformer Diagnostics and Insulation
- Catalysis for Biomass Conversion
- Lipid metabolism and biosynthesis
- Lightning and Electromagnetic Phenomena
- Viral Infectious Diseases and Gene Expression in Insects
- Fungal and yeast genetics research
- Power Systems Fault Detection
- Aerosol Filtration and Electrostatic Precipitation
- 3D Printing in Biomedical Research
- Biochemical and biochemical processes
- Plant Gene Expression Analysis
- Advanced Cellulose Research Studies
- Electrohydrodynamics and Fluid Dynamics
- Proteins in Food Systems
- Innovative Microfluidic and Catalytic Techniques Innovation
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Microencapsulation and Drying Processes
- CRISPR and Genetic Engineering
- Gene Regulatory Network Analysis
- Fluid Dynamics and Mixing
Imperial College London
2019-2025
The University of Queensland
2024-2025
Foundation Center
2024
Novo Nordisk Foundation
2022-2024
Technical University of Denmark
2022-2024
State Grid Corporation of China (China)
2024
Shanghai Electric (China)
2017-2024
Nanjing Tech University
2024
Monash University
2017-2022
Fuzhou University
2022
Engineered microbial consortia often have enhanced system performance and robustness compared with single-strain biomanufacturing production platforms. However, few tools are available for generating co-cultures of the model key industrial host Saccharomyces cerevisiae. Here we engineer auxotrophic overexpression yeast strains that can be used to create through exchange essential metabolites. Using these as modules, engineered two- three-member using different cross-feeding architectures....
Abstract Nutritional codependence (syntrophy) has underexplored potential to improve biotechnological processes by using cooperating cell types. So far, design of yeast syntrophic communities required extensive genetic manipulation, as the co-inoculation most eukaryotic microbial auxotrophs does not result in cooperative growth. Here we employ high-throughput phenotypic screening systematically test pairwise combinations auxotrophic Saccharomyces cerevisiae deletion mutants. Although...
DELLA proteins are negative regulators of the gibberellin response pathway in angiosperms, acting as central hubs that interact with hundreds transcription factors (TFs) and to modulate their activities. While mechanism TF sequestration by DELLAs prevent DNA binding downstream targets has been extensively documented, allows them act coactivators remains be understood. Here, we demonstrate directly recruit Mediator complex specific loci Arabidopsis, facilitating transcription. This...
Solid-state fermentation (SSF) has regained interest owing to its advantages in solid waste treatment and industries. However, heterogeneous heat mass transfer are often caused by the absence of free water noticeable loss from microbial utilization moisture evaporation SSF. It is necessary explore more effective ways solve issues supplement SSF based on online capillary monitoring, because dominant form that present lost substrate. Two novel capillary-water supply strategies were proposed,...
Microbial-derived aromatics provide a sustainable and renewable alternative to petroleum-derived chemicals. In this study, we used the model yeast Saccharomyces cerevisiae produce aromatic molecules by exploiting concept of modularity in synthetic biology. Three different modular approaches were investigated for production valuable fragrance raspberry ketone (RK), found fruits mostly produced from petrochemicals. The first strategy was cloning, which enabled generation combinatorial...
Cycloalkanes have broad applications as specialty fuels, lubricants, and pharmaceuticals but are not currently available from renewable sources, whereas, production of microbial cycloalkanes such cyclopropane fatty acids (CFA) has bottlenecks. Here, a systematic investigation was undertaken into the biosynthesis CFA in Saccharomyces cerevisiae heterologously expressing bacterial synthase. The enzyme catalyzes formation 3-membered ring unsaturated acids. Monounsaturated phospholipids (PL)...
Biodiesel is a valuable renewable fuel made from derivatized fatty acids produced in plants, animals, and oleaginous microbes. Of the latter, yeasts are of special interest due to their wide use biotechnology, ability synthesize store large amounts triacylglycerols while utilizing non-food carbon sources. While yeast efficiently produce lipids, genetic modification indeed, lipid pathway metabolic engineering, usually required for cost-effective production. Traditionally, gas chromatography...
Corynebacterium glutamicum has industrial track records for producing a variety of valuable products such as amino acids. Although CRISPR-based genome editing technologies have undergone immense developments in recent years, the suicide-plasmid-based approaches are still predominant C. manipulation. It is crucial to develop simple and efficient CRISPR method glutamicum.In this study, we developed RecombinAtion Prior Induced Double-strand-break (RAPID) technology glutamicum, Cpf1 cleavage was...
Microorganisms can be engineered to sustainably produce a variety of products including fuels, pharmaceuticals, materials, and food. However, highly strains often result in low production yield, due undesired effects such as metabolic burden the toxicity intermediates. Drawing inspiration from natural ecosystems, construction synthetic community with division labor offer advantages for bioproduction. This approach involves dividing specific tasks among members, thereby enhancing...
For achieving the aging characteristic parameters of transformer oil-paper insulation, oil impregnated pressboard samples are acceleratedly thermally aged at high temperature (~150°C) in laboratory, and on same sample before after being aged, polarization depolarization current (PDC) tests performed different temperatures (30, 40, 50 60°C), respectively. Besides analyzing PDC results, differential time-domain spectroscopy (DTDS) technique is introduced to investigate status insulation...
Monoterpene indole alkaloids (MIAs) make up a highly bioactive class of metabolites produced by range tropical and subtropical plants. The corynanthe-type MIAs are stereochemically complex subclass with therapeutic potential against large number indications including cancer, psychotic disorders, erectile dysfunction. Here, we report yeast-based cell factories capable de novo production rauwolscine, yohimbine, tetrahydroalstonine, corynanthine. From this, demonstrate regioselective...
l-Valine, an essential amino acid, serves as a valuable compound in various industries. However, engineering strains with both high yield and purity are yet to be delivered for microbial l-valine production. We engineered
This paper mainly discussed the utilization of transient Earth voltage measurement (TEVM) in partial discharge (PD) detection gas insulation switchgear. Four typical artificial defect models SF <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">6</sub> such as spikes fixed on high (HV) conductor, suspended metals, voids dielectric, and metal particles surface dielectric had been used test objects ac PD experiments with aid synchronized signals...
As promising alternatives to fossil-derived oils, microbial lipids are important as industrial feedstocks for biofuels and oleochemicals. Our broad aim is increase lipid content in oleaginous yeast through expression of accumulation genes use Saccharomyces cerevisiae functionally assess obtained from oil-producing plants microalgae. Lipid DGAT (diacylglycerol acyltransferase), PDAT (phospholipid: diacylglycerol ROD1 (phosphatidylcholine: choline-phosphotransferase) were separately expressed...